Polymerized laminin-modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretion
Encapsulation of human pancreatic islets in semipermeable membranes offers a promising, less invasive alternative to whole pancreas transplantation for Type 1 Diabetes (T1D), potentially reducing the need for immunosuppressive drugs and improving graft survival. However, the inflammatory environment...
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Elsevier
2025-06-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425003722 |
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| author | Isaura Beatriz Borges Silva Theo Borghuis Tian Qin Mari Cleide Sogayar Paul de Vos |
| author_facet | Isaura Beatriz Borges Silva Theo Borghuis Tian Qin Mari Cleide Sogayar Paul de Vos |
| author_sort | Isaura Beatriz Borges Silva |
| collection | DOAJ |
| description | Encapsulation of human pancreatic islets in semipermeable membranes offers a promising, less invasive alternative to whole pancreas transplantation for Type 1 Diabetes (T1D), potentially reducing the need for immunosuppressive drugs and improving graft survival. However, the inflammatory environment during transplantation poses significant challenges, often leading to substantial graft loss. This study explores modifying the intracapsular environment with extracellular matrix (ECM) molecules – chondroitin sulfate (ChS), laminin (LN), and polymerized laminin (pLN) – to enhance islet cell resilience against cytokine-induced stress, associated with the post-transplantation environment. Encapsulated mouse insulinoma β-cells (MIN6) and human islets were exposed to pro-inflammatory cytokines (IL-1β, IFN-γ, TNF-α) over 1, 3, and 5 days. The results showed that LN and pLN in combination with ChS particularly improved outcomes, with LN reducing oxidative stress and pLN significantly lowering cell death and pro-inflammatory chemokine MCP-1 production. These findings highlight the potential of ECM-modified encapsulation to enhance the survival of transplanted islets, offering a more favorable approach for T1D treatment and allowing transplantation of pancreatic islets with fewer islets. |
| format | Article |
| id | doaj-art-cfcab2795d0a4e709cadf5fd3e3dedef |
| institution | DOAJ |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-cfcab2795d0a4e709cadf5fd3e3dedef2025-08-20T03:09:12ZengElsevierMaterials Today Bio2590-00642025-06-013210181210.1016/j.mtbio.2025.101812Polymerized laminin-modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretionIsaura Beatriz Borges Silva0Theo Borghuis1Tian Qin2Mari Cleide Sogayar3Paul de Vos4Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands; Cell and Molecular Therapy NUCEL Group, School of Medicine, University of São Paulo, São Paulo, Brazil; Department of Biochemistry, Chemistry Institute, University of São Paulo, Brazil; Corresponding author. University of Groningen, University Medical Center Groningen, Hanzeplein 1, EA11, 9713 GZ Groningen, The Netherlands.Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The NetherlandsDepartment of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The NetherlandsCell and Molecular Therapy NUCEL Group, School of Medicine, University of São Paulo, São Paulo, Brazil; Department of Biochemistry, Chemistry Institute, University of São Paulo, BrazilDepartment of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The NetherlandsEncapsulation of human pancreatic islets in semipermeable membranes offers a promising, less invasive alternative to whole pancreas transplantation for Type 1 Diabetes (T1D), potentially reducing the need for immunosuppressive drugs and improving graft survival. However, the inflammatory environment during transplantation poses significant challenges, often leading to substantial graft loss. This study explores modifying the intracapsular environment with extracellular matrix (ECM) molecules – chondroitin sulfate (ChS), laminin (LN), and polymerized laminin (pLN) – to enhance islet cell resilience against cytokine-induced stress, associated with the post-transplantation environment. Encapsulated mouse insulinoma β-cells (MIN6) and human islets were exposed to pro-inflammatory cytokines (IL-1β, IFN-γ, TNF-α) over 1, 3, and 5 days. The results showed that LN and pLN in combination with ChS particularly improved outcomes, with LN reducing oxidative stress and pLN significantly lowering cell death and pro-inflammatory chemokine MCP-1 production. These findings highlight the potential of ECM-modified encapsulation to enhance the survival of transplanted islets, offering a more favorable approach for T1D treatment and allowing transplantation of pancreatic islets with fewer islets.http://www.sciencedirect.com/science/article/pii/S2590006425003722EncapsulationType 1 DiabetesExtracellular matrixIslet transplantationLaminin |
| spellingShingle | Isaura Beatriz Borges Silva Theo Borghuis Tian Qin Mari Cleide Sogayar Paul de Vos Polymerized laminin-modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretion Materials Today Bio Encapsulation Type 1 Diabetes Extracellular matrix Islet transplantation Laminin |
| title | Polymerized laminin-modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretion |
| title_full | Polymerized laminin-modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretion |
| title_fullStr | Polymerized laminin-modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretion |
| title_full_unstemmed | Polymerized laminin-modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretion |
| title_short | Polymerized laminin-modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretion |
| title_sort | polymerized laminin modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretion |
| topic | Encapsulation Type 1 Diabetes Extracellular matrix Islet transplantation Laminin |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425003722 |
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