Hepatoprotective effects of propolis ethanol extract on D-galactose-induced aging mice: A metabolomics and transcriptomics analysis focusing on insulin resistance pathways
This study aimed to evaluate the hepatoprotective effects of Propolis ethanol extract (PEE) in D-galactose (D-gal)-induced senescence mice and explore its underlying mechanisms. Mice were administered PEE by gavage, and body weight, organ index, and serum biomarkers (SOD, MDA, AST, ALT) were measure...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000106 |
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| author | Jinjia Liu Songhao Tian Jixiang Wang Qing Sang Wenting Su Jinqiang Wu Fenglei Zai |
| author_facet | Jinjia Liu Songhao Tian Jixiang Wang Qing Sang Wenting Su Jinqiang Wu Fenglei Zai |
| author_sort | Jinjia Liu |
| collection | DOAJ |
| description | This study aimed to evaluate the hepatoprotective effects of Propolis ethanol extract (PEE) in D-galactose (D-gal)-induced senescence mice and explore its underlying mechanisms. Mice were administered PEE by gavage, and body weight, organ index, and serum biomarkers (SOD, MDA, AST, ALT) were measured. Liver histopathological changes were examined using HE staining. Additionally, transcriptomics and metabolomics analyses were employed to elucidate the molecular mechanisms. The results demonstrated that PEE ameliorated the reductions in body weight and liver index in aged mice. PEE significantly increased serum SOD levels and reduced MDA, AST, and ALT levels. Histological analysis revealed that PEE mitigated liver damage. Mechanistically, PEE appeared to exert hepatoprotective effects by modulating the insulin resistance pathway, particularly through the PI3K-AKT/PP1 signaling axis, thereby enhancing glycogen synthesis in the damaged liver. These findings provide a scientific foundation for the therapeutic potential of PEE in anti-aging and liver disease. |
| format | Article |
| id | doaj-art-cfecd10481494ad1a44a239f7d4829f7 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-cfecd10481494ad1a44a239f7d4829f72025-02-09T04:59:54ZengElsevierJournal of Functional Foods1756-46462025-02-01125106668Hepatoprotective effects of propolis ethanol extract on D-galactose-induced aging mice: A metabolomics and transcriptomics analysis focusing on insulin resistance pathwaysJinjia Liu0Songhao Tian1Jixiang Wang2Qing Sang3Wenting Su4Jinqiang Wu5Fenglei Zai6Department of Biochemistry, Basic Medical College, Changzhi Medical College, Changzhi, Shanxi 046000, ChinaDepartment of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang, Shanxi 032200, ChinaDepartment of Epidemiology and Health Statistics of Public Health and Preventive Medicine, Changzhi Medical College, Changzhi 046000, China; Shanxi Higher Education Institutions of Science and Technology Innovation Plan Platform, Laboratory of Environmental Factors and Population Health, Changzhi 046000, China; Key Laboratory of Environmental Pathogenic Mechanisms and Prevention of Chronic Diseases, Changzhi Medical College, Changzhi 046000, ChinaDepartment of Rehabilitation Medicine, Changzhi Medical College, Changzhi, Shanxi 046000, ChinaDepartment of Physiology, Basic Medical College, Changzhi Medical College, Changzhi, Shanxi 046000, ChinaDepartment of Histology and Embryology, Basic Medical College, Changzhi Medical College, Changzhi, Shanxi 046000, ChinaDepartment of Rehabilitation Medicine, Changzhi Medical College, Changzhi, Shanxi 046000, China; Corresponding author.This study aimed to evaluate the hepatoprotective effects of Propolis ethanol extract (PEE) in D-galactose (D-gal)-induced senescence mice and explore its underlying mechanisms. Mice were administered PEE by gavage, and body weight, organ index, and serum biomarkers (SOD, MDA, AST, ALT) were measured. Liver histopathological changes were examined using HE staining. Additionally, transcriptomics and metabolomics analyses were employed to elucidate the molecular mechanisms. The results demonstrated that PEE ameliorated the reductions in body weight and liver index in aged mice. PEE significantly increased serum SOD levels and reduced MDA, AST, and ALT levels. Histological analysis revealed that PEE mitigated liver damage. Mechanistically, PEE appeared to exert hepatoprotective effects by modulating the insulin resistance pathway, particularly through the PI3K-AKT/PP1 signaling axis, thereby enhancing glycogen synthesis in the damaged liver. These findings provide a scientific foundation for the therapeutic potential of PEE in anti-aging and liver disease.http://www.sciencedirect.com/science/article/pii/S1756464625000106Propolis ethanol extractsAgingHepatoprotectionInsulin resistanceTranscriptomicsMetabolomics |
| spellingShingle | Jinjia Liu Songhao Tian Jixiang Wang Qing Sang Wenting Su Jinqiang Wu Fenglei Zai Hepatoprotective effects of propolis ethanol extract on D-galactose-induced aging mice: A metabolomics and transcriptomics analysis focusing on insulin resistance pathways Journal of Functional Foods Propolis ethanol extracts Aging Hepatoprotection Insulin resistance Transcriptomics Metabolomics |
| title | Hepatoprotective effects of propolis ethanol extract on D-galactose-induced aging mice: A metabolomics and transcriptomics analysis focusing on insulin resistance pathways |
| title_full | Hepatoprotective effects of propolis ethanol extract on D-galactose-induced aging mice: A metabolomics and transcriptomics analysis focusing on insulin resistance pathways |
| title_fullStr | Hepatoprotective effects of propolis ethanol extract on D-galactose-induced aging mice: A metabolomics and transcriptomics analysis focusing on insulin resistance pathways |
| title_full_unstemmed | Hepatoprotective effects of propolis ethanol extract on D-galactose-induced aging mice: A metabolomics and transcriptomics analysis focusing on insulin resistance pathways |
| title_short | Hepatoprotective effects of propolis ethanol extract on D-galactose-induced aging mice: A metabolomics and transcriptomics analysis focusing on insulin resistance pathways |
| title_sort | hepatoprotective effects of propolis ethanol extract on d galactose induced aging mice a metabolomics and transcriptomics analysis focusing on insulin resistance pathways |
| topic | Propolis ethanol extracts Aging Hepatoprotection Insulin resistance Transcriptomics Metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625000106 |
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