Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization
Holotomograhic microscopy (HTM) has emerged as a non-invasive imaging technique that offers high-resolution, quantitative 3D imaging of biological samples. This study explores the application of HTM in examining endothelial cells (ECs). HTM overcomes the limitations of traditional microscopy methods...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933525000172 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850103202584723456 |
|---|---|
| author | William D. Leineweber Gabriela Acevedo Munares Christian Leycam Raul Michael Juliette Noyer Patrick Jurney |
| author_facet | William D. Leineweber Gabriela Acevedo Munares Christian Leycam Raul Michael Juliette Noyer Patrick Jurney |
| author_sort | William D. Leineweber |
| collection | DOAJ |
| description | Holotomograhic microscopy (HTM) has emerged as a non-invasive imaging technique that offers high-resolution, quantitative 3D imaging of biological samples. This study explores the application of HTM in examining endothelial cells (ECs). HTM overcomes the limitations of traditional microscopy methods in capturing the real-time dynamics of ECs by leveraging the refractive index (RI) to map 3D distributions label-free. This work demonstrates the utility of HTM in visualizing key cellular processes during endothelialization, wherein ECs anchor, adhere, migrate, and proliferate. Leveraging the high resolution and quantitative power of HTM, we show that lipid droplets and mitochondria are readily visualized, enabling more comprehensive studies on their respective roles during endothelialization. The study highlights how HTM on a commercial instrument can uncover novel insights into HUVEC cell behavior, offering potential applications in medical diagnostics and research, particularly in developing treatments for cardiovascular diseases. This advanced imaging technique not only enhances our understanding of EC biology but also presents a significant step forward in the study of cardiovascular diseases, providing a robust platform for future research and therapeutic development. |
| format | Article |
| id | doaj-art-cfde0b72d907457b8864c1dbaffdbe98 |
| institution | DOAJ |
| issn | 0171-9335 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj-art-cfde0b72d907457b8864c1dbaffdbe982025-08-20T02:39:35ZengElsevierEuropean Journal of Cell Biology0171-93352025-06-01104215149210.1016/j.ejcb.2025.151492Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelializationWilliam D. Leineweber0Gabriela Acevedo Munares1Christian Leycam2Raul Michael3Juliette Noyer4Patrick Jurney5Stanford University, Department of Bioengineering, USASan Jose State University, Biomedical Engineering Department, USASan Jose State University, Biomedical Engineering Department, USASan Jose State University, Biomedical Engineering Department, USASan Jose State University, Biomedical Engineering Department, USASan Jose State University, Biomedical Engineering Department, USA; Corresponding author.Holotomograhic microscopy (HTM) has emerged as a non-invasive imaging technique that offers high-resolution, quantitative 3D imaging of biological samples. This study explores the application of HTM in examining endothelial cells (ECs). HTM overcomes the limitations of traditional microscopy methods in capturing the real-time dynamics of ECs by leveraging the refractive index (RI) to map 3D distributions label-free. This work demonstrates the utility of HTM in visualizing key cellular processes during endothelialization, wherein ECs anchor, adhere, migrate, and proliferate. Leveraging the high resolution and quantitative power of HTM, we show that lipid droplets and mitochondria are readily visualized, enabling more comprehensive studies on their respective roles during endothelialization. The study highlights how HTM on a commercial instrument can uncover novel insights into HUVEC cell behavior, offering potential applications in medical diagnostics and research, particularly in developing treatments for cardiovascular diseases. This advanced imaging technique not only enhances our understanding of EC biology but also presents a significant step forward in the study of cardiovascular diseases, providing a robust platform for future research and therapeutic development.http://www.sciencedirect.com/science/article/pii/S0171933525000172Holotomographic microscopyLabel-free imagingEndothelializationEndothelial cellsRefractive indexCell motility |
| spellingShingle | William D. Leineweber Gabriela Acevedo Munares Christian Leycam Raul Michael Juliette Noyer Patrick Jurney Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization European Journal of Cell Biology Holotomographic microscopy Label-free imaging Endothelialization Endothelial cells Refractive index Cell motility |
| title | Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization |
| title_full | Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization |
| title_fullStr | Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization |
| title_full_unstemmed | Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization |
| title_short | Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization |
| title_sort | holotomographic microscopy reveals label free quantitative dynamics of endothelial cells during endothelialization |
| topic | Holotomographic microscopy Label-free imaging Endothelialization Endothelial cells Refractive index Cell motility |
| url | http://www.sciencedirect.com/science/article/pii/S0171933525000172 |
| work_keys_str_mv | AT williamdleineweber holotomographicmicroscopyrevealslabelfreequantitativedynamicsofendothelialcellsduringendothelialization AT gabrielaacevedomunares holotomographicmicroscopyrevealslabelfreequantitativedynamicsofendothelialcellsduringendothelialization AT christianleycam holotomographicmicroscopyrevealslabelfreequantitativedynamicsofendothelialcellsduringendothelialization AT raulmichael holotomographicmicroscopyrevealslabelfreequantitativedynamicsofendothelialcellsduringendothelialization AT juliettenoyer holotomographicmicroscopyrevealslabelfreequantitativedynamicsofendothelialcellsduringendothelialization AT patrickjurney holotomographicmicroscopyrevealslabelfreequantitativedynamicsofendothelialcellsduringendothelialization |