Identification and expression analysis of the FRK gene family in Oilseed (Brassica napus L.)
Abstract Fructokinases (FRKs) are pivotal enzymes involved in sugar metabolism and plant growth regulation. In this study, a total of 38 BnFRK genes were identified in the Brassica napus genome through a comprehensive genome-wide analysis. Phylogenetic classification divided these genes into four gr...
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BMC
2025-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06964-2 |
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| author | Rui Yang Jindong Chen Ying Huang Lingli Xie Jinsong Xu Le Xu Xuekun Zhang Benbo Xu |
| author_facet | Rui Yang Jindong Chen Ying Huang Lingli Xie Jinsong Xu Le Xu Xuekun Zhang Benbo Xu |
| author_sort | Rui Yang |
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| description | Abstract Fructokinases (FRKs) are pivotal enzymes involved in sugar metabolism and plant growth regulation. In this study, a total of 38 BnFRK genes were identified in the Brassica napus genome through a comprehensive genome-wide analysis. Phylogenetic classification divided these genes into four groups, with conserved motif and gene structure analyses indicating both functional conservation and divergence. Synteny and collinearity analyses revealed extensive gene duplication events, particularly within Group IV, suggesting lineage-specific expansion after polyploidization. Promoter cis-element analysis indicated that BnFRKs are potentially involved in hormone response and abiotic stress regulation. Transcriptome data and qRT-PCR validation demonstrated that several BnFRKs, especially BnFRK12, showed significant induction under salt, alkali, low nitrogen and osmotic stress conditions, highlighting their potential roles in stress adaptation. These findings provide a foundation for further functional characterization of BnFRK genes and their application in improving stress resilience in rapeseed. |
| format | Article |
| id | doaj-art-cfa9261dd1a94245a03f9fc787fa5989 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-cfa9261dd1a94245a03f9fc787fa59892025-08-20T04:01:56ZengBMCBMC Plant Biology1471-22292025-07-0125111410.1186/s12870-025-06964-2Identification and expression analysis of the FRK gene family in Oilseed (Brassica napus L.)Rui Yang0Jindong Chen1Ying Huang2Lingli Xie3Jinsong Xu4Le Xu5Xuekun Zhang6Benbo Xu7MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze UniversityMARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze UniversityMARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze UniversityMARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze UniversityMARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze UniversityMARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze UniversityMARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze UniversityMARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), College of Agriculture, Yangtze UniversityAbstract Fructokinases (FRKs) are pivotal enzymes involved in sugar metabolism and plant growth regulation. In this study, a total of 38 BnFRK genes were identified in the Brassica napus genome through a comprehensive genome-wide analysis. Phylogenetic classification divided these genes into four groups, with conserved motif and gene structure analyses indicating both functional conservation and divergence. Synteny and collinearity analyses revealed extensive gene duplication events, particularly within Group IV, suggesting lineage-specific expansion after polyploidization. Promoter cis-element analysis indicated that BnFRKs are potentially involved in hormone response and abiotic stress regulation. Transcriptome data and qRT-PCR validation demonstrated that several BnFRKs, especially BnFRK12, showed significant induction under salt, alkali, low nitrogen and osmotic stress conditions, highlighting their potential roles in stress adaptation. These findings provide a foundation for further functional characterization of BnFRK genes and their application in improving stress resilience in rapeseed.https://doi.org/10.1186/s12870-025-06964-2Brassica napusFRK gene familyAbiotic stressGene expression |
| spellingShingle | Rui Yang Jindong Chen Ying Huang Lingli Xie Jinsong Xu Le Xu Xuekun Zhang Benbo Xu Identification and expression analysis of the FRK gene family in Oilseed (Brassica napus L.) BMC Plant Biology Brassica napus FRK gene family Abiotic stress Gene expression |
| title | Identification and expression analysis of the FRK gene family in Oilseed (Brassica napus L.) |
| title_full | Identification and expression analysis of the FRK gene family in Oilseed (Brassica napus L.) |
| title_fullStr | Identification and expression analysis of the FRK gene family in Oilseed (Brassica napus L.) |
| title_full_unstemmed | Identification and expression analysis of the FRK gene family in Oilseed (Brassica napus L.) |
| title_short | Identification and expression analysis of the FRK gene family in Oilseed (Brassica napus L.) |
| title_sort | identification and expression analysis of the frk gene family in oilseed brassica napus l |
| topic | Brassica napus FRK gene family Abiotic stress Gene expression |
| url | https://doi.org/10.1186/s12870-025-06964-2 |
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