Heat stress stimulates colon cancer cells to secret specific population of extracellular nanovesicles enriched by HSP70 and microRNAs
Background. Heat stress (HS) induces the cellular secretion of heat shock proteins (HSP ) and extracellular nanovesicles (ENVs). The biological link between these phenomena is poorly understood. In the case of colorectal cancer (CRC) cells, the secretion of HSP s and ENV may be involved in the clini...
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2022-03-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/2028 |
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| author | I. V. Nazarova L. M. Zabegina N. S. Nikiforova M. A. Slusarenko E. I. Sidina A. V. Zhakhov A. M. Ishchenko B. A. Margulis I. V. Guzhova A. V. Malek |
| author_facet | I. V. Nazarova L. M. Zabegina N. S. Nikiforova M. A. Slusarenko E. I. Sidina A. V. Zhakhov A. M. Ishchenko B. A. Margulis I. V. Guzhova A. V. Malek |
| author_sort | I. V. Nazarova |
| collection | DOAJ |
| description | Background. Heat stress (HS) induces the cellular secretion of heat shock proteins (HSP ) and extracellular nanovesicles (ENVs). The biological link between these phenomena is poorly understood. In the case of colorectal cancer (CRC) cells, the secretion of HSP s and ENV may be involved in the clinical response to intraperitoneal therapy of peritoneal carcinomatosis.Material and Methods. Established colon cancer cell lines COLO 320, HCT 116, HT29 and DLD 1 were used. ENVs were isolated from culture media by differential ultra-centrifugation and analyzed by dynamic light scattering, nanoparticle tracking analysis, atomic force microscopy and flow cytometry. Super-paramagnetic particles (SPMP ) covered by antibodies to the membrane form of Hsp70 were used for isolation and quantification of Hsp70(+) ENVs. Vesicular microRNA was assayed by RT-qPC R.Results. HS induces the secretion of ENVs by CRC cells, the resistance to HS correlates with the activity of HS-induced ENVs secretion. HS induces the secretion of a specific population of ENVs enriched by membrane form Hsp70 (mHsp70). The microRNA content of mHsp70(+) ENVs has qualitative and quantitative features. The concentration of miR-126-3p, -181-5p, -155-5p, -223 is increased in mHSP 70(+) ENVs secreted by three CRC cell lines.Conclusion. HS induces the secretion of mHSP 70(+) ENVs by CRC cells. This phenomenon may be involved in a clinical response to intraperitoneal chemo-hyperthermic perfusion therapy of peritoneal carcinomatosis. |
| format | Article |
| id | doaj-art-cf922b241cef45eda32ade98e3074d0b |
| institution | DOAJ |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2022-03-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-cf922b241cef45eda32ade98e3074d0b2025-08-20T02:59:10ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682022-03-01211577110.21294/1814-4861-2022-21-1-57-71961Heat stress stimulates colon cancer cells to secret specific population of extracellular nanovesicles enriched by HSP70 and microRNAsI. V. Nazarova0L. M. Zabegina1N. S. Nikiforova2M. A. Slusarenko3E. I. Sidina4A. V. Zhakhov5A. M. Ishchenko6B. A. Margulis7I. V. Guzhova8A. V. Malek9N.N. Petrov National Medical Research Center of OncologyN.N. Petrov National Medical Research Center of OncologyN.N. Petrov National Medical Research Center of OncologyN.N. Petrov National Medical Research Center of OncologyN.N. Petrov National Medical Research Center of OncologyInstitute of Highly Pure BiopreparationsInstitute of Highly Pure BiopreparationsInstitute of Cytology, RASInstitute of Cytology, RASN.N. Petrov National Medical Research Center of Oncology; Oncosystem Ltd.Background. Heat stress (HS) induces the cellular secretion of heat shock proteins (HSP ) and extracellular nanovesicles (ENVs). The biological link between these phenomena is poorly understood. In the case of colorectal cancer (CRC) cells, the secretion of HSP s and ENV may be involved in the clinical response to intraperitoneal therapy of peritoneal carcinomatosis.Material and Methods. Established colon cancer cell lines COLO 320, HCT 116, HT29 and DLD 1 were used. ENVs were isolated from culture media by differential ultra-centrifugation and analyzed by dynamic light scattering, nanoparticle tracking analysis, atomic force microscopy and flow cytometry. Super-paramagnetic particles (SPMP ) covered by antibodies to the membrane form of Hsp70 were used for isolation and quantification of Hsp70(+) ENVs. Vesicular microRNA was assayed by RT-qPC R.Results. HS induces the secretion of ENVs by CRC cells, the resistance to HS correlates with the activity of HS-induced ENVs secretion. HS induces the secretion of a specific population of ENVs enriched by membrane form Hsp70 (mHsp70). The microRNA content of mHsp70(+) ENVs has qualitative and quantitative features. The concentration of miR-126-3p, -181-5p, -155-5p, -223 is increased in mHSP 70(+) ENVs secreted by three CRC cell lines.Conclusion. HS induces the secretion of mHSP 70(+) ENVs by CRC cells. This phenomenon may be involved in a clinical response to intraperitoneal chemo-hyperthermic perfusion therapy of peritoneal carcinomatosis.https://www.siboncoj.ru/jour/article/view/2028extracellular nanovesiclescolorectal cancerheat stressmicrorna |
| spellingShingle | I. V. Nazarova L. M. Zabegina N. S. Nikiforova M. A. Slusarenko E. I. Sidina A. V. Zhakhov A. M. Ishchenko B. A. Margulis I. V. Guzhova A. V. Malek Heat stress stimulates colon cancer cells to secret specific population of extracellular nanovesicles enriched by HSP70 and microRNAs Сибирский онкологический журнал extracellular nanovesicles colorectal cancer heat stress microrna |
| title | Heat stress stimulates colon cancer cells to secret specific population of extracellular nanovesicles enriched by HSP70 and microRNAs |
| title_full | Heat stress stimulates colon cancer cells to secret specific population of extracellular nanovesicles enriched by HSP70 and microRNAs |
| title_fullStr | Heat stress stimulates colon cancer cells to secret specific population of extracellular nanovesicles enriched by HSP70 and microRNAs |
| title_full_unstemmed | Heat stress stimulates colon cancer cells to secret specific population of extracellular nanovesicles enriched by HSP70 and microRNAs |
| title_short | Heat stress stimulates colon cancer cells to secret specific population of extracellular nanovesicles enriched by HSP70 and microRNAs |
| title_sort | heat stress stimulates colon cancer cells to secret specific population of extracellular nanovesicles enriched by hsp70 and micrornas |
| topic | extracellular nanovesicles colorectal cancer heat stress microrna |
| url | https://www.siboncoj.ru/jour/article/view/2028 |
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