Thiamine is a vitamin for plant-pathogenic powdery mildew fungi
Summary: Thiamine (vitamin B1) is an essential micronutrient in all forms of life that serves as a cofactor for several enzymes in primary metabolism. Plant-pathogenic and obligate biotrophic powdery mildew fungi appear to be auxotrophic for the micronutrient, as they have lost the majority of the g...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225013847 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849246033755766784 |
|---|---|
| author | Björn Sabelleck Matthias Freh Meltem Lammertz Jennifer Browatzki Anna-Maria Vllaho Mariola Piślewska-Bednarek Paweł Bednarek Ralph Panstruga |
| author_facet | Björn Sabelleck Matthias Freh Meltem Lammertz Jennifer Browatzki Anna-Maria Vllaho Mariola Piślewska-Bednarek Paweł Bednarek Ralph Panstruga |
| author_sort | Björn Sabelleck |
| collection | DOAJ |
| description | Summary: Thiamine (vitamin B1) is an essential micronutrient in all forms of life that serves as a cofactor for several enzymes in primary metabolism. Plant-pathogenic and obligate biotrophic powdery mildew fungi appear to be auxotrophic for the micronutrient, as they have lost the majority of the genes needed for thiamine biosynthesis. Using the barley powdery mildew pathogen, Blumeria hordei, as a study object, we found that (1) asexual conidiospores contain detectable levels of thiamine, (2) the B. hordei THI80 gene encodes a functional thiamine pyrophosphokinase, and (3) B. hordei has a functional DUR31-like thiamine transporter likely localizing to the cell surface. We further demonstrated a requirement of the metabolite for the activity of B. hordei transketolase, an enzyme of central carbon metabolism known to be thiamine-dependent in other organisms. Taken together, our data suggest that thiamine is a vitamin for B. hordei and, thus, likely all powdery mildew fungi. |
| format | Article |
| id | doaj-art-cf3d64eba4d74d4abc26774ff0ff5f58 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-cf3d64eba4d74d4abc26774ff0ff5f582025-08-20T03:58:36ZengElsevieriScience2589-00422025-08-0128811312310.1016/j.isci.2025.113123Thiamine is a vitamin for plant-pathogenic powdery mildew fungiBjörn Sabelleck0Matthias Freh1Meltem Lammertz2Jennifer Browatzki3Anna-Maria Vllaho4Mariola Piślewska-Bednarek5Paweł Bednarek6Ralph Panstruga7Unit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1 52056 Aachen, GermanyUnit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1 52056 Aachen, GermanyUnit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1 52056 Aachen, GermanyUnit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1 52056 Aachen, GermanyUnit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1 52056 Aachen, GermanyInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14 61-704 Poznań, PolandInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14 61-704 Poznań, PolandUnit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1 52056 Aachen, Germany; Corresponding authorSummary: Thiamine (vitamin B1) is an essential micronutrient in all forms of life that serves as a cofactor for several enzymes in primary metabolism. Plant-pathogenic and obligate biotrophic powdery mildew fungi appear to be auxotrophic for the micronutrient, as they have lost the majority of the genes needed for thiamine biosynthesis. Using the barley powdery mildew pathogen, Blumeria hordei, as a study object, we found that (1) asexual conidiospores contain detectable levels of thiamine, (2) the B. hordei THI80 gene encodes a functional thiamine pyrophosphokinase, and (3) B. hordei has a functional DUR31-like thiamine transporter likely localizing to the cell surface. We further demonstrated a requirement of the metabolite for the activity of B. hordei transketolase, an enzyme of central carbon metabolism known to be thiamine-dependent in other organisms. Taken together, our data suggest that thiamine is a vitamin for B. hordei and, thus, likely all powdery mildew fungi.http://www.sciencedirect.com/science/article/pii/S2589004225013847plant biochemistryinteraction of plants with organismsmolecular plant pathologyplant pathology |
| spellingShingle | Björn Sabelleck Matthias Freh Meltem Lammertz Jennifer Browatzki Anna-Maria Vllaho Mariola Piślewska-Bednarek Paweł Bednarek Ralph Panstruga Thiamine is a vitamin for plant-pathogenic powdery mildew fungi iScience plant biochemistry interaction of plants with organisms molecular plant pathology plant pathology |
| title | Thiamine is a vitamin for plant-pathogenic powdery mildew fungi |
| title_full | Thiamine is a vitamin for plant-pathogenic powdery mildew fungi |
| title_fullStr | Thiamine is a vitamin for plant-pathogenic powdery mildew fungi |
| title_full_unstemmed | Thiamine is a vitamin for plant-pathogenic powdery mildew fungi |
| title_short | Thiamine is a vitamin for plant-pathogenic powdery mildew fungi |
| title_sort | thiamine is a vitamin for plant pathogenic powdery mildew fungi |
| topic | plant biochemistry interaction of plants with organisms molecular plant pathology plant pathology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225013847 |
| work_keys_str_mv | AT bjornsabelleck thiamineisavitaminforplantpathogenicpowderymildewfungi AT matthiasfreh thiamineisavitaminforplantpathogenicpowderymildewfungi AT meltemlammertz thiamineisavitaminforplantpathogenicpowderymildewfungi AT jenniferbrowatzki thiamineisavitaminforplantpathogenicpowderymildewfungi AT annamariavllaho thiamineisavitaminforplantpathogenicpowderymildewfungi AT mariolapislewskabednarek thiamineisavitaminforplantpathogenicpowderymildewfungi AT pawełbednarek thiamineisavitaminforplantpathogenicpowderymildewfungi AT ralphpanstruga thiamineisavitaminforplantpathogenicpowderymildewfungi |