Formulation and phytochemical analysis of a potentially functional beverage from combining a leaf extract and pulp of Annona muricata (Annonaceae)

Background and Aims: Annona muricata beverages are widely used in traditional medicine to treat different ailments; however, they have been little characterized phytochemically and are consumed as sugary beverages, and may contribute to non-communicable diseases, including obesity. The objective of...

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Main Authors: Yolanda Nolasco-González, María de Lourdes García-Magaña, Andrés Eloy León-Fernández, Juan L. Monribot-Villanueva, José A. Guerrero-Analco, Efigenia Montalvo González
Format: Article
Language:English
Published: Instituto de Ecología A.C. 2023-07-01
Series:Acta Botanica Mexicana
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Online Access:https://abm.ojs.inecol.mx/index.php/abm/article/view/2185
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author Yolanda Nolasco-González
María de Lourdes García-Magaña
Andrés Eloy León-Fernández
Juan L. Monribot-Villanueva
José A. Guerrero-Analco
Efigenia Montalvo González
author_facet Yolanda Nolasco-González
María de Lourdes García-Magaña
Andrés Eloy León-Fernández
Juan L. Monribot-Villanueva
José A. Guerrero-Analco
Efigenia Montalvo González
author_sort Yolanda Nolasco-González
collection DOAJ
description Background and Aims: Annona muricata beverages are widely used in traditional medicine to treat different ailments; however, they have been little characterized phytochemically and are consumed as sugary beverages, and may contribute to non-communicable diseases, including obesity. The objective of this work was to obtain and characterize a sensorially acceptable beverage from an extract of A. muricata leaves and pulp. Methods: A mixture design was used to formulate nine beverages by combining different ratios of the aqueous extract of A. muricata leaves and pulp. These beverages were subjected to physicochemical and sensory evaluation. Based on these results, one beverage was selected to be characterized by spectrophotometry and compared with an infused and commercial beverage. In addition, the selected beverage was further analyzed for its phytochemical composition by ultra-high-performance chromatography coupled to mass spectrometry and high-performance liquid chromatography coupled to a photodiode array detector. Key results: The selected beverage was formulated with 25% pulp, 50% leaf extract, and 25% water; it also displayed a 7.63 °Brix, pH 3.42, and 0.40% acidity. This beverage was up to three times higher in bioactive compounds than infused and commercial beverages. Fourteen phenolic compounds and two acetogenins were quantified, while two alkaloids (coclaurine and reticuline) and fifteen acetogenins were tentatively identified. Conclusions: The combination of A. muricata leaf extract and pulp is a vital alternative to obtain a sensorially acceptable beverage that contains bioactive compounds with a potential medicinal action since the phenolic compounds, alkaloids, and acetogenins present in the beverage could have efficient biological activities in the prevention of chronic degenerative diseases.
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spelling doaj-art-cf74f2bf0c8e4382ab46bc4107f0e0a72025-08-20T02:56:39ZengInstituto de Ecología A.C.Acta Botanica Mexicana2448-75892023-07-0113010.21829/abm130.2023.2185Formulation and phytochemical analysis of a potentially functional beverage from combining a leaf extract and pulp of Annona muricata (Annonaceae)Yolanda Nolasco-González0María de Lourdes García-Magaña1Andrés Eloy León-Fernández2Juan L. Monribot-Villanueva3https://orcid.org/0000-0003-2394-0524José A. Guerrero-Analco4https://orcid.org/0000-0003-0998-757XEfigenia Montalvo González5https://orcid.org/0000-0002-3760-0806Instituto Tecnológico de Tepic e Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Campo Experimental Santiago IxcuintlaInstituto Tecnológico de TepicUniversidad Autónoma de NayaritInstituto de Ecología, A.C., Red de Estudios Moleculares AvanzadosInstituto de Ecología, A.C., Red de Estudios Moleculares AvanzadosInstituto Tecnológico de Tepic Background and Aims: Annona muricata beverages are widely used in traditional medicine to treat different ailments; however, they have been little characterized phytochemically and are consumed as sugary beverages, and may contribute to non-communicable diseases, including obesity. The objective of this work was to obtain and characterize a sensorially acceptable beverage from an extract of A. muricata leaves and pulp. Methods: A mixture design was used to formulate nine beverages by combining different ratios of the aqueous extract of A. muricata leaves and pulp. These beverages were subjected to physicochemical and sensory evaluation. Based on these results, one beverage was selected to be characterized by spectrophotometry and compared with an infused and commercial beverage. In addition, the selected beverage was further analyzed for its phytochemical composition by ultra-high-performance chromatography coupled to mass spectrometry and high-performance liquid chromatography coupled to a photodiode array detector. Key results: The selected beverage was formulated with 25% pulp, 50% leaf extract, and 25% water; it also displayed a 7.63 °Brix, pH 3.42, and 0.40% acidity. This beverage was up to three times higher in bioactive compounds than infused and commercial beverages. Fourteen phenolic compounds and two acetogenins were quantified, while two alkaloids (coclaurine and reticuline) and fifteen acetogenins were tentatively identified. Conclusions: The combination of A. muricata leaf extract and pulp is a vital alternative to obtain a sensorially acceptable beverage that contains bioactive compounds with a potential medicinal action since the phenolic compounds, alkaloids, and acetogenins present in the beverage could have efficient biological activities in the prevention of chronic degenerative diseases. https://abm.ojs.inecol.mx/index.php/abm/article/view/2185drinksmixture designphytochemicalssensory analysissoursop
spellingShingle Yolanda Nolasco-González
María de Lourdes García-Magaña
Andrés Eloy León-Fernández
Juan L. Monribot-Villanueva
José A. Guerrero-Analco
Efigenia Montalvo González
Formulation and phytochemical analysis of a potentially functional beverage from combining a leaf extract and pulp of Annona muricata (Annonaceae)
Acta Botanica Mexicana
drinks
mixture design
phytochemicals
sensory analysis
soursop
title Formulation and phytochemical analysis of a potentially functional beverage from combining a leaf extract and pulp of Annona muricata (Annonaceae)
title_full Formulation and phytochemical analysis of a potentially functional beverage from combining a leaf extract and pulp of Annona muricata (Annonaceae)
title_fullStr Formulation and phytochemical analysis of a potentially functional beverage from combining a leaf extract and pulp of Annona muricata (Annonaceae)
title_full_unstemmed Formulation and phytochemical analysis of a potentially functional beverage from combining a leaf extract and pulp of Annona muricata (Annonaceae)
title_short Formulation and phytochemical analysis of a potentially functional beverage from combining a leaf extract and pulp of Annona muricata (Annonaceae)
title_sort formulation and phytochemical analysis of a potentially functional beverage from combining a leaf extract and pulp of annona muricata annonaceae
topic drinks
mixture design
phytochemicals
sensory analysis
soursop
url https://abm.ojs.inecol.mx/index.php/abm/article/view/2185
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