Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk
The aim of this study was the isolation, identification, phylogenetic analysis and antifungal susceptibility of Candida spp. from milk samples of healthy and mastitic cows in Kayseri/Turkey. Milk samples from 300 cows were found to be negative/positive for mastitis with the California Mastitis Test....
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Croatian Dairy Union
2020-01-01
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| Series: | Mljekarstvo |
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| Online Access: | https://hrcak.srce.hr/file/337367 |
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| author | Harun Hizlisoy Nurhan Ertas Onmaz Serhat Al Fulden Karadal Yeliz Yildirim Zafer Gonulalan K. Semih Gumussoy Gonca Demir Aydemir Filiz Kasap Tekinsen Gokcen Dinc |
| author_facet | Harun Hizlisoy Nurhan Ertas Onmaz Serhat Al Fulden Karadal Yeliz Yildirim Zafer Gonulalan K. Semih Gumussoy Gonca Demir Aydemir Filiz Kasap Tekinsen Gokcen Dinc |
| author_sort | Harun Hizlisoy |
| collection | DOAJ |
| description | The aim of this study was the isolation, identification, phylogenetic analysis and antifungal susceptibility of Candida spp. from milk samples of healthy and mastitic cows in Kayseri/Turkey. Milk samples from 300 cows were found to be negative/positive for mastitis with the California Mastitis Test. Candida spp. was isolated by using the Brillance Candida Agar Base. Phenotypic tests, Matrix Assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF), and VITEK-2 analyses were applied to confirm the obtained isolates. Resistance to flucytosine, fluconazole and caspofungin antifungals of the isolates were determined by Etest and VITEK-2. The genetic homologies of Candida spp. isolates were determined by Repetitive Extragenic Palindromic-PCR (RepPCR). In this study 62 (from 53 healthy, 9 mastitic) yeast isolates were obtained and 37 (59.6 %) were identified as non-albicans Candida (NAC) species. Phenotypic tests revealed that out of 62 isolates, 29 (46.7 %), 4 (6.5 %), 3 (4.8 %), 1 (1.6 %) and, 24 (38.7 %) were identified as Candida lusitaniae, Candida catenulate, Candida tropicalis, Candida silvicola and other yeast species, respectively. Only one sample (1.6 %) was identified as Candida albicans by MALDI-TOF however, according to VITEK-2, the agent was not confirmed as C. albicans. According to antifungal susceptibility testing by VITEK-2, one (2.7 %) of the isolates was resistant to fluconazole, one (2.7 %) was resistant to caspofungin, and 4 (10.8 %) were resistant to flucytosine. However, using E test, 10 isolates (27 %) were resistant to flucytosine. Using Rep-PCR, eight genotypic clones were observed. Genotype F (13.8 %) and G (13.8 %; 2 subtypes) were common clones in this study. In conclusion, NAC species were detected in healthy and mastitic cow milk samples. Epidemiological studies need to be conducted to track effectively the main source and to understand the diversity and distribution of the agent. It is necessary to consider the potential risks of yeast contamination in milk for public health. It is essential to focus on adequate sanitation procedures and storage conditions of milk. |
| format | Article |
| id | doaj-art-cfdb112395d74f7885995ba79140ff2e |
| institution | DOAJ |
| issn | 0026-704X 1846-4025 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Croatian Dairy Union |
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| series | Mljekarstvo |
