The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility
The sigma factor 54 (σ54) controls the expression of many genes in response to nutritional and environmental conditions. There are two σ54 genes, rpoN1 (XAC1969) and rpoN2 (XAC2972), in Xanthomonas citri subsp. citri. To investigate their functions, the deletion mutants ΔrpoN1, ΔrpoN2 and ΔrpoN1N2 w...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2016-09-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209531191561317X |
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| author | Gibson Kamau Gicharu Dong-ling SUN Xun HU Xiao-jing FAN Tao ZHUO Chuan-wan WU Hua-song ZOU |
| author_facet | Gibson Kamau Gicharu Dong-ling SUN Xun HU Xiao-jing FAN Tao ZHUO Chuan-wan WU Hua-song ZOU |
| author_sort | Gibson Kamau Gicharu |
| collection | DOAJ |
| description | The sigma factor 54 (σ54) controls the expression of many genes in response to nutritional and environmental conditions. There are two σ54 genes, rpoN1 (XAC1969) and rpoN2 (XAC2972), in Xanthomonas citri subsp. citri. To investigate their functions, the deletion mutants ΔrpoN1, ΔrpoN2 and ΔrpoN1N2 were constructed in this study. All the mutants delayed canker development in low concentration inoculation in citrus plants. The bacterial growth of mutants was retarded in the medium supplemented with nitrogen and carbon resources. Under either condition, the influence degree caused by deletion of rpoN2 was larger than the deletion of rpoN1. Remarkably, the mutant ΔrpoN1 showed a reduction in cell motility, while the mutant ΔrpoN2 increased cell motility. Our data suggested that the rpoN1 and rpoN2 play diverse roles in X. citri subsp. citri. |
| format | Article |
| id | doaj-art-cf5c8de618db462ab0b1fd280a9673b8 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-cf5c8de618db462ab0b1fd280a9673b82025-08-20T03:57:44ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-09-011592032203910.1016/S2095-3119(15)61317-XThe sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motilityGibson Kamau Gicharu0Dong-ling SUN1Xun HU2Xiao-jing FAN3Tao ZHUO4Chuan-wan WU5Hua-song ZOU6Fujian Province Key Laboratory of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaFujian Province Key Laboratory of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaFujian Province Key Laboratory of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaFujian Province Key Laboratory of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaFujian Province Key Laboratory of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaHuai'an Institute of Agricultural Sciences, Huai'an 223001, P.R.ChinaFujian Province Key Laboratory of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; Correspondence ZOU Hua-song, Tel: +86-591-8378469The sigma factor 54 (σ54) controls the expression of many genes in response to nutritional and environmental conditions. There are two σ54 genes, rpoN1 (XAC1969) and rpoN2 (XAC2972), in Xanthomonas citri subsp. citri. To investigate their functions, the deletion mutants ΔrpoN1, ΔrpoN2 and ΔrpoN1N2 were constructed in this study. All the mutants delayed canker development in low concentration inoculation in citrus plants. The bacterial growth of mutants was retarded in the medium supplemented with nitrogen and carbon resources. Under either condition, the influence degree caused by deletion of rpoN2 was larger than the deletion of rpoN1. Remarkably, the mutant ΔrpoN1 showed a reduction in cell motility, while the mutant ΔrpoN2 increased cell motility. Our data suggested that the rpoN1 and rpoN2 play diverse roles in X. citri subsp. citri.http://www.sciencedirect.com/science/article/pii/S209531191561317XXanthomonas citri subsp. citrirpoNcell motilitycitrus cankerfull virulence |
| spellingShingle | Gibson Kamau Gicharu Dong-ling SUN Xun HU Xiao-jing FAN Tao ZHUO Chuan-wan WU Hua-song ZOU The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility Journal of Integrative Agriculture Xanthomonas citri subsp. citri rpoN cell motility citrus canker full virulence |
| title | The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility |
| title_full | The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility |
| title_fullStr | The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility |
| title_full_unstemmed | The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility |
| title_short | The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility |
| title_sort | sigma 54 genes rpon1 and rpon2 of xanthomonas citri subsp citri play different roles in virulence nutrient utilization and cell motility |
| topic | Xanthomonas citri subsp. citri rpoN cell motility citrus canker full virulence |
| url | http://www.sciencedirect.com/science/article/pii/S209531191561317X |
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