Unique pharmacological properties of etrasimod among S1P receptor modulators

Etrasimod (ADP334) is an oral, once‐daily, selective sphingosine 1‐phosphate (S1P)1,4,5 receptor modulator for the treatment of moderately to severely active ulcerative colitis and in development for the treatment of immune‐mediated inflammatory diseases. Interaction between S1P and its five recepto...

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Main Authors: Ibragim Gaidarov, H. Kiyomi Komori, Dariusz T. Stepniak, Karin Bruinsma, Huong Dang, Xiaohua Chen, Todd Anthony, Joel Gatlin, Lisa Karimi‐Naser, Anh‐Tuan Ton, Tim Indersmitten, Paul E. Miller, Andre Ghetti, Najah Abi‐Gerges, David Unett, Hussien Al‐Shamma, Christopher J. Rabbat, Catherine Crosby, John W. Adams
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:FEBS Open Bio
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Online Access:https://doi.org/10.1002/2211-5463.13907
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author Ibragim Gaidarov
H. Kiyomi Komori
Dariusz T. Stepniak
Karin Bruinsma
Huong Dang
Xiaohua Chen
Todd Anthony
Joel Gatlin
Lisa Karimi‐Naser
Anh‐Tuan Ton
Tim Indersmitten
Paul E. Miller
Andre Ghetti
Najah Abi‐Gerges
David Unett
Hussien Al‐Shamma
Christopher J. Rabbat
Catherine Crosby
John W. Adams
author_facet Ibragim Gaidarov
H. Kiyomi Komori
Dariusz T. Stepniak
Karin Bruinsma
Huong Dang
Xiaohua Chen
Todd Anthony
Joel Gatlin
Lisa Karimi‐Naser
Anh‐Tuan Ton
Tim Indersmitten
Paul E. Miller
Andre Ghetti
Najah Abi‐Gerges
David Unett
Hussien Al‐Shamma
Christopher J. Rabbat
Catherine Crosby
John W. Adams
author_sort Ibragim Gaidarov
collection DOAJ
description Etrasimod (ADP334) is an oral, once‐daily, selective sphingosine 1‐phosphate (S1P)1,4,5 receptor modulator for the treatment of moderately to severely active ulcerative colitis and in development for the treatment of immune‐mediated inflammatory diseases. Interaction between S1P and its five receptor subtypes (S1P1–S1P5) plays a role in several physiologic systems, including the cardiovascular and immune systems. Since differences in S1PR binding and downstream intracellular signaling could contribute to distinct profiles of drug efficacy and safety, we directly compared the S1P1–5 selectivity profile of etrasimod to three marketed S1PR modulators: fingolimod, ozanimod, and siponimod. Using both heterologous expression systems and human umbilical vein endothelial cells that spontaneously express S1P1, we profiled key S1P1 downstream signaling pathways and found that etrasimod had similar potency to the other tested S1PR modulators in promoting β‐arrestin recruitment and S1P1 internalization. However, etrasimod was notably less potent than other S1PR modulators in assays measuring S1P1‐mediated G protein activation (GTPγS binding and cAMP inhibition). Relatively lower potency of etrasimod in inducing G protein signaling corresponded to significantly diminished activation of human cardiac G protein‐coupled inwardly rectifying potassium channels when compared to ozanimod. Together with pharmacokinetic properties, this pharmacologic profile of etrasimod may contribute to the positive benefit risk profile of etrasimod observed during the phase III ELEVATE UC 52 and ELEVATE UC 12 trials in patients with moderately to severely active ulcerative colitis.
