Synergistic Effects of <i>Paenibacillus polymyxa</i> NBmelon-1 Inoculation and Grafting Restructure of Rhizosphere Microbiome and Enhanced Disease Resistance in Melon Self-Rootstocks
Rhizosphere microorganisms play pivotal roles in mitigating the challenges associated with continuous cropping in melon cultivation. While grafting and plant growth-promoting rhizobacteria (PGPR) independently influence rhizosphere microbial communities, their combined effects remain largely unexplo...
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2025-05-01
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| author | Wenjie Dong Quanyu Zang Yuhong Wang Erlei Ma Weihong Ding Leiyan Yan Fangmin Hao |
| author_facet | Wenjie Dong Quanyu Zang Yuhong Wang Erlei Ma Weihong Ding Leiyan Yan Fangmin Hao |
| author_sort | Wenjie Dong |
| collection | DOAJ |
| description | Rhizosphere microorganisms play pivotal roles in mitigating the challenges associated with continuous cropping in melon cultivation. While grafting and plant growth-promoting rhizobacteria (PGPR) independently influence rhizosphere microbial communities, their combined effects remain largely unexplored. This study investigates the synergistic regulation of <i>Paenibacillus polymyxa</i> NBmelon-1 inoculation and grafting on rhizosphere microbiome assembly, plant performance, and disease resistance in melon self-rootstocks. Field experiments demonstrated that NBmelon-1 inoculation significantly enhanced rootstock stem diameter (95.3% increase in spring) and root development, achieving a graft survival rate exceeding 95%. The combined treatment (NB+GJ) increased scion fruit yield by 29.8% in autumn and 36.5% in spring, as well as the single-fruit weight by 22.5% in autumn and 37.3% in spring, while maintaining fruit morphology. Integrated 16S rRNA and ITS sequencing revealed that the NB+GJ treatment selectively enriched antagonistic bacterial phyla (e.g., Firmicutes and Actinobacteriota) and suppressed pathogenic fungi (e.g., <i>Fusarium</i> and Melanconiella). Seasonal shifts in microbial diversity and functional gene profiles underscored the dynamic interplay between treatments and environmental factors. These findings establish a novel strategy for optimizing melon self-rootstock grafting systems and sustainably managing soil-borne diseases. |
| format | Article |
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| issn | 2076-2607 |
| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-cf3aada8ea304e82b62bb2df752c34db2025-08-20T02:20:59ZengMDPI AGMicroorganisms2076-26072025-05-01136117210.3390/microorganisms13061172Synergistic Effects of <i>Paenibacillus polymyxa</i> NBmelon-1 Inoculation and Grafting Restructure of Rhizosphere Microbiome and Enhanced Disease Resistance in Melon Self-RootstocksWenjie Dong0Quanyu Zang1Yuhong Wang2Erlei Ma3Weihong Ding4Leiyan Yan5Fangmin Hao6Ningbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding, Ningbo Academy of Agricultural Sciences, Ningbo 315040, ChinaNingbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding, Ningbo Academy of Agricultural Sciences, Ningbo 315040, ChinaNingbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding, Ningbo Academy of Agricultural Sciences, Ningbo 315040, ChinaNingbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding, Ningbo Academy of Agricultural Sciences, Ningbo 315040, ChinaNingbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding, Ningbo Academy of Agricultural Sciences, Ningbo 315040, ChinaNingbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding, Ningbo Academy of Agricultural Sciences, Ningbo 315040, ChinaNingbo Key Laboratory of Characteristic Horticultural Crops in Quality Adjustment and Resistance Breeding, Ningbo Academy of Agricultural Sciences, Ningbo 315040, ChinaRhizosphere microorganisms play pivotal roles in mitigating the challenges associated with continuous cropping in melon cultivation. While grafting and plant growth-promoting rhizobacteria (PGPR) independently influence rhizosphere microbial communities, their combined effects remain largely unexplored. This study investigates the synergistic regulation of <i>Paenibacillus polymyxa</i> NBmelon-1 inoculation and grafting on rhizosphere microbiome assembly, plant performance, and disease resistance in melon self-rootstocks. Field experiments demonstrated that NBmelon-1 inoculation significantly enhanced rootstock stem diameter (95.3% increase in spring) and root development, achieving a graft survival rate exceeding 95%. The combined treatment (NB+GJ) increased scion fruit yield by 29.8% in autumn and 36.5% in spring, as well as the single-fruit weight by 22.5% in autumn and 37.3% in spring, while maintaining fruit morphology. Integrated 16S rRNA and ITS sequencing revealed that the NB+GJ treatment selectively enriched antagonistic bacterial phyla (e.g., Firmicutes and Actinobacteriota) and suppressed pathogenic fungi (e.g., <i>Fusarium</i> and Melanconiella). Seasonal shifts in microbial diversity and functional gene profiles underscored the dynamic interplay between treatments and environmental factors. These findings establish a novel strategy for optimizing melon self-rootstock grafting systems and sustainably managing soil-borne diseases.https://www.mdpi.com/2076-2607/13/6/1172graftingPGPRsynergistic effectsrhizosphere microbiomesoil-borne disease control |
| spellingShingle | Wenjie Dong Quanyu Zang Yuhong Wang Erlei Ma Weihong Ding Leiyan Yan Fangmin Hao Synergistic Effects of <i>Paenibacillus polymyxa</i> NBmelon-1 Inoculation and Grafting Restructure of Rhizosphere Microbiome and Enhanced Disease Resistance in Melon Self-Rootstocks Microorganisms grafting PGPR synergistic effects rhizosphere microbiome soil-borne disease control |
| title | Synergistic Effects of <i>Paenibacillus polymyxa</i> NBmelon-1 Inoculation and Grafting Restructure of Rhizosphere Microbiome and Enhanced Disease Resistance in Melon Self-Rootstocks |
| title_full | Synergistic Effects of <i>Paenibacillus polymyxa</i> NBmelon-1 Inoculation and Grafting Restructure of Rhizosphere Microbiome and Enhanced Disease Resistance in Melon Self-Rootstocks |
| title_fullStr | Synergistic Effects of <i>Paenibacillus polymyxa</i> NBmelon-1 Inoculation and Grafting Restructure of Rhizosphere Microbiome and Enhanced Disease Resistance in Melon Self-Rootstocks |
| title_full_unstemmed | Synergistic Effects of <i>Paenibacillus polymyxa</i> NBmelon-1 Inoculation and Grafting Restructure of Rhizosphere Microbiome and Enhanced Disease Resistance in Melon Self-Rootstocks |
| title_short | Synergistic Effects of <i>Paenibacillus polymyxa</i> NBmelon-1 Inoculation and Grafting Restructure of Rhizosphere Microbiome and Enhanced Disease Resistance in Melon Self-Rootstocks |
| title_sort | synergistic effects of i paenibacillus polymyxa i nbmelon 1 inoculation and grafting restructure of rhizosphere microbiome and enhanced disease resistance in melon self rootstocks |
| topic | grafting PGPR synergistic effects rhizosphere microbiome soil-borne disease control |
| url | https://www.mdpi.com/2076-2607/13/6/1172 |
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