Spectroscopic investigation of two xanthane dyes and design of a FRET based pesticide sensor
Abstract Layer-by-Layer (LbL) technique is the simplest and inexpensive method for preparartion of nano-dimensional thin films for tailoring material behavior having wide range of applications including sensors. Here, spectroscopic behavior of two laser dyes Acriflavine (Acf) and Rhodamine B (RhB) a...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84846-7 |
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| author | Sangita Majumder Subrata Deb Shazidul Hussain Dibyendu Dey Debajyoti Bhattacharjee Abdullah N. Alodhayb Shamima Hussain Syed Arshad Hussain |
| author_facet | Sangita Majumder Subrata Deb Shazidul Hussain Dibyendu Dey Debajyoti Bhattacharjee Abdullah N. Alodhayb Shamima Hussain Syed Arshad Hussain |
| author_sort | Sangita Majumder |
| collection | DOAJ |
| description | Abstract Layer-by-Layer (LbL) technique is the simplest and inexpensive method for preparartion of nano-dimensional thin films for tailoring material behavior having wide range of applications including sensors. Here, spectroscopic behavior of two laser dyes Acriflavine (Acf) and Rhodamine B (RhB) assembled onto LbL films have been investigated. It has been observed that both Acf and RhB form stable LbL films. Polyanion polyacrylic acid (PAA) was used to incorporate the Acf or RhB onto the LbL films. Adsorption of Acf and RhB onto PAA were completed within 45 min and 30 min respectively. During LbL film, material loss occurred in case of Acf. It has been demonstrated that such material loss can be minimized by incorporating clay laponite onto the LbL films. Temperature and pH dependant studies indicate that Acf and RhB assembled onto LbL films can be used to design temperature as well as pH sensors. Fluorescence Resonance Energy Transfer (FRET) between Acf and RhB has also been investigated. Interestingly, it has been demonstrated that the energy transfer efficiency can be manipulated using spacer molecules within the Acf and RhB LbL films. Laponite clay can be used to enhance the FRET efficiency, whereas stearic acid (SA) can be used to lower the efficiency. FRET efficiency linearly changes upon exposure of a pesticide pretilachlor at varying concentration. This study indicated that with proper calibration, proposed sensing system can be used to design FRET based pesticide sensor with detection limit of 0.22 ppm. |
| format | Article |
| id | doaj-art-cf65004402ff49a3b950db578d93a338 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-cf65004402ff49a3b950db578d93a3382025-01-26T12:26:03ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-024-84846-7Spectroscopic investigation of two xanthane dyes and design of a FRET based pesticide sensorSangita Majumder0Subrata Deb1Shazidul Hussain2Dibyendu Dey3Debajyoti Bhattacharjee4Abdullah N. Alodhayb5Shamima Hussain6Syed Arshad Hussain7Thin Films and Nanoscience Laboratory, Department of Physics, Tripura UniversityDepartment of Physics, Women’s CollegeThin Films and Nanoscience Laboratory, Department of Physics, Tripura UniversityDepartment of Basic science and Humanities, Techno College of EngineeringThin Films and Nanoscience Laboratory, Department of Physics, Tripura UniversityKing Abdullah Institute for Nanotechnology, King Saud UniversityUGC-DAE-CSR, Kalpakkam NodeThin Films and Nanoscience Laboratory, Department of Physics, Tripura UniversityAbstract Layer-by-Layer (LbL) technique is the simplest and inexpensive method for preparartion of nano-dimensional thin films for tailoring material behavior having wide range of applications including sensors. Here, spectroscopic behavior of two laser dyes Acriflavine (Acf) and Rhodamine B (RhB) assembled onto LbL films have been investigated. It has been observed that both Acf and RhB form stable LbL films. Polyanion polyacrylic acid (PAA) was used to incorporate the Acf or RhB onto the LbL films. Adsorption of Acf and RhB onto PAA were completed within 45 min and 30 min respectively. During LbL film, material loss occurred in case of Acf. It has been demonstrated that such material loss can be minimized by incorporating clay laponite onto the LbL films. Temperature and pH dependant studies indicate that Acf and RhB assembled onto LbL films can be used to design temperature as well as pH sensors. Fluorescence Resonance Energy Transfer (FRET) between Acf and RhB has also been investigated. Interestingly, it has been demonstrated that the energy transfer efficiency can be manipulated using spacer molecules within the Acf and RhB LbL films. Laponite clay can be used to enhance the FRET efficiency, whereas stearic acid (SA) can be used to lower the efficiency. FRET efficiency linearly changes upon exposure of a pesticide pretilachlor at varying concentration. This study indicated that with proper calibration, proposed sensing system can be used to design FRET based pesticide sensor with detection limit of 0.22 ppm.https://doi.org/10.1038/s41598-024-84846-7FRETDyesClayPretilachlorPAASA |
| spellingShingle | Sangita Majumder Subrata Deb Shazidul Hussain Dibyendu Dey Debajyoti Bhattacharjee Abdullah N. Alodhayb Shamima Hussain Syed Arshad Hussain Spectroscopic investigation of two xanthane dyes and design of a FRET based pesticide sensor Scientific Reports FRET Dyes Clay Pretilachlor PAA SA |
| title | Spectroscopic investigation of two xanthane dyes and design of a FRET based pesticide sensor |
| title_full | Spectroscopic investigation of two xanthane dyes and design of a FRET based pesticide sensor |
| title_fullStr | Spectroscopic investigation of two xanthane dyes and design of a FRET based pesticide sensor |
| title_full_unstemmed | Spectroscopic investigation of two xanthane dyes and design of a FRET based pesticide sensor |
| title_short | Spectroscopic investigation of two xanthane dyes and design of a FRET based pesticide sensor |
| title_sort | spectroscopic investigation of two xanthane dyes and design of a fret based pesticide sensor |
| topic | FRET Dyes Clay Pretilachlor PAA SA |
| url | https://doi.org/10.1038/s41598-024-84846-7 |
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