The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris
This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two ways. The protoplasts of A. cinnamomea were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm, and the protoplasts of C. militaris were inactivated by UV r...
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Tsinghua University Press
2022-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000702 |
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| author | Wei-Kuang Lai Yang-Chang Wu Tai-Sheng Yeh Chun-Ren Hsieh Yi-Hong Tsai Chien-Kei Wei Chi-Ying Li Ying-Chen Lu Fang-Rong Chang |
| author_facet | Wei-Kuang Lai Yang-Chang Wu Tai-Sheng Yeh Chun-Ren Hsieh Yi-Hong Tsai Chien-Kei Wei Chi-Ying Li Ying-Chen Lu Fang-Rong Chang |
| author_sort | Wei-Kuang Lai |
| collection | DOAJ |
| description | This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two ways. The protoplasts of A. cinnamomea were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm, and the protoplasts of C. militaris were inactivated by UV radiation to invalidate their genome function, then they were fused under optimal conditions to get a fusion rate as (7.42 ± 0.8) × 10-6 fusants/mL; the new fusants were abbreviated as Ac-Cm. On the other hand, when A. cinnamomea and C. militaris were treated with heat and UV oppositely using similar experiments, the fusion rate was (9.70 ± 0.68) × 10-5 fusants/mL, and the new fusants were abbreviated as Cm-Ac. We selected each of two best-growing fused colonies Ac-Cm-1, Ac-Cm-2, Cm-Ac-1, and Cm-Ac-2, together with parental A. cinnamomea and C. militaris, and studied their morphology, growth antagonism tests, and genetic relationships by 18S rRNA sequencing. In comparison with the initial cultures of 4 fusants, the yields of adenosine, biomass, cordycepic acid, cordycepin, total polysaccharide, and total triterpenoids were increased up 1.305−50.1563 times in the optimal medium conditions. For gene stability tests, those of the four fusants and their outputs were stabilized within 10 generations. |
| format | Article |
| id | doaj-art-cff726b263ef4cc68400314d3e0ea2d7 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-cff726b263ef4cc68400314d3e0ea2d72025-02-03T06:15:29ZengTsinghua University PressFood Science and Human Wellness2213-45302022-09-0111512401251The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militarisWei-Kuang Lai0Yang-Chang Wu1Tai-Sheng Yeh2Chun-Ren Hsieh3Yi-Hong Tsai4Chien-Kei Wei5Chi-Ying Li6Ying-Chen Lu7Fang-Rong Chang8Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ChinaGraduate Institute of Integrated Medicine, China Medical University, Taichung 404, Taiwan, China; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan, ChinaDepartment of Food Science and Nutrition, Meiho University, Pingtung 912, Taiwan, ChinaDepartment of Food Science, National Chiayi University, Chiayi City 600, Taiwan, ChinaGraduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ChinaGraduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ChinaGraduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ChinaDepartment of Food Science, National Chiayi University, Chiayi City 600, Taiwan, China; Corresponding author. Fax: +886-5-2717596.Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Departmenth Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan, China; Corresponding author at: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China, Fax: +886-7-3114773.This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two ways. The protoplasts of A. cinnamomea were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm, and the protoplasts of C. militaris were inactivated by UV radiation to invalidate their genome function, then they were fused under optimal conditions to get a fusion rate as (7.42 ± 0.8) × 10-6 fusants/mL; the new fusants were abbreviated as Ac-Cm. On the other hand, when A. cinnamomea and C. militaris were treated with heat and UV oppositely using similar experiments, the fusion rate was (9.70 ± 0.68) × 10-5 fusants/mL, and the new fusants were abbreviated as Cm-Ac. We selected each of two best-growing fused colonies Ac-Cm-1, Ac-Cm-2, Cm-Ac-1, and Cm-Ac-2, together with parental A. cinnamomea and C. militaris, and studied their morphology, growth antagonism tests, and genetic relationships by 18S rRNA sequencing. In comparison with the initial cultures of 4 fusants, the yields of adenosine, biomass, cordycepic acid, cordycepin, total polysaccharide, and total triterpenoids were increased up 1.305−50.1563 times in the optimal medium conditions. For gene stability tests, those of the four fusants and their outputs were stabilized within 10 generations.http://www.sciencedirect.com/science/article/pii/S2213453022000702Medicinal fungiProtoplast fusionAntrodia cinnamomeaCordyceps militarisSecondary metabolite |
| spellingShingle | Wei-Kuang Lai Yang-Chang Wu Tai-Sheng Yeh Chun-Ren Hsieh Yi-Hong Tsai Chien-Kei Wei Chi-Ying Li Ying-Chen Lu Fang-Rong Chang The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris Food Science and Human Wellness Medicinal fungi Protoplast fusion Antrodia cinnamomea Cordyceps militaris Secondary metabolite |
| title | The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris |
| title_full | The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris |
| title_fullStr | The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris |
| title_full_unstemmed | The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris |
| title_short | The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris |
| title_sort | protoplast two way fusions and fusant characteristics of antrodia cinnamomea and cordyceps militaris |
| topic | Medicinal fungi Protoplast fusion Antrodia cinnamomea Cordyceps militaris Secondary metabolite |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000702 |
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