| spelling | doaj-art-cfdb112395d74f7885995ba79140ff2e2025-08-20T03:19:46ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252020-01-01701404910.15567/mljekarstvo.2020.0104Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milkHarun Hizlisoy0Nurhan Ertas Onmaz1Serhat Al2Fulden Karadal3Yeliz Yildirim4Zafer Gonulalan5K. Semih Gumussoy6Gonca Demir Aydemir7Filiz Kasap Tekinsen8Gokcen Dinc9Erciyes University, Faculty of Veterinary Medicine, Department of Veterinary Public Health, 38039-Kayseri, TurkeyErciyes University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, 38039-Kayseri, TurkeyErciyes University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, 38039-Kayseri, TurkeyOmer Halisdemir University, Bor Vocational School, Department of Food Processing, Dairy Products and Technology, 51700 Nigde, TurkeyErciyes University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, 38039-Kayseri, TurkeyErciyes University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, 38039-Kayseri, TurkeyErciyes University, Faculty of Veterinary Medicine, Department of Microbiology, 38039-Kayseri, TurkeyErciyes University, Faculty of Medicine, Department of Medical Microbiology, 38039-Kayseri, TurkeyErciyes University, Faculty of Medicine, Department of Medical Microbiology, 38039-Kayseri, TurkeyErciyes University, Faculty of Medicine, Department of Medical Microbiology, 38039-Kayseri, TurkeyThe aim of this study was the isolation, identification, phylogenetic analysis and antifungal susceptibility of Candida spp. from milk samples of healthy and mastitic cows in Kayseri/Turkey. Milk samples from 300 cows were found to be negative/positive for mastitis with the California Mastitis Test. Candida spp. was isolated by using the Brillance Candida Agar Base. Phenotypic tests, Matrix Assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF), and VITEK-2 analyses were applied to confirm the obtained isolates. Resistance to flucytosine, fluconazole and caspofungin antifungals of the isolates were determined by Etest and VITEK-2. The genetic homologies of Candida spp. isolates were determined by Repetitive Extragenic Palindromic-PCR (RepPCR). In this study 62 (from 53 healthy, 9 mastitic) yeast isolates were obtained and 37 (59.6 %) were identified as non-albicans Candida (NAC) species. Phenotypic tests revealed that out of 62 isolates, 29 (46.7 %), 4 (6.5 %), 3 (4.8 %), 1 (1.6 %) and, 24 (38.7 %) were identified as Candida lusitaniae, Candida catenulate, Candida tropicalis, Candida silvicola and other yeast species, respectively. Only one sample (1.6 %) was identified as Candida albicans by MALDI-TOF however, according to VITEK-2, the agent was not confirmed as C. albicans. According to antifungal susceptibility testing by VITEK-2, one (2.7 %) of the isolates was resistant to fluconazole, one (2.7 %) was resistant to caspofungin, and 4 (10.8 %) were resistant to flucytosine. However, using E test, 10 isolates (27 %) were resistant to flucytosine. Using Rep-PCR, eight genotypic clones were observed. Genotype F (13.8 %) and G (13.8 %; 2 subtypes) were common clones in this study. In conclusion, NAC species were detected in healthy and mastitic cow milk samples. Epidemiological studies need to be conducted to track effectively the main source and to understand the diversity and distribution of the agent. It is necessary to consider the potential risks of yeast contamination in milk for public health. It is essential to focus on adequate sanitation procedures and storage conditions of milk.https://hrcak.srce.hr/file/337367antifungal resistanceMALDI-TOFVITEK-2molecular typingmilknon-albicans Candida (NAC) species |
| spellingShingle | Harun Hizlisoy Nurhan Ertas Onmaz Serhat Al Fulden Karadal Yeliz Yildirim Zafer Gonulalan K. Semih Gumussoy Gonca Demir Aydemir Filiz Kasap Tekinsen Gokcen Dinc Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk Mljekarstvo antifungal resistance MALDI-TOF VITEK-2 molecular typing milk non-albicans Candida (NAC) species |
| title | Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk |
| title_full | Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk |
| title_fullStr | Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk |
| title_full_unstemmed | Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk |
| title_short | Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk |
| title_sort | clonal diversity and antifungal susceptibility of candida spp recovered from cow milk |
| topic | antifungal resistance MALDI-TOF VITEK-2 molecular typing milk non-albicans Candida (NAC) species |
| url | https://hrcak.srce.hr/file/337367 |
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