Establishment and application of an SNP molecular identification system for grape cultivars
We aimed to develop a set of single nucleotide polymorphism (SNP) markers that can be used to distinguish the main cultivated grape (Vitis L.) cultivars in China and provide technical support for domestic grape cultivar protection, cultivar registration, and market rights protection. A total of 517...
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KeAi Communications Co., Ltd.
2022-04-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921636547 |
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| author | Fu-qiang WANG Xiu-cai FAN Ying ZHANG Lei SUN Chong-huai LIU Jian-fu JIANG |
| author_facet | Fu-qiang WANG Xiu-cai FAN Ying ZHANG Lei SUN Chong-huai LIU Jian-fu JIANG |
| author_sort | Fu-qiang WANG |
| collection | DOAJ |
| description | We aimed to develop a set of single nucleotide polymorphism (SNP) markers that can be used to distinguish the main cultivated grape (Vitis L.) cultivars in China and provide technical support for domestic grape cultivar protection, cultivar registration, and market rights protection. A total of 517 high-quality loci were screened from 4 241 729 SNPs obtained by sequencing 304 grape accessions using specific locus amplified fragment sequencing, of which 442 were successfully designed as Kompetitive Allele Specific PCR (KASP) markers. A set of 27 markers that completely distinguishes 304 sequenced grape accessions was determined by using the program, and 26 effective markers were screened based on 23 representative grape cultivars. Finally, a total of 46 out of 48 KASP markers, including 22 markers selected by the research group in the early stage, were re-screened based on 348 grape accessions. Population structure, principal component, and cluster analyses all showed that the 348 grape accessions were best divided into two populations. In addition, cluster analysis subdivided them into six subpopulations. According to genetic distance, V. labrusca, V. davidii, V. heyneana, and V. amurensis were far from V. vinifera, while V. vinifera×V. labrusca and V. amurensis×V. vinifera were somewhere in between these two groups. Furthermore, a core set of 25 KASP markers could distinguish 95.69% of the 348 grape accessions, and the other 21 markers were used as extended markers. Therefore, SNP molecular markers based on KASP typing technology provide a new way for mapping DNA fingerprints in grape cultivars. With high efficiency and accuracy and low cost, this technology is more competitive than other current identification methods. It also has excellent application prospects in the grape distinctness, uniformity, and stability (DUS) test, as well as in promoting market rights protection in the near future. |
| format | Article |
| id | doaj-art-cf3262413d014bb29ba5f1ed64a10044 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-cf3262413d014bb29ba5f1ed64a100442025-08-20T03:57:44ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192022-04-012141044105710.1016/S2095-3119(21)63654-7Establishment and application of an SNP molecular identification system for grape cultivarsFu-qiang WANG0Xiu-cai FAN1Ying ZHANG2Lei SUN3Chong-huai LIU4Jian-fu JIANG5Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R.ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R.ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R.ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R.ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R.ChinaCorrespondence JIANG Jian-fu; Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R.ChinaWe aimed to develop a set of single nucleotide polymorphism (SNP) markers that can be used to distinguish the main cultivated grape (Vitis L.) cultivars in China and provide technical support for domestic grape cultivar protection, cultivar registration, and market rights protection. A total of 517 high-quality loci were screened from 4 241 729 SNPs obtained by sequencing 304 grape accessions using specific locus amplified fragment sequencing, of which 442 were successfully designed as Kompetitive Allele Specific PCR (KASP) markers. A set of 27 markers that completely distinguishes 304 sequenced grape accessions was determined by using the program, and 26 effective markers were screened based on 23 representative grape cultivars. Finally, a total of 46 out of 48 KASP markers, including 22 markers selected by the research group in the early stage, were re-screened based on 348 grape accessions. Population structure, principal component, and cluster analyses all showed that the 348 grape accessions were best divided into two populations. In addition, cluster analysis subdivided them into six subpopulations. According to genetic distance, V. labrusca, V. davidii, V. heyneana, and V. amurensis were far from V. vinifera, while V. vinifera×V. labrusca and V. amurensis×V. vinifera were somewhere in between these two groups. Furthermore, a core set of 25 KASP markers could distinguish 95.69% of the 348 grape accessions, and the other 21 markers were used as extended markers. Therefore, SNP molecular markers based on KASP typing technology provide a new way for mapping DNA fingerprints in grape cultivars. With high efficiency and accuracy and low cost, this technology is more competitive than other current identification methods. It also has excellent application prospects in the grape distinctness, uniformity, and stability (DUS) test, as well as in promoting market rights protection in the near future.http://www.sciencedirect.com/science/article/pii/S2095311921636547grapeKASP markervariety identificationfingerprintgenetic diversity analysis |
| spellingShingle | Fu-qiang WANG Xiu-cai FAN Ying ZHANG Lei SUN Chong-huai LIU Jian-fu JIANG Establishment and application of an SNP molecular identification system for grape cultivars Journal of Integrative Agriculture grape KASP marker variety identification fingerprint genetic diversity analysis |
| title | Establishment and application of an SNP molecular identification system for grape cultivars |
| title_full | Establishment and application of an SNP molecular identification system for grape cultivars |
| title_fullStr | Establishment and application of an SNP molecular identification system for grape cultivars |
| title_full_unstemmed | Establishment and application of an SNP molecular identification system for grape cultivars |
| title_short | Establishment and application of an SNP molecular identification system for grape cultivars |
| title_sort | establishment and application of an snp molecular identification system for grape cultivars |
| topic | grape KASP marker variety identification fingerprint genetic diversity analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921636547 |
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