MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression
We attempted to analyze the effects of miR-1-3p and CCL2 on the proliferation, migration, and invasion of bladder cancer cells. A total of 18 pairs of bladder cancer tissues with corresponding adjacent tissues and the 6 cases of normal tissues were collected. The expressions of miR-1-3p and CCL2 in...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-05-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317698383 |
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| _version_ | 1850055878226477056 |
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| author | Weiwei Wang Fujun Shen Chunlei Wang Wenying Lu Jun Wei Anquan Shang Chunbin Wang |
| author_facet | Weiwei Wang Fujun Shen Chunlei Wang Wenying Lu Jun Wei Anquan Shang Chunbin Wang |
| author_sort | Weiwei Wang |
| collection | DOAJ |
| description | We attempted to analyze the effects of miR-1-3p and CCL2 on the proliferation, migration, and invasion of bladder cancer cells. A total of 18 pairs of bladder cancer tissues with corresponding adjacent tissues and the 6 cases of normal tissues were collected. The expressions of miR-1-3p and CCL2 in the cancer tissues were evaluated using quantitative real-time polymerase chain reaction and western blot. The relationship between miR-1-3p and CCL2 was assessed using luciferase reporter assay. The UM-UC-3 bladder cancer cells were transfected with CCL2 small interfering RNA and miR-1-3p mimics. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, wound healing assay, Transwell assay, and the flow cytometry test were used to detect the proliferation, migration, invasion, and apoptosis of bladder cancer cells. Bladder cancer tissues had lower levels of miR-1-3p but higher levels of CCL2 than normal tissues ( p < 0.05). The transfection of miR-1-3p mimics and CCL2 small interfering RNA remarkably suppressed cell proliferation and invasion and promoted apoptosis of cells ( p < 0.05). Results of the luciferase reporter gene assay demonstrated that miR-1-3p targeted CCL2. MiR-1-3p suppresses the proliferation and invasion of urinary bladder cancer cells by targeting CCL2. |
| format | Article |
| id | doaj-art-cfa32a2224ef485c8116d4fb73fe3440 |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-cfa32a2224ef485c8116d4fb73fe34402025-08-20T02:51:50ZengSAGE PublishingTumor Biology1423-03802017-05-013910.1177/1010428317698383MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expressionWeiwei Wang0Fujun Shen1Chunlei Wang2Wenying Lu3Jun Wei4Anquan Shang5Chunbin Wang6Department of Pathology, The Sixth People’s Hospital of Yancheng City, Yancheng, ChinaDepartment of Oncology, Yancheng Hospital Affiliated to Medical College of Southeast University and The Third People’s Hospital of Yancheng City, Yancheng, ChinaDepartment of Laboratory Medicine, The Sixth People’s Hospital of Yancheng City, Yancheng, ChinaDepartment of Laboratory Medicine, The Sixth People’s Hospital of Yancheng City, Yancheng, ChinaClinical Medicine School, Ningxia Medical University, Yinchuan, ChinaClinical Medicine School, Ningxia Medical University, Yinchuan, ChinaDepartment of Oncology, Yancheng Hospital Affiliated to Medical College of Southeast University and The Third People’s Hospital of Yancheng City, Yancheng, ChinaWe attempted to analyze the effects of miR-1-3p and CCL2 on the proliferation, migration, and invasion of bladder cancer cells. A total of 18 pairs of bladder cancer tissues with corresponding adjacent tissues and the 6 cases of normal tissues were collected. The expressions of miR-1-3p and CCL2 in the cancer tissues were evaluated using quantitative real-time polymerase chain reaction and western blot. The relationship between miR-1-3p and CCL2 was assessed using luciferase reporter assay. The UM-UC-3 bladder cancer cells were transfected with CCL2 small interfering RNA and miR-1-3p mimics. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, wound healing assay, Transwell assay, and the flow cytometry test were used to detect the proliferation, migration, invasion, and apoptosis of bladder cancer cells. Bladder cancer tissues had lower levels of miR-1-3p but higher levels of CCL2 than normal tissues ( p < 0.05). The transfection of miR-1-3p mimics and CCL2 small interfering RNA remarkably suppressed cell proliferation and invasion and promoted apoptosis of cells ( p < 0.05). Results of the luciferase reporter gene assay demonstrated that miR-1-3p targeted CCL2. MiR-1-3p suppresses the proliferation and invasion of urinary bladder cancer cells by targeting CCL2.https://doi.org/10.1177/1010428317698383 |
| spellingShingle | Weiwei Wang Fujun Shen Chunlei Wang Wenying Lu Jun Wei Anquan Shang Chunbin Wang MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression Tumor Biology |
| title | MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression |
| title_full | MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression |
| title_fullStr | MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression |
| title_full_unstemmed | MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression |
| title_short | MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression |
| title_sort | mir 1 3p inhibits the proliferation and invasion of bladder cancer cells by suppressing ccl2 expression |
| url | https://doi.org/10.1177/1010428317698383 |
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