Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study
Three-phase partitioning (TPP) is promising for isolating bioactive polysaccharides, but t-butanol’s environmental impact limits its application. Deep eutectic solvents can serve as a green and recyclable alternative to t-butanol. This study introduces an ultrasonic-assisted DES three-phase partitio...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417724004553 |
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| author | Zhongnan Wu Chong Li Junhao Li Tanggan Wang Meifeng Li Leyi Zhao Huimei Ye Jiaheng Chen Jiajia Zan Lijun Song Qian Zhang Shaojie Zhang |
| author_facet | Zhongnan Wu Chong Li Junhao Li Tanggan Wang Meifeng Li Leyi Zhao Huimei Ye Jiaheng Chen Jiajia Zan Lijun Song Qian Zhang Shaojie Zhang |
| author_sort | Zhongnan Wu |
| collection | DOAJ |
| description | Three-phase partitioning (TPP) is promising for isolating bioactive polysaccharides, but t-butanol’s environmental impact limits its application. Deep eutectic solvents can serve as a green and recyclable alternative to t-butanol. This study introduces an ultrasonic-assisted DES three-phase partitioning (UA-TPP-DES) system to extract and purify American ginseng polysaccharides (AGPs). The optimized DES-4, composed of lauric acid and nonanoic acid (molar ratio = 1:1), achieved a 35.28 % extraction yield under specific conditions and can be recycled five times with minimal yield loss. AGP-DES-4 has a broad molecular weight distribution (2.48–174.64 kDa) and mainly consists of mannose, glucose, galactose, and arabinose. In vivo Drosophila models show that AGP-DES-4 improves UC fly survival and enhances intestinal barrier function by regulating the proliferation and differentiation of intestinal epithelial cells (IECs) and intestinal stem cells (ISCs). Our results highlight the effectiveness of the AGP-DES-4 extraction method and its potential therapeutic value for treating UC. |
| format | Article |
| id | doaj-art-cf9dbfa8da8b4243ba06dac098a2fb11 |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-cf9dbfa8da8b4243ba06dac098a2fb112025-01-11T06:38:50ZengElsevierUltrasonics Sonochemistry1350-41772025-01-01112107206Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis studyZhongnan Wu0Chong Li1Junhao Li2Tanggan Wang3Meifeng Li4Leyi Zhao5Huimei Ye6Jiaheng Chen7Jiajia Zan8Lijun Song9Qian Zhang10Shaojie Zhang11The Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808, ChinaCollege of Life Sciences, Shandong Agricultural University, Tai’an 271018, ChinaThe Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808, ChinaThe Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808, ChinaThe Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808, ChinaSchool of Pharmacy, Guangdong Provincial Key Laboratory of Advanced Drug Delivery, Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Pharmacy, Guangdong Provincial Key Laboratory of Advanced Drug Delivery, Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaCAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, ChinaSchool of Pharmacy, Guangdong Provincial Key Laboratory of Advanced Drug Delivery, Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaThe Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808, China; Corresponding authors.School of Pharmacy, Guangdong Provincial Key Laboratory of Advanced Drug Delivery, Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou 510006, China; Corresponding authors.The Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808, China; Corresponding authors.Three-phase partitioning (TPP) is promising for isolating bioactive polysaccharides, but t-butanol’s environmental impact limits its application. Deep eutectic solvents can serve as a green and recyclable alternative to t-butanol. This study introduces an ultrasonic-assisted DES three-phase partitioning (UA-TPP-DES) system to extract and purify American ginseng polysaccharides (AGPs). The optimized DES-4, composed of lauric acid and nonanoic acid (molar ratio = 1:1), achieved a 35.28 % extraction yield under specific conditions and can be recycled five times with minimal yield loss. AGP-DES-4 has a broad molecular weight distribution (2.48–174.64 kDa) and mainly consists of mannose, glucose, galactose, and arabinose. In vivo Drosophila models show that AGP-DES-4 improves UC fly survival and enhances intestinal barrier function by regulating the proliferation and differentiation of intestinal epithelial cells (IECs) and intestinal stem cells (ISCs). Our results highlight the effectiveness of the AGP-DES-4 extraction method and its potential therapeutic value for treating UC.http://www.sciencedirect.com/science/article/pii/S1350417724004553Deep eutectic solventsUltrasonicAmerican ginsengPolysaccharideDrosophilaAnti-ulcerative colitis activity |
| spellingShingle | Zhongnan Wu Chong Li Junhao Li Tanggan Wang Meifeng Li Leyi Zhao Huimei Ye Jiaheng Chen Jiajia Zan Lijun Song Qian Zhang Shaojie Zhang Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study Ultrasonics Sonochemistry Deep eutectic solvents Ultrasonic American ginseng Polysaccharide Drosophila Anti-ulcerative colitis activity |
| title | Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study |
| title_full | Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study |
| title_fullStr | Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study |
| title_full_unstemmed | Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study |
| title_short | Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study |
| title_sort | extraction of american ginseng polysaccharide by ultrasound assisted deep eutectic solvents based three phase partitioning process optimization structural characterization and anti ulcerative colitis study |
| topic | Deep eutectic solvents Ultrasonic American ginseng Polysaccharide Drosophila Anti-ulcerative colitis activity |
| url | http://www.sciencedirect.com/science/article/pii/S1350417724004553 |
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