Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor.
<h4>Background</h4>Health assessment of the transplanted organ is very important due to the relationship of long-term survival of organ transplant recipients and health organ maintenance. Nowadays, the measurement of cell-free DNA from grafts in the circulation of transplant recipients h...
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0113987&type=printable |
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| author | Hada C Macher Gonzalo Suárez-Artacho Juan M Guerrero Miguel A Gómez-Bravo Sara Álvarez-Gómez Carmen Bernal-Bellido Inmaculada Dominguez-Pascual Amalia Rubio |
| author_facet | Hada C Macher Gonzalo Suárez-Artacho Juan M Guerrero Miguel A Gómez-Bravo Sara Álvarez-Gómez Carmen Bernal-Bellido Inmaculada Dominguez-Pascual Amalia Rubio |
| author_sort | Hada C Macher |
| collection | DOAJ |
| description | <h4>Background</h4>Health assessment of the transplanted organ is very important due to the relationship of long-term survival of organ transplant recipients and health organ maintenance. Nowadays, the measurement of cell-free DNA from grafts in the circulation of transplant recipients has been considered a potential biomarker of organ rejection or transplant associated complications in an attempt to replace or reduce liver biopsy. However, methods developed to date are expensive and extremely time-consuming. Our approach was to measure the SRY gene, as a male organ biomarker, in a setting of sex-mismatched female recipients of male donor organs.<h4>Methods</h4>Cell-free DNA quantization of the SRY gene was performed by real-time quantitative PCR beforehand, at the moment of transplantation during reperfusion (day 0) and during the stay at the intensive care unit. Beta-globin cell-free DNA levels, a general cellular damage marker, were also quantified.<h4>Results</h4>Beta-globin mean values of patients, who accepted the graft without any complications during the first week after surgery, diminished from day 0 until patient stabilization. This decrease was not so evident in patients who suffered some kind of post-transplantation complications. All patients showed an increase in SRY levels at day 0, which decreased during hospitalization. Different complications that did not compromise donated organs showed increased beta-globin levels but no SRY gene levels. However, when a donated organ was damaged the patients exhibited high levels of both genes.<h4>Conclusion</h4>Determination of a SRY gene in a female recipient's serum is a clear and specific biomarker of donated organs and may give us important information about graft health in a short period of time by a non-expensive technique. This approach may permit clinicians to maintain a close follow up of the transplanted patient. |
| format | Article |
| id | doaj-art-cfbd41727b104cfaac5b0a57fc5847de |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-cfbd41727b104cfaac5b0a57fc5847de2025-08-20T02:15:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11398710.1371/journal.pone.0113987Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor.Hada C MacherGonzalo Suárez-ArtachoJuan M GuerreroMiguel A Gómez-BravoSara Álvarez-GómezCarmen Bernal-BellidoInmaculada Dominguez-PascualAmalia Rubio<h4>Background</h4>Health assessment of the transplanted organ is very important due to the relationship of long-term survival of organ transplant recipients and health organ maintenance. Nowadays, the measurement of cell-free DNA from grafts in the circulation of transplant recipients has been considered a potential biomarker of organ rejection or transplant associated complications in an attempt to replace or reduce liver biopsy. However, methods developed to date are expensive and extremely time-consuming. Our approach was to measure the SRY gene, as a male organ biomarker, in a setting of sex-mismatched female recipients of male donor organs.<h4>Methods</h4>Cell-free DNA quantization of the SRY gene was performed by real-time quantitative PCR beforehand, at the moment of transplantation during reperfusion (day 0) and during the stay at the intensive care unit. Beta-globin cell-free DNA levels, a general cellular damage marker, were also quantified.<h4>Results</h4>Beta-globin mean values of patients, who accepted the graft without any complications during the first week after surgery, diminished from day 0 until patient stabilization. This decrease was not so evident in patients who suffered some kind of post-transplantation complications. All patients showed an increase in SRY levels at day 0, which decreased during hospitalization. Different complications that did not compromise donated organs showed increased beta-globin levels but no SRY gene levels. However, when a donated organ was damaged the patients exhibited high levels of both genes.<h4>Conclusion</h4>Determination of a SRY gene in a female recipient's serum is a clear and specific biomarker of donated organs and may give us important information about graft health in a short period of time by a non-expensive technique. This approach may permit clinicians to maintain a close follow up of the transplanted patient.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0113987&type=printable |
| spellingShingle | Hada C Macher Gonzalo Suárez-Artacho Juan M Guerrero Miguel A Gómez-Bravo Sara Álvarez-Gómez Carmen Bernal-Bellido Inmaculada Dominguez-Pascual Amalia Rubio Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor. PLoS ONE |
| title | Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor. |
| title_full | Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor. |
| title_fullStr | Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor. |
| title_full_unstemmed | Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor. |
| title_short | Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor. |
| title_sort | monitoring of transplanted liver health by quantification of organ specific genomic marker in circulating dna from receptor |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0113987&type=printable |
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