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spelling doaj-art-cf79b53711654d2c96b13570cbd1bd082025-08-20T01:48:25ZengWileyFEBS Open Bio2211-54632025-01-0115110812110.1002/2211-5463.13907Unique pharmacological properties of etrasimod among S1P receptor modulatorsIbragim Gaidarov0H. Kiyomi Komori1Dariusz T. Stepniak2Karin Bruinsma3Huong Dang4Xiaohua Chen5Todd Anthony6Joel Gatlin7Lisa Karimi‐Naser8Anh‐Tuan Ton9Tim Indersmitten10Paul E. Miller11Andre Ghetti12Najah Abi‐Gerges13David Unett14Hussien Al‐Shamma15Christopher J. Rabbat16Catherine Crosby17John W. Adams18Beacon Discovery San Diego CA USAArena Pharmaceuticals San Diego CA USAArena Pharmaceuticals San Diego CA USABeacon Discovery San Diego CA USABeacon Discovery San Diego CA USABeacon Discovery San Diego CA USABeacon Discovery San Diego CA USABeacon Discovery San Diego CA USAArena Pharmaceuticals San Diego CA USAAnaBios Corporation San Diego CA USAAnaBios Corporation San Diego CA USAAnaBios Corporation San Diego CA USAAnaBios Corporation San Diego CA USAAnaBios Corporation San Diego CA USABeacon Discovery San Diego CA USABeacon Discovery San Diego CA USAArena Pharmaceuticals San Diego CA USAArena Pharmaceuticals San Diego CA USAArena Pharmaceuticals San Diego CA USAEtrasimod (ADP334) is an oral, once‐daily, selective sphingosine 1‐phosphate (S1P)1,4,5 receptor modulator for the treatment of moderately to severely active ulcerative colitis and in development for the treatment of immune‐mediated inflammatory diseases. Interaction between S1P and its five receptor subtypes (S1P1–S1P5) plays a role in several physiologic systems, including the cardiovascular and immune systems. Since differences in S1PR binding and downstream intracellular signaling could contribute to distinct profiles of drug efficacy and safety, we directly compared the S1P1–5 selectivity profile of etrasimod to three marketed S1PR modulators: fingolimod, ozanimod, and siponimod. Using both heterologous expression systems and human umbilical vein endothelial cells that spontaneously express S1P1, we profiled key S1P1 downstream signaling pathways and found that etrasimod had similar potency to the other tested S1PR modulators in promoting β‐arrestin recruitment and S1P1 internalization. However, etrasimod was notably less potent than other S1PR modulators in assays measuring S1P1‐mediated G protein activation (GTPγS binding and cAMP inhibition). Relatively lower potency of etrasimod in inducing G protein signaling corresponded to significantly diminished activation of human cardiac G protein‐coupled inwardly rectifying potassium channels when compared to ozanimod. Together with pharmacokinetic properties, this pharmacologic profile of etrasimod may contribute to the positive benefit risk profile of etrasimod observed during the phase III ELEVATE UC 52 and ELEVATE UC 12 trials in patients with moderately to severely active ulcerative colitis.https://doi.org/10.1002/2211-5463.13907etrasimodpharmacodynamicspharmacokineticssphingosine 1‐phosphate receptor modulator
spellingShingle Ibragim Gaidarov
H. Kiyomi Komori
Dariusz T. Stepniak
Karin Bruinsma
Huong Dang
Xiaohua Chen
Todd Anthony
Joel Gatlin
Lisa Karimi‐Naser
Anh‐Tuan Ton
Tim Indersmitten
Paul E. Miller
Andre Ghetti
Najah Abi‐Gerges
David Unett
Hussien Al‐Shamma
Christopher J. Rabbat
Catherine Crosby
John W. Adams
Unique pharmacological properties of etrasimod among S1P receptor modulators
FEBS Open Bio
etrasimod
pharmacodynamics
pharmacokinetics
sphingosine 1‐phosphate receptor modulator
title Unique pharmacological properties of etrasimod among S1P receptor modulators
title_full Unique pharmacological properties of etrasimod among S1P receptor modulators
title_fullStr Unique pharmacological properties of etrasimod among S1P receptor modulators
title_full_unstemmed Unique pharmacological properties of etrasimod among S1P receptor modulators
title_short Unique pharmacological properties of etrasimod among S1P receptor modulators
title_sort unique pharmacological properties of etrasimod among s1p receptor modulators
topic etrasimod
pharmacodynamics
pharmacokinetics
sphingosine 1‐phosphate receptor modulator
url https://doi.org/10.1002/2211-5463.13907
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