A multi-omics prognostic model of cuproptosis affects the prognosis of stomach adenocarcinoma

Abstract Background Cuproptosis, a form of cell death associated with copper ions, has been linked to the pathogenesis of various cancers, including gastric cancer. Investigating the role of cuproptosis-related genes through multi-omics analysis can enhance our understanding of disease mechanisms an...

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Main Authors: Yinying Wu, Yangwei Fan, Xuyuan Dong, Danfeng Dong, Yu Shi, Meichen Wang, Jia Wang, Yuqian Yang, Nan Yang, Fengyun Ou, Enxiao Li
Format: Article
Language:English
Published: BMC 2025-06-01
Series:Clinical Epigenetics
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Online Access:https://doi.org/10.1186/s13148-025-01894-0
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author Yinying Wu
Yangwei Fan
Xuyuan Dong
Danfeng Dong
Yu Shi
Meichen Wang
Jia Wang
Yuqian Yang
Nan Yang
Fengyun Ou
Enxiao Li
author_facet Yinying Wu
Yangwei Fan
Xuyuan Dong
Danfeng Dong
Yu Shi
Meichen Wang
Jia Wang
Yuqian Yang
Nan Yang
Fengyun Ou
Enxiao Li
author_sort Yinying Wu
collection DOAJ
description Abstract Background Cuproptosis, a form of cell death associated with copper ions, has been linked to the pathogenesis of various cancers, including gastric cancer. Investigating the role of cuproptosis-related genes through multi-omics analysis can enhance our understanding of disease mechanisms and improve prognosis prediction. Objective This study aims to elucidate the role of cuproptosis-related genes in gastric cancer from a multi-omics perspective. Materials and Methods We utilized multi-omics sequencing data from TCGA and GEO databases to explore the relationships between cuproptosis genes and gastric carcinogenesis, clinical phenotypes, and prognosis. This analysis encompassed mutation, copy number variation, methylation, mRNA expression, alternative splicing, and APA alterations. Additionally, we examined the regulatory roles of cuproptosis genes in gastric cancer through ceRNA interactions, gene mutations, and DNA methylation. A multi-omics prognostic model for gastric cancer was subsequently constructed. Results Our findings revealed that CDKN2A was the most frequently mutated gene in gastric cancer. Overall mutations in cuproptosis genes and copy number alterations of PDHB significantly impacted gastric cancer prognosis. Methylation, alternative splicing, and APA alterations of CDKN2A also influenced patient outcomes. Notably, MTF1, a key gene in cuproptosis, was found to affect apoptosis and invasion in gastric cancer cell lines. Conclusion We successfully developed a multi-omics prognostic model for gastric cancer that offers significant predictive value for patient outcomes.
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spelling doaj-art-cf8458220dd646d39f810c0ec0d2d0512025-08-20T03:21:03ZengBMCClinical Epigenetics1868-70832025-06-0117111610.1186/s13148-025-01894-0A multi-omics prognostic model of cuproptosis affects the prognosis of stomach adenocarcinomaYinying Wu0Yangwei Fan1Xuyuan Dong2Danfeng Dong3Yu Shi4Meichen Wang5Jia Wang6Yuqian Yang7Nan Yang8Fengyun Ou9Enxiao Li10Department of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Medical Oncology, the First Affiliated Hospital of Xi’an Jiaotong UniversityAbstract Background Cuproptosis, a form of cell death associated with copper ions, has been linked to the pathogenesis of various cancers, including gastric cancer. Investigating the role of cuproptosis-related genes through multi-omics analysis can enhance our understanding of disease mechanisms and improve prognosis prediction. Objective This study aims to elucidate the role of cuproptosis-related genes in gastric cancer from a multi-omics perspective. Materials and Methods We utilized multi-omics sequencing data from TCGA and GEO databases to explore the relationships between cuproptosis genes and gastric carcinogenesis, clinical phenotypes, and prognosis. This analysis encompassed mutation, copy number variation, methylation, mRNA expression, alternative splicing, and APA alterations. Additionally, we examined the regulatory roles of cuproptosis genes in gastric cancer through ceRNA interactions, gene mutations, and DNA methylation. A multi-omics prognostic model for gastric cancer was subsequently constructed. Results Our findings revealed that CDKN2A was the most frequently mutated gene in gastric cancer. Overall mutations in cuproptosis genes and copy number alterations of PDHB significantly impacted gastric cancer prognosis. Methylation, alternative splicing, and APA alterations of CDKN2A also influenced patient outcomes. Notably, MTF1, a key gene in cuproptosis, was found to affect apoptosis and invasion in gastric cancer cell lines. Conclusion We successfully developed a multi-omics prognostic model for gastric cancer that offers significant predictive value for patient outcomes.https://doi.org/10.1186/s13148-025-01894-0CuproptosisStomach adenocarcinomaMulti-omics prognostic modelStomach adenocarcinoma
spellingShingle Yinying Wu
Yangwei Fan
Xuyuan Dong
Danfeng Dong
Yu Shi
Meichen Wang
Jia Wang
Yuqian Yang
Nan Yang
Fengyun Ou
Enxiao Li
A multi-omics prognostic model of cuproptosis affects the prognosis of stomach adenocarcinoma
Clinical Epigenetics
Cuproptosis
Stomach adenocarcinoma
Multi-omics prognostic model
Stomach adenocarcinoma
title A multi-omics prognostic model of cuproptosis affects the prognosis of stomach adenocarcinoma
title_full A multi-omics prognostic model of cuproptosis affects the prognosis of stomach adenocarcinoma
title_fullStr A multi-omics prognostic model of cuproptosis affects the prognosis of stomach adenocarcinoma
title_full_unstemmed A multi-omics prognostic model of cuproptosis affects the prognosis of stomach adenocarcinoma
title_short A multi-omics prognostic model of cuproptosis affects the prognosis of stomach adenocarcinoma
title_sort multi omics prognostic model of cuproptosis affects the prognosis of stomach adenocarcinoma
topic Cuproptosis
Stomach adenocarcinoma
Multi-omics prognostic model
Stomach adenocarcinoma
url https://doi.org/10.1186/s13148-025-01894-0
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