Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original Research
The persistence of human immunodeficiency virus (HIV) within viral reservoirs poses significant challenges to eradication efforts. Epigenetic alterations, including DNA methylation, are potential factors influencing the latency and persistence of HIV. This study details the development and applicati...
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2025-03-01
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| Online Access: | https://www.mdpi.com/1999-4915/17/4/465 |
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| author | Anna Esman Svetlana Salamaikina Alina Kirichenko Michael Vinokurov Darya Fomina Kirill Sikamov Arina Syrkina Anastasia Pokrovskaya Vasily Akimkin |
| author_facet | Anna Esman Svetlana Salamaikina Alina Kirichenko Michael Vinokurov Darya Fomina Kirill Sikamov Arina Syrkina Anastasia Pokrovskaya Vasily Akimkin |
| author_sort | Anna Esman |
| collection | DOAJ |
| description | The persistence of human immunodeficiency virus (HIV) within viral reservoirs poses significant challenges to eradication efforts. Epigenetic alterations, including DNA methylation, are potential factors influencing the latency and persistence of HIV. This study details the development and application of techniques to assess CpG methylation in the promoter regions of the <i>CCR5</i> and <i>CXCR4</i> genes, which are key HIV-1 coreceptors. Using both Sanger sequencing and pyrosequencing methods, we examined 51 biological samples from 17 people living with HIV at three time points: baseline (week 0) and post-antiretroviral therapy (ART) at weeks 24 and 48. Our results revealed that <i>CXCR4</i> promoter CpG sites were largely unmethylated, while <i>CCR5</i> promoter CpGs exhibited significant variability in methylation levels. Specifically, <i>CCR5</i> CpG 1 showed a significant decrease in methylation from week 0 to week 48, while <i>CXCR4</i> CpG 3 displayed a significant decrease between week 0 and week 24. These differences were statistically significant when compared with non-HIV-infected controls. These findings demonstrate distinct methylation patterns between <i>CCR5</i> and <i>CXCR4</i> promoters in people living with HIV over time, suggesting that epigenetic modifications may play a role in regulating the persistence of HIV-1. Our techniques provide a reliable framework for assessing gene promoter methylation and could be applied in further research on the epigenetics of HIV. |
| format | Article |
| id | doaj-art-cfc8f258aa944babbd44788906ca789d |
| institution | OA Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-cfc8f258aa944babbd44788906ca789d2025-08-20T02:18:01ZengMDPI AGViruses1999-49152025-03-0117446510.3390/v17040465Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original ResearchAnna Esman0Svetlana Salamaikina1Alina Kirichenko2Michael Vinokurov3Darya Fomina4Kirill Sikamov5Arina Syrkina6Anastasia Pokrovskaya7Vasily Akimkin8Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Central Research Institute of Epidemiology, 111123 Moscow, RussiaFederal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Central Research Institute of Epidemiology, 111123 Moscow, RussiaFederal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Central Research Institute of Epidemiology, 111123 Moscow, RussiaFederal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Central Research Institute of Epidemiology, 111123 Moscow, RussiaFederal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Central Research Institute of Epidemiology, 111123 Moscow, RussiaFederal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Central Research Institute of Epidemiology, 111123 Moscow, RussiaFederal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Central Research Institute of Epidemiology, 111123 Moscow, RussiaFederal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Central Research Institute of Epidemiology, 111123 Moscow, RussiaFederal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Central Research Institute of Epidemiology, 111123 Moscow, RussiaThe persistence of human immunodeficiency virus (HIV) within viral reservoirs poses significant challenges to eradication efforts. Epigenetic alterations, including DNA methylation, are potential factors influencing the latency and persistence of HIV. This study details the development and application of techniques to assess CpG methylation in the promoter regions of the <i>CCR5</i> and <i>CXCR4</i> genes, which are key HIV-1 coreceptors. Using both Sanger sequencing and pyrosequencing methods, we examined 51 biological samples from 17 people living with HIV at three time points: baseline (week 0) and post-antiretroviral therapy (ART) at weeks 24 and 48. Our results revealed that <i>CXCR4</i> promoter CpG sites were largely unmethylated, while <i>CCR5</i> promoter CpGs exhibited significant variability in methylation levels. Specifically, <i>CCR5</i> CpG 1 showed a significant decrease in methylation from week 0 to week 48, while <i>CXCR4</i> CpG 3 displayed a significant decrease between week 0 and week 24. These differences were statistically significant when compared with non-HIV-infected controls. These findings demonstrate distinct methylation patterns between <i>CCR5</i> and <i>CXCR4</i> promoters in people living with HIV over time, suggesting that epigenetic modifications may play a role in regulating the persistence of HIV-1. Our techniques provide a reliable framework for assessing gene promoter methylation and could be applied in further research on the epigenetics of HIV.https://www.mdpi.com/1999-4915/17/4/465epigeneticHIV infectionsCpGmethylationHIV coreceptors<i>CCR5</i> |
| spellingShingle | Anna Esman Svetlana Salamaikina Alina Kirichenko Michael Vinokurov Darya Fomina Kirill Sikamov Arina Syrkina Anastasia Pokrovskaya Vasily Akimkin Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original Research Viruses epigenetic HIV infections CpG methylation HIV coreceptors <i>CCR5</i> |
| title | Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original Research |
| title_full | Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original Research |
| title_fullStr | Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original Research |
| title_full_unstemmed | Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original Research |
| title_short | Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original Research |
| title_sort | promoter methylation of hiv coreceptor related genes ccr5 and cxcr4 original research |
| topic | epigenetic HIV infections CpG methylation HIV coreceptors <i>CCR5</i> |
| url | https://www.mdpi.com/1999-4915/17/4/465 |
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