The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis
Background/Aim. The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2022-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2022/0042-84502100072M.pdf |
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| author | Milutinović-Smiljanić Sanja Antonijević Đorđe Mićić Milutin Biočanin Vladimir Sjerobabin Nikola Petrović Božana Danilović Vesna Jokanović Vukoman |
| author_facet | Milutinović-Smiljanić Sanja Antonijević Đorđe Mićić Milutin Biočanin Vladimir Sjerobabin Nikola Petrović Božana Danilović Vesna Jokanović Vukoman |
| author_sort | Milutinović-Smiljanić Sanja |
| collection | DOAJ |
| description | Background/Aim. The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the influence of poly-lactide-co-glycolide (PLGA) and polyethyleneimine (PEI) as coatings for hydroxyapatite (HAP) bone carriers on bone tissue regenerative potential in rabbits’ calvarial defect. Methods. Calvarial defects measuring 6 mm in diameter were made in 19 skeletally mature rabbits. Defects were filled with one of the following materials: PLGA coated HAP (HAP + PLGA), PEI coated HAP (HAP + PEI), and bovine HAP – Bio-Oss® (positive control). Unfilled defects represented negative control. Histological analysis was performed in order to determine the inflammatory response of the host tissue. The formation of the new bone was evaluated using histomorphometric analysis. All analyses have been conducted in samples obtained 3, 6, and 9 weeks after implantation. Results. Three weeks post-implantation, a trend toward increased healing in the HAP + PLGA group compared to other investigated materials was noticed, with no statistically significant difference between the study groups (p > 0.05). However, after 6 and 9 weeks, significant healing was observed in favor of the HAP coated with PLGA compared to other groups (p < 0.05). Within this group, greater bone healing was observed compared to the HAP + PEI and Bio-Oss® groups. Conclusion. PLGA demonstrated greater coating potential compared to PEI with respect to osteogenesis improvement in bone reconstructive surgery. |
| format | Article |
| id | doaj-art-cf2dbbf40b0c416090bd8f3650f13f70 |
| institution | OA Journals |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-cf2dbbf40b0c416090bd8f3650f13f702025-08-20T02:04:28ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202022-01-0179101025103410.2298/VSP210513072M0042-84502100072MThe influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysisMilutinović-Smiljanić Sanja0https://orcid.org/0000-0003-1357-4970Antonijević Đorđe1Mićić Milutin2Biočanin Vladimir3https://orcid.org/0000-0002-1607-4069Sjerobabin Nikola4Petrović Božana5Danilović Vesna6Jokanović Vukoman7https://orcid.org/0000-0002-2976-8238University of Belgrade, Faculty of Dental Medicine, Department of General and Oral Histology and Embryology, Belgrade, SerbiaUniversity of Belgrade, Institute for Nuclear Science “Vinča”, Laboratory for Atomic Physics, Belgrade, Serbia + University of Belgrade, Faculty of Medicine, Laboratory for Anthropology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Laboratory for Anthropology, Belgrade, SerbiaUniversity of Business Academy, Faculty of Dentistry, Pančevo, SerbiaUniversity Clinical Hospital Center ‟Dr. Dragiša Mišović”, Belgrade, SerbiaUniversity of Belgrade, Institute for Nuclear Science “Vinča”, Laboratory for Atomic Physics, Belgrade, SerbiaUniversity of Belgrade, Faculty of Dental Medicine, Department of General and Oral Histology and Embryology, Belgrade, SerbiaUniversity of Belgrade, Institute for Nuclear Science “Vinča”, Laboratory for Atomic Physics, Belgrade, SerbiaBackground/Aim. The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the influence of poly-lactide-co-glycolide (PLGA) and polyethyleneimine (PEI) as coatings for hydroxyapatite (HAP) bone carriers on bone tissue regenerative potential in rabbits’ calvarial defect. Methods. Calvarial defects measuring 6 mm in diameter were made in 19 skeletally mature rabbits. Defects were filled with one of the following materials: PLGA coated HAP (HAP + PLGA), PEI coated HAP (HAP + PEI), and bovine HAP – Bio-Oss® (positive control). Unfilled defects represented negative control. Histological analysis was performed in order to determine the inflammatory response of the host tissue. The formation of the new bone was evaluated using histomorphometric analysis. All analyses have been conducted in samples obtained 3, 6, and 9 weeks after implantation. Results. Three weeks post-implantation, a trend toward increased healing in the HAP + PLGA group compared to other investigated materials was noticed, with no statistically significant difference between the study groups (p > 0.05). However, after 6 and 9 weeks, significant healing was observed in favor of the HAP coated with PLGA compared to other groups (p < 0.05). Within this group, greater bone healing was observed compared to the HAP + PEI and Bio-Oss® groups. Conclusion. PLGA demonstrated greater coating potential compared to PEI with respect to osteogenesis improvement in bone reconstructive surgery.http://www.doiserbia.nb.rs/img/doi/0042-8450/2022/0042-84502100072M.pdfbone regenerationhistological techniquesmaterials testingpolymersrabbits |
| spellingShingle | Milutinović-Smiljanić Sanja Antonijević Đorđe Mićić Milutin Biočanin Vladimir Sjerobabin Nikola Petrović Božana Danilović Vesna Jokanović Vukoman The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis Vojnosanitetski Pregled bone regeneration histological techniques materials testing polymers rabbits |
| title | The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis |
| title_full | The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis |
| title_fullStr | The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis |
| title_full_unstemmed | The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis |
| title_short | The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis |
| title_sort | influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects histomorphometric and histological analysis |
| topic | bone regeneration histological techniques materials testing polymers rabbits |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2022/0042-84502100072M.pdf |
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