Isolation and determination of Acanthamoeba genotypes in surface water of Ilam city
Acanthamoeba, a widely distributed free-living amoeba with 20 genotypes identified through rRNA gene sequencing, exhibits varying degrees of pathogenicity influenced by its genotype. This study focuses on assessing the prevalence of Acanthamoeba species in the surface waters of Ilam, located in west...
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| Format: | Article |
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Ilam University of Medical Sciences
2024-09-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
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| Online Access: | http://sjimu.medilam.ac.ir/article-1-8417-en.pdf |
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| author | Azadeh Ghadesi Hamid Hasanpour Razi Naserifar Jahangir Abdi Mohammad Reza Mahmoudi Habibollah Turki Omid Raiesi Muhammad Ibrahim Getso Asad Mirzaei |
| author_facet | Azadeh Ghadesi Hamid Hasanpour Razi Naserifar Jahangir Abdi Mohammad Reza Mahmoudi Habibollah Turki Omid Raiesi Muhammad Ibrahim Getso Asad Mirzaei |
| author_sort | Azadeh Ghadesi |
| collection | DOAJ |
| description | Acanthamoeba, a widely distributed free-living amoeba with 20 genotypes identified through rRNA gene sequencing, exhibits varying degrees of pathogenicity influenced by its genotype. This study focuses on assessing the prevalence of Acanthamoeba species in the surface waters of Ilam, located in western Iran, utilizing morphological analysis and sequencing of the 18S rRNA gene through the PCR method. A total of 50 water samples were collected from various regions within Ilam city, situated in the southwest of Iran. To isolate Acanthamoeba parasites from the samples, a culture method was used, and all utilized culture media were scrutinized through microscopic and molecular techniques. The parasite’s genotype was determined by sequencing a 500-bp fragment of the 18S rRNA gene. Using microscopic and molecular methods, 19 and 16 water samples tested positive, respectively. The 18S rRNA sequences revealed that the isolates belonged to the T4, T2, and T11 genotypes. This study emphasizes the presence and inclination for close contact with highly pathogenic genotypes of Acanthamoeba in the surface waters of Ilam City. |
| format | Article |
| id | doaj-art-cf5f546c868a4de88f0c20e27dda53fe |
| institution | Kabale University |
| issn | 1563-4728 2588-3135 |
| language | fas |
| publishDate | 2024-09-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj-art-cf5f546c868a4de88f0c20e27dda53fe2025-08-25T07:34:32ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352024-09-01324133136Isolation and determination of Acanthamoeba genotypes in surface water of Ilam cityAzadeh Ghadesi0Hamid Hasanpour1Razi Naserifar2Jahangir Abdi3Mohammad Reza Mahmoudi4Habibollah Turki5Omid Raiesi6Muhammad Ibrahim Getso7Asad Mirzaei8 Dept of Parasitology and Mycology, School of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran Dept of Parasitology and Mycology, School of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran Dept of Parasitology and Mycology, School of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran Dept of Parasitology and Mycology, School of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran Dept of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Student Research Committee, Ilam University of Medical Sciences, Ilam, Iran Dept of Medical Microbiology and Parasitology, College of Health Sciences, Bayero University Kano, PMB 3011, Kano, Nigeria Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran Acanthamoeba, a widely distributed free-living amoeba with 20 genotypes identified through rRNA gene sequencing, exhibits varying degrees of pathogenicity influenced by its genotype. This study focuses on assessing the prevalence of Acanthamoeba species in the surface waters of Ilam, located in western Iran, utilizing morphological analysis and sequencing of the 18S rRNA gene through the PCR method. A total of 50 water samples were collected from various regions within Ilam city, situated in the southwest of Iran. To isolate Acanthamoeba parasites from the samples, a culture method was used, and all utilized culture media were scrutinized through microscopic and molecular techniques. The parasite’s genotype was determined by sequencing a 500-bp fragment of the 18S rRNA gene. Using microscopic and molecular methods, 19 and 16 water samples tested positive, respectively. The 18S rRNA sequences revealed that the isolates belonged to the T4, T2, and T11 genotypes. This study emphasizes the presence and inclination for close contact with highly pathogenic genotypes of Acanthamoeba in the surface waters of Ilam City.http://sjimu.medilam.ac.ir/article-1-8417-en.pdfacanthamoebagenotypesiranwater18srrna |
| spellingShingle | Azadeh Ghadesi Hamid Hasanpour Razi Naserifar Jahangir Abdi Mohammad Reza Mahmoudi Habibollah Turki Omid Raiesi Muhammad Ibrahim Getso Asad Mirzaei Isolation and determination of Acanthamoeba genotypes in surface water of Ilam city Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām acanthamoeba genotypes iran water 18srrna |
| title | Isolation and determination of Acanthamoeba genotypes in surface water of Ilam city |
| title_full | Isolation and determination of Acanthamoeba genotypes in surface water of Ilam city |
| title_fullStr | Isolation and determination of Acanthamoeba genotypes in surface water of Ilam city |
| title_full_unstemmed | Isolation and determination of Acanthamoeba genotypes in surface water of Ilam city |
| title_short | Isolation and determination of Acanthamoeba genotypes in surface water of Ilam city |
| title_sort | isolation and determination of acanthamoeba genotypes in surface water of ilam city |
| topic | acanthamoeba genotypes iran water 18srrna |
| url | http://sjimu.medilam.ac.ir/article-1-8417-en.pdf |
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