Chronobiological Changes in Biochemical Hemorrheological Parameters and Cytokine Levels in the Blood of Diabetic Patients on Hemodialysis: A Cross-Sectional Study

<b>Introduction:</b> Diabetes mellitus, a chronic disease characterized by hyperglycemia, is a significant contributor to chronic kidney disease, particularly in patients with diabetic nephropathy undergoing renal replacement therapy. Variations in circadian rhythms can influence the pro...

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Main Authors: Fernando A. Lima, Juliana S. Monção, Mariana S. Honorio, Mahmi Fujimori, Danielle C. H. França, Aron C. M. Cotrim, Emanuelle C. H. França, Aline C. França-Botelho, Danny Laura G. Fagundes-Triches, Patrícia G. F. Marchi, Adenilda C. Honorio-França, Eduardo L. França
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Kidney and Dialysis
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Online Access:https://www.mdpi.com/2673-8236/5/1/9
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author Fernando A. Lima
Juliana S. Monção
Mariana S. Honorio
Mahmi Fujimori
Danielle C. H. França
Aron C. M. Cotrim
Emanuelle C. H. França
Aline C. França-Botelho
Danny Laura G. Fagundes-Triches
Patrícia G. F. Marchi
Adenilda C. Honorio-França
Eduardo L. França
author_facet Fernando A. Lima
Juliana S. Monção
Mariana S. Honorio
Mahmi Fujimori
Danielle C. H. França
Aron C. M. Cotrim
Emanuelle C. H. França
Aline C. França-Botelho
Danny Laura G. Fagundes-Triches
Patrícia G. F. Marchi
Adenilda C. Honorio-França
Eduardo L. França
author_sort Fernando A. Lima
collection DOAJ
description <b>Introduction:</b> Diabetes mellitus, a chronic disease characterized by hyperglycemia, is a significant contributor to chronic kidney disease, particularly in patients with diabetic nephropathy undergoing renal replacement therapy. Variations in circadian rhythms can influence the progression of chronic diseases and treatment outcomes. <b>Aims:</b> This observational study evaluated gender-based chronobiological changes in biochemical, hemorheological factors, and cytokines in patients with dialysis-dependent diabetic nephropathy. <b>Materials and Methods</b>: A cross-sectional study was conducted in Barra do Garças, Brazil, involving 46 patients with type 2 diabetes mellitus who were on regular hemodialysis. Participants were divided into four groups for analysis according to gender and period of day. Inclusion criteria included individuals with type 2 diabetes mellitus undergoing periodic hemodialysis, receiving hemodialysis care at the service, and signing a consent form. Patients with an age under 18 years, diabetes mellitus type 1, or with autoimmune diseases were excluded. Blood samples were collected to assess melatonin, cortisol, biochemical and hemorheological parameters, and cytokines such as IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10, and IL-17. <b>Results</b>: Men exhibited higher melatonin, urea, and creatinine levels in the morning and afternoon phases. At the same time, women showed lower melatonin, increased viscosity, and a decreased deformation rate in the afternoon. Additionally, levels of TNF-α, IFN-γ, and IL-17 were lower in morning serum samples from women. <b>Conclusion:</b> These findings suggest that both gender and circadian timing should be taken into account, when optimizing hemodialysis treatment for patients with diabetic nephropathy. A deeper understanding of these factors could lead to more personalized and effective treatment strategies, ultimately improving patient outcomes and enhancing their quality of life.
