Determination of Atropine Sulfate in Human Urines by Capillary Electrophoresis Using Chemical Modified Electrode as Electrochemiluminescence Sensor
A Ru(bpy)3 2+-based electrochemiluminescence (ECL) detection coupled with capillary electrophoresis (CE) was developed for the determination of atropine sulfate on the basis of an Eu-PB modified platinum electrode as the working electrode. The analyte was injected to separation capillary of 50 cm le...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Electrochemistry |
| Online Access: | http://dx.doi.org/10.4061/2011/403691 |
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| author | Min Zhou Juan Mi Yujie Li Huashan Zhang Yanjun Fang |
| author_facet | Min Zhou Juan Mi Yujie Li Huashan Zhang Yanjun Fang |
| author_sort | Min Zhou |
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| description | A Ru(bpy)3 2+-based electrochemiluminescence (ECL) detection coupled with capillary electrophoresis (CE) was developed for the determination of atropine sulfate on the basis of an Eu-PB modified platinum electrode as the working electrode. The analyte was injected to separation capillary of 50 cm length (25 μm i.d., 360 μm o.d.) by electrokinetic injection for 10 s at 10 kV. Parameters related to the separation and detection were discussed and optimized. It was proved that 10 mM phosphate buffer at pH 8.0 could achieve the most favorable resolution, and the high sensitivity of detection was obtained by using the detection potential at 1.15 V and 5 mM Ru(bpy)3 2+ in 80 mM phosphate buffer at pH 8.0 in the detection reservoir. Under the optimized conditions, the ECL peak area was in proportion to atropine sulfate concentration in the range from 0.08 to 20 μg⋅mL−1
with a detection limit of 50 ng⋅mL−1 (3σ). The relative standard derivations of migration time and peak area were 0.81 and 3.19%, respectively. The developed method was successfully applied to determine the levels of atropine sulfate in urine samples of patients with recoveries between 90.9 and 98.6%. |
| format | Article |
| id | doaj-art-cf943c0eea644c9eb1c074b6932698e5 |
| institution | Kabale University |
| issn | 2090-3537 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Electrochemistry |
| spelling | doaj-art-cf943c0eea644c9eb1c074b6932698e52025-02-03T00:59:12ZengWileyInternational Journal of Electrochemistry2090-35372011-01-01201110.4061/2011/403691403691Determination of Atropine Sulfate in Human Urines by Capillary Electrophoresis Using Chemical Modified Electrode as Electrochemiluminescence SensorMin Zhou0Juan Mi1Yujie Li2Huashan Zhang3Yanjun Fang4Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, and College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, ChinaKey Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, and College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, ChinaKey Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, and College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, ChinaInstitute of Hygienic and Environmental Medicinal Science, Key Laboratory of Risk Assessment and Control for Environment & Food Safety, Tianjin 300050, ChinaKey Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, and College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, ChinaA Ru(bpy)3 2+-based electrochemiluminescence (ECL) detection coupled with capillary electrophoresis (CE) was developed for the determination of atropine sulfate on the basis of an Eu-PB modified platinum electrode as the working electrode. The analyte was injected to separation capillary of 50 cm length (25 μm i.d., 360 μm o.d.) by electrokinetic injection for 10 s at 10 kV. Parameters related to the separation and detection were discussed and optimized. It was proved that 10 mM phosphate buffer at pH 8.0 could achieve the most favorable resolution, and the high sensitivity of detection was obtained by using the detection potential at 1.15 V and 5 mM Ru(bpy)3 2+ in 80 mM phosphate buffer at pH 8.0 in the detection reservoir. Under the optimized conditions, the ECL peak area was in proportion to atropine sulfate concentration in the range from 0.08 to 20 μg⋅mL−1 with a detection limit of 50 ng⋅mL−1 (3σ). The relative standard derivations of migration time and peak area were 0.81 and 3.19%, respectively. The developed method was successfully applied to determine the levels of atropine sulfate in urine samples of patients with recoveries between 90.9 and 98.6%.http://dx.doi.org/10.4061/2011/403691 |
| spellingShingle | Min Zhou Juan Mi Yujie Li Huashan Zhang Yanjun Fang Determination of Atropine Sulfate in Human Urines by Capillary Electrophoresis Using Chemical Modified Electrode as Electrochemiluminescence Sensor International Journal of Electrochemistry |
| title | Determination of Atropine Sulfate in Human Urines by Capillary Electrophoresis Using Chemical Modified Electrode as Electrochemiluminescence Sensor |
| title_full | Determination of Atropine Sulfate in Human Urines by Capillary Electrophoresis Using Chemical Modified Electrode as Electrochemiluminescence Sensor |
| title_fullStr | Determination of Atropine Sulfate in Human Urines by Capillary Electrophoresis Using Chemical Modified Electrode as Electrochemiluminescence Sensor |
| title_full_unstemmed | Determination of Atropine Sulfate in Human Urines by Capillary Electrophoresis Using Chemical Modified Electrode as Electrochemiluminescence Sensor |
| title_short | Determination of Atropine Sulfate in Human Urines by Capillary Electrophoresis Using Chemical Modified Electrode as Electrochemiluminescence Sensor |
| title_sort | determination of atropine sulfate in human urines by capillary electrophoresis using chemical modified electrode as electrochemiluminescence sensor |
| url | http://dx.doi.org/10.4061/2011/403691 |
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