Oxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart disease
BackgroundEarly life gut microbiota is known to shape the immune system and has a crucial role in immune homeostasis. Only little is known about composition and dynamics of the intestinal microbiota in infants with congenital heart disease (CHD) and potential influencing factors.MethodsWe evaluated...
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Frontiers Media S.A.
2025-01-01
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| author | Hanna Renk Hanna Renk Hanna Renk Ulrich Schoppmeier Jennifer Müller Vanessa Kuger Felix Neunhoeffer Christian Gille Silke Peter Silke Peter |
| author_facet | Hanna Renk Hanna Renk Hanna Renk Ulrich Schoppmeier Jennifer Müller Vanessa Kuger Felix Neunhoeffer Christian Gille Silke Peter Silke Peter |
| author_sort | Hanna Renk |
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| description | BackgroundEarly life gut microbiota is known to shape the immune system and has a crucial role in immune homeostasis. Only little is known about composition and dynamics of the intestinal microbiota in infants with congenital heart disease (CHD) and potential influencing factors.MethodsWe evaluated the intestinal microbial composition of neonates with CHD (n = 13) compared to healthy controls (HC, n = 30). Fecal samples were analyzed by shotgun metagenomics. Different approaches of statistical modeling were applied to assess the impact of influencing factors on variation in species composition. Unsupervised hierarchical clustering of the microbial composition of neonates with CHD was used to detect associations of distinct clusters with intestinal tissue oxygenation and perfusion parameters, obtained by the “oxygen to see” (O2C) method.ResultsOverall, neonates with CHD showed an intestinal core microbiota dominated by the genera Enterococcus (27%) and Staphylococcus (20%). Furthermore, a lower abundance of the genera Bacteroides (8% vs. 14%), Parabacteroides (1% vs. 3%), Bifidobacterium (4% vs. 12%), and Escherichia (8% vs. 23%) was observed in CHD compared to HCs. CHD patients that were born by vaginal delivery showed a lower fraction of the genera Bacteroides (15% vs. 21%) and Bifidobacterium (7% vs. 22%) compared to HCs and in those born by cesarean section, these genera were not found at all. In infants with CHD, we found a significant impact of oxygen saturation (SpO2) on relative abundances of the intestinal core microbiota by multivariate analysis of variance (F[8,2] = 24.9, p = 0.04). Statistical modeling suggested a large proportional shift from a microbiota dominated by the genus Streptococcus (50%) in conditions with low SpO2 towards the genus Enterococcus (61%) in conditions with high SpO2. We identified three distinct compositional microbial clusters, corresponding neonates differed significantly in intestinal blood flow and global gut perfusion.ConclusionEarly life differences in gut microbiota of CHD neonates versus HCs are possibly linked to oxygen levels. Delivery method may affect microbiota stability. However, further studies are needed to assess the effect of potential interventions including probiotics or fecal transplants on early life microbiota perturbations in neonates with CHD. |
| format | Article |
| id | doaj-art-cff07506195d4ba6b8ddaa1f7ef8f9f4 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-cff07506195d4ba6b8ddaa1f7ef8f9f42025-08-20T02:59:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14688421468842Oxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart diseaseHanna Renk0Hanna Renk1Hanna Renk2Ulrich Schoppmeier3Jennifer Müller4Vanessa Kuger5Felix Neunhoeffer6Christian Gille7Silke Peter8Silke Peter9Department of Neuropediatrics, Developmental Neurology and Social Pediatrics, University Children’s Hospital Tübingen, Tübingen, GermanyGerman Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, GermanyDepartment of Pediatric Cardiology, Pulmonology and Pediatric Intensive Care Medicine, University Children’s Hospital Tübingen, Tübingen, GermanyInstitute of Medical Microbiology and Hygiene, University of Tübingen, Tübingen, GermanyNGS Competence Center Tübingen (NCCT), University of Tübingen, Tübingen, GermanyDepartment of Pediatric Cardiology, Pulmonology and Pediatric Intensive Care Medicine, University Children’s Hospital Tübingen, Tübingen, GermanyDepartment of Pediatric Cardiology, Pulmonology and Pediatric Intensive Care Medicine, University Children’s Hospital Tübingen, Tübingen, GermanyDepartment of Neonatology, Heidelberg University Children’s Hospital, Heidelberg, GermanyGerman Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, GermanyInstitute of Medical Microbiology and Hygiene, University of Tübingen, Tübingen, GermanyBackgroundEarly life gut microbiota is known to shape the immune system and has a crucial role in immune homeostasis. Only little is known about composition and dynamics of the intestinal microbiota in infants with congenital heart disease (CHD) and potential influencing factors.MethodsWe evaluated the intestinal microbial composition of neonates with CHD (n = 13) compared to healthy controls (HC, n = 30). Fecal samples were analyzed by shotgun metagenomics. Different approaches of statistical modeling were applied to assess the impact of influencing factors on variation in species composition. Unsupervised hierarchical clustering of the microbial composition of neonates with CHD was used to detect associations of distinct clusters with intestinal tissue oxygenation and perfusion parameters, obtained by the “oxygen to see” (O2C) method.ResultsOverall, neonates with CHD showed an intestinal core microbiota dominated by the genera Enterococcus (27%) and Staphylococcus (20%). Furthermore, a lower abundance of the genera Bacteroides (8% vs. 14%), Parabacteroides (1% vs. 3%), Bifidobacterium (4% vs. 12%), and Escherichia (8% vs. 23%) was observed in CHD compared to HCs. CHD patients that were born by vaginal delivery showed a lower fraction of the genera Bacteroides (15% vs. 21%) and Bifidobacterium (7% vs. 22%) compared to HCs and in those born by cesarean section, these genera were not found at all. In infants with CHD, we found a significant impact of oxygen saturation (SpO2) on relative abundances of the intestinal core microbiota by multivariate analysis of variance (F[8,2] = 24.9, p = 0.04). Statistical modeling suggested a large proportional shift from a microbiota dominated by the genus Streptococcus (50%) in conditions with low SpO2 towards the genus Enterococcus (61%) in conditions with high SpO2. We identified three distinct compositional microbial clusters, corresponding neonates differed significantly in intestinal blood flow and global gut perfusion.ConclusionEarly life differences in gut microbiota of CHD neonates versus HCs are possibly linked to oxygen levels. Delivery method may affect microbiota stability. However, further studies are needed to assess the effect of potential interventions including probiotics or fecal transplants on early life microbiota perturbations in neonates with CHD.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1468842/fullcongenital heart diseaseintestinal microbiotanext-generation sequencingoxygen to seeO2Coxygenation |
| spellingShingle | Hanna Renk Hanna Renk Hanna Renk Ulrich Schoppmeier Jennifer Müller Vanessa Kuger Felix Neunhoeffer Christian Gille Silke Peter Silke Peter Oxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart disease Frontiers in Microbiology congenital heart disease intestinal microbiota next-generation sequencing oxygen to see O2C oxygenation |
| title | Oxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart disease |
| title_full | Oxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart disease |
| title_fullStr | Oxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart disease |
| title_full_unstemmed | Oxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart disease |
| title_short | Oxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart disease |
| title_sort | oxygenation and intestinal perfusion and its association with perturbations of the early life gut microbiota composition of children with congenital heart disease |
| topic | congenital heart disease intestinal microbiota next-generation sequencing oxygen to see O2C oxygenation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1468842/full |
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