Oral Administration of Lactobacillus Inhibits the Permeability of Blood-Brain and Gut Barriers in a Parkinsonism Model

It has recently been shown that the administration of probiotics can modulate the microbiota-gut-brain axis and may have favorable effects in models of Parkinson’s disease. In this study, we used a hemiparkinsonism model induced by the neurotoxin 6-OHDA to evaluate the efficacy of the administration...

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Main Authors: Angélica Y. Nápoles-Medina, Blanca R. Aguilar-Uscanga, Josué R. Solís-Pacheco, Aldo R. Tejeda-Martínez, Luis J. Ramírez-Jirano, María F. Urmeneta-Ortiz, Veronica Chaparro-Huerta, Mario E. Flores-Soto
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:Behavioural Neurology
Online Access:http://dx.doi.org/10.1155/2023/6686037
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author Angélica Y. Nápoles-Medina
Blanca R. Aguilar-Uscanga
Josué R. Solís-Pacheco
Aldo R. Tejeda-Martínez
Luis J. Ramírez-Jirano
María F. Urmeneta-Ortiz
Veronica Chaparro-Huerta
Mario E. Flores-Soto
author_facet Angélica Y. Nápoles-Medina
Blanca R. Aguilar-Uscanga
Josué R. Solís-Pacheco
Aldo R. Tejeda-Martínez
Luis J. Ramírez-Jirano
María F. Urmeneta-Ortiz
Veronica Chaparro-Huerta
Mario E. Flores-Soto
author_sort Angélica Y. Nápoles-Medina
collection DOAJ
description It has recently been shown that the administration of probiotics can modulate the microbiota-gut-brain axis and may have favorable effects in models of Parkinson’s disease. In this study, we used a hemiparkinsonism model induced by the neurotoxin 6-OHDA to evaluate the efficacy of the administration of a four-week administration of a mixture containing the microorganisms Lactobacillus fermentum LH01, Lactobacillus reuteri LH03, and Lactobacillus plantarum LH05. The hemiparkinsonism model induced an increase in rotations in the apomorphine test, along with a decrease in the latency time to fall in the rotarod test on days 14 and 21 after surgery, respectively. The administration of probiotics was sufficient to improve this condition. The model also showed a decrease in tyrosine hydroxylase immunoreactivity in the striatum and the number of labeled cells in the substantia nigra, both of which were counteracted by the administration of probiotics. The permeability of the blood-brain barrier was increased in the model, but this effect was reversed by the probiotics for both brain regions. The gut barrier was permeated with the model, and this effect was reversed and dropped to lower levels than the control group after the administration of probiotics. Finally, lipid peroxidation showed a pattern of differences similar to that of permeabilities. The inhibition of the permeability of the blood-brain and gut barriers mediated by the administration of probiotics will likely provide protection by downregulating oxidative stress, thus affecting the rotarod test performance.
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spelling doaj-art-cfb3e9cf31ef4817b8900ff70c23acc72025-08-20T02:20:06ZengWileyBehavioural Neurology1875-85842023-01-01202310.1155/2023/6686037Oral Administration of Lactobacillus Inhibits the Permeability of Blood-Brain and Gut Barriers in a Parkinsonism ModelAngélica Y. Nápoles-Medina0Blanca R. Aguilar-Uscanga1Josué R. Solís-Pacheco2Aldo R. Tejeda-Martínez3Luis J. Ramírez-Jirano4María F. Urmeneta-Ortiz5Veronica Chaparro-Huerta6Mario E. Flores-Soto7Laboratorio de Neurobiología Celular y MolecularDepartamento de FarmacobiologíaDepartamento de FarmacobiologíaLaboratorio de Neurobiología Celular y MolecularDivisión de NeurocienciasLaboratorio de Neurobiología Celular y MolecularLaboratorio de Neurobiología Celular y MolecularLaboratorio de Neurobiología Celular y MolecularIt has recently been shown that the administration of probiotics can modulate the microbiota-gut-brain axis and may have favorable effects in models of Parkinson’s disease. In this study, we used a hemiparkinsonism model induced by the neurotoxin 6-OHDA to evaluate the efficacy of the administration of a four-week administration of a mixture containing the microorganisms Lactobacillus fermentum LH01, Lactobacillus reuteri LH03, and Lactobacillus plantarum LH05. The hemiparkinsonism model induced an increase in rotations in the apomorphine test, along with a decrease in the latency time to fall in the rotarod test on days 14 and 21 after surgery, respectively. The administration of probiotics was sufficient to improve this condition. The model also showed a decrease in tyrosine hydroxylase immunoreactivity in the striatum and the number of labeled cells in the substantia nigra, both of which were counteracted by the administration of probiotics. The permeability of the blood-brain barrier was increased in the model, but this effect was reversed by the probiotics for both brain regions. The gut barrier was permeated with the model, and this effect was reversed and dropped to lower levels than the control group after the administration of probiotics. Finally, lipid peroxidation showed a pattern of differences similar to that of permeabilities. The inhibition of the permeability of the blood-brain and gut barriers mediated by the administration of probiotics will likely provide protection by downregulating oxidative stress, thus affecting the rotarod test performance.http://dx.doi.org/10.1155/2023/6686037
spellingShingle Angélica Y. Nápoles-Medina
Blanca R. Aguilar-Uscanga
Josué R. Solís-Pacheco
Aldo R. Tejeda-Martínez
Luis J. Ramírez-Jirano
María F. Urmeneta-Ortiz
Veronica Chaparro-Huerta
Mario E. Flores-Soto
Oral Administration of Lactobacillus Inhibits the Permeability of Blood-Brain and Gut Barriers in a Parkinsonism Model
Behavioural Neurology
title Oral Administration of Lactobacillus Inhibits the Permeability of Blood-Brain and Gut Barriers in a Parkinsonism Model
title_full Oral Administration of Lactobacillus Inhibits the Permeability of Blood-Brain and Gut Barriers in a Parkinsonism Model
title_fullStr Oral Administration of Lactobacillus Inhibits the Permeability of Blood-Brain and Gut Barriers in a Parkinsonism Model
title_full_unstemmed Oral Administration of Lactobacillus Inhibits the Permeability of Blood-Brain and Gut Barriers in a Parkinsonism Model
title_short Oral Administration of Lactobacillus Inhibits the Permeability of Blood-Brain and Gut Barriers in a Parkinsonism Model
title_sort oral administration of lactobacillus inhibits the permeability of blood brain and gut barriers in a parkinsonism model
url http://dx.doi.org/10.1155/2023/6686037
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