Application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma
Objective To investigate the application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma. Methods From January 2016 to December 2023, 11 patients with a history of lung adenocarcinoma and cerebrospinal fluid metastasis were collected...
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Editorial Office of Journal of Guangxi Medical University
2024-08-01
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| Series: | Guangxi Yike Daxue xuebao |
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| Online Access: | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.08.010 |
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| author | WANG Yifan ZHANG Lanlan YANG Qiao FU Xin ZHANG Yilei GUO Ying CHEN Jia |
| author_facet | WANG Yifan ZHANG Lanlan YANG Qiao FU Xin ZHANG Yilei GUO Ying CHEN Jia |
| author_sort | WANG Yifan |
| collection | DOAJ |
| description | Objective To investigate the application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma. Methods From January 2016 to December 2023, 11 patients with a history of lung adenocarcinoma and cerebrospinal fluid metastasis were collected for cerebrospinal fluid cytology. The control group included 11 cases of metastatic lung adenocarcinoma in cerebrospinal fluid stained with hematoxylin and eosin or Papanicolaou staining, and the experimental group included the re- prepared or faded samples of the corresponding control group for immunocytochemical staining. The clinical data, cytological morphology, diagnostic methods and immunocytochemical staining results of the 11 cases were analyzed. Results In the control group, several atypical cells were observed in clusters or single cells scattered, with moderate to abundant cytoplasm, large and dark nuclei, and some cells had obvious nucleoli. In the experimental group, the metastatic lung adenocarcinoma was confirmed by TTF-1 and NapsinA immunocytochemical staining. Conclusion The prognosis of patients with leptomeningeal metastasis from lung adenocarcinoma is extremely poor. The definite diagnosis of cerebrospinal fluid cytology is of great significance for clinical practice. When the amount of cerebrospinal fluid specimens is limited and there are only a small number of atypical cells, immunocytochemical staining after re-preparing or fading can make a definite diagnosis to assist clinical treatment. |
| format | Article |
| id | doaj-art-cf2c412d9e6549619bfb36aabd069af3 |
| institution | DOAJ |
| issn | 1005-930X |
| language | zho |
| publishDate | 2024-08-01 |
| publisher | Editorial Office of Journal of Guangxi Medical University |
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| series | Guangxi Yike Daxue xuebao |
| spelling | doaj-art-cf2c412d9e6549619bfb36aabd069af32025-08-20T02:57:28ZzhoEditorial Office of Journal of Guangxi Medical UniversityGuangxi Yike Daxue xuebao1005-930X2024-08-014181171117510.16190/j.cnki.45-1211/r.2024.08.010gxykdxxb-41-8-1171Application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinomaWANG Yifan0ZHANG Lanlan1YANG Qiao2FU Xin3ZHANG Yilei4GUO Ying5CHEN Jia6Department of Pathology, Xi'an Daxing Hospital, Xi'an 710016, ChinaDepartment of Pathology, Xi'an Daxing Hospital, Xi'an 710016, ChinaDepartment of Pathology, Xi'an Daxing Hospital, Xi'an 710016, ChinaDepartment of Pathology, Xijing Hospital, the First Affiliated Hospital of Air Force Medical University, Xi'an 710032, ChinaSchool of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an 710065, ChinaDepartment of Pathology, Xi'an Daxing Hospital, Xi'an 710016, ChinaDepartment of Pathology, Xi'an Daxing Hospital, Xi'an 710016, ChinaObjective To investigate the application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma. Methods From January 2016 to December 2023, 11 patients with a history of lung adenocarcinoma and cerebrospinal fluid metastasis were collected for cerebrospinal fluid cytology. The control group included 11 cases of metastatic lung adenocarcinoma in cerebrospinal fluid stained with hematoxylin and eosin or Papanicolaou staining, and the experimental group included the re- prepared or faded samples of the corresponding control group for immunocytochemical staining. The clinical data, cytological morphology, diagnostic methods and immunocytochemical staining results of the 11 cases were analyzed. Results In the control group, several atypical cells were observed in clusters or single cells scattered, with moderate to abundant cytoplasm, large and dark nuclei, and some cells had obvious nucleoli. In the experimental group, the metastatic lung adenocarcinoma was confirmed by TTF-1 and NapsinA immunocytochemical staining. Conclusion The prognosis of patients with leptomeningeal metastasis from lung adenocarcinoma is extremely poor. The definite diagnosis of cerebrospinal fluid cytology is of great significance for clinical practice. When the amount of cerebrospinal fluid specimens is limited and there are only a small number of atypical cells, immunocytochemical staining after re-preparing or fading can make a definite diagnosis to assist clinical treatment.https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.08.010cerebrospinal fluidmetastatic lung adenocarcinomacolour fadingimmunocytochemical staining |
| spellingShingle | WANG Yifan ZHANG Lanlan YANG Qiao FU Xin ZHANG Yilei GUO Ying CHEN Jia Application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma Guangxi Yike Daxue xuebao cerebrospinal fluid metastatic lung adenocarcinoma colour fading immunocytochemical staining |
| title | Application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma |
| title_full | Application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma |
| title_fullStr | Application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma |
| title_full_unstemmed | Application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma |
| title_short | Application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma |
| title_sort | application of two immunocytochemical staining methods in the diagnosis of cerebrospinal fluid metastatic lung adenocarcinoma |
| topic | cerebrospinal fluid metastatic lung adenocarcinoma colour fading immunocytochemical staining |
| url | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.08.010 |
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