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spelling doaj-art-cfd776bfa3194610819d4ec29e9d8be82025-08-20T02:42:35ZengMDPI AGKidney and Dialysis2673-82362025-03-0151910.3390/kidneydial5010009Chronobiological Changes in Biochemical Hemorrheological Parameters and Cytokine Levels in the Blood of Diabetic Patients on Hemodialysis: A Cross-Sectional StudyFernando A. Lima0Juliana S. Monção1Mariana S. Honorio2Mahmi Fujimori3Danielle C. H. França4Aron C. M. Cotrim5Emanuelle C. H. França6Aline C. França-Botelho7Danny Laura G. Fagundes-Triches8Patrícia G. F. Marchi9Adenilda C. Honorio-França10Eduardo L. França11Institute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilFederal Center for Technological Education of Minas Gerais, Araxá 38180-510, MG, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças 78605-091, MT, Brazil<b>Introduction:</b> Diabetes mellitus, a chronic disease characterized by hyperglycemia, is a significant contributor to chronic kidney disease, particularly in patients with diabetic nephropathy undergoing renal replacement therapy. Variations in circadian rhythms can influence the progression of chronic diseases and treatment outcomes. <b>Aims:</b> This observational study evaluated gender-based chronobiological changes in biochemical, hemorheological factors, and cytokines in patients with dialysis-dependent diabetic nephropathy. <b>Materials and Methods</b>: A cross-sectional study was conducted in Barra do Garças, Brazil, involving 46 patients with type 2 diabetes mellitus who were on regular hemodialysis. Participants were divided into four groups for analysis according to gender and period of day. Inclusion criteria included individuals with type 2 diabetes mellitus undergoing periodic hemodialysis, receiving hemodialysis care at the service, and signing a consent form. Patients with an age under 18 years, diabetes mellitus type 1, or with autoimmune diseases were excluded. Blood samples were collected to assess melatonin, cortisol, biochemical and hemorheological parameters, and cytokines such as IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10, and IL-17. <b>Results</b>: Men exhibited higher melatonin, urea, and creatinine levels in the morning and afternoon phases. At the same time, women showed lower melatonin, increased viscosity, and a decreased deformation rate in the afternoon. Additionally, levels of TNF-α, IFN-γ, and IL-17 were lower in morning serum samples from women. <b>Conclusion:</b> These findings suggest that both gender and circadian timing should be taken into account, when optimizing hemodialysis treatment for patients with diabetic nephropathy. A deeper understanding of these factors could lead to more personalized and effective treatment strategies, ultimately improving patient outcomes and enhancing their quality of life.https://www.mdpi.com/2673-8236/5/1/9diabetes mellitusdiabetic nephropathycytokineschronic renal diseaserheology
spellingShingle Fernando A. Lima
Juliana S. Monção
Mariana S. Honorio
Mahmi Fujimori
Danielle C. H. França
Aron C. M. Cotrim
Emanuelle C. H. França
Aline C. França-Botelho
Danny Laura G. Fagundes-Triches
Patrícia G. F. Marchi
Adenilda C. Honorio-França
Eduardo L. França
Chronobiological Changes in Biochemical Hemorrheological Parameters and Cytokine Levels in the Blood of Diabetic Patients on Hemodialysis: A Cross-Sectional Study
Kidney and Dialysis
diabetes mellitus
diabetic nephropathy
cytokines
chronic renal disease
rheology
title Chronobiological Changes in Biochemical Hemorrheological Parameters and Cytokine Levels in the Blood of Diabetic Patients on Hemodialysis: A Cross-Sectional Study
title_full Chronobiological Changes in Biochemical Hemorrheological Parameters and Cytokine Levels in the Blood of Diabetic Patients on Hemodialysis: A Cross-Sectional Study
title_fullStr Chronobiological Changes in Biochemical Hemorrheological Parameters and Cytokine Levels in the Blood of Diabetic Patients on Hemodialysis: A Cross-Sectional Study
title_full_unstemmed Chronobiological Changes in Biochemical Hemorrheological Parameters and Cytokine Levels in the Blood of Diabetic Patients on Hemodialysis: A Cross-Sectional Study
title_short Chronobiological Changes in Biochemical Hemorrheological Parameters and Cytokine Levels in the Blood of Diabetic Patients on Hemodialysis: A Cross-Sectional Study
title_sort chronobiological changes in biochemical hemorrheological parameters and cytokine levels in the blood of diabetic patients on hemodialysis a cross sectional study
topic diabetes mellitus
diabetic nephropathy
cytokines
chronic renal disease
rheology
url https://www.mdpi.com/2673-8236/5/1/9
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