Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals
IntroductionGastropod hemocyanins are potent immunostimulants in mammals, a trait associated with their large molecular size and unusual glycosylation patterns. While the hemocyanin from the marine snail keyhole limpet (KLH), has been widely studied and successfully employed as a carrier/adjuvant in...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1490260/full |
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| author | Ignacio Rafael Chiumiento Ignacio Rafael Chiumiento María Alejandra Tricerri María Alejandra Tricerri María Fernanda Cortéz Santiago Ituarte Santiago Ituarte Julia Tau Karina Valeria Mariño Paola Lorena Smaldini Horacio Heras Horacio Heras Marcos Sebastián Dreon Marcos Sebastián Dreon |
| author_facet | Ignacio Rafael Chiumiento Ignacio Rafael Chiumiento María Alejandra Tricerri María Alejandra Tricerri María Fernanda Cortéz Santiago Ituarte Santiago Ituarte Julia Tau Karina Valeria Mariño Paola Lorena Smaldini Horacio Heras Horacio Heras Marcos Sebastián Dreon Marcos Sebastián Dreon |
| author_sort | Ignacio Rafael Chiumiento |
| collection | DOAJ |
| description | IntroductionGastropod hemocyanins are potent immunostimulants in mammals, a trait associated with their large molecular size and unusual glycosylation patterns. While the hemocyanin from the marine snail keyhole limpet (KLH), has been widely studied and successfully employed as a carrier/adjuvant in several immunological applications, as well as a non-specific immunostimulant for bladder cancer treatment, few other gastropod hemocyanins have been biochemically and immunologically characterized. In this work, we investigated the immunogenic properties of the hemocyanin from Pomacea canaliculata (PcH), an invasive south American freshwater snail. This species, known for its high reproductive rate and easy rearing, represents a promising source of potential biomedical compounds, including hemocyanin.MethodsEmploying flow cytometry, fluorescence microscopy, immunoassays, and quantitative PCR, we analysed the effects of PcH on THP-1 monocytes and their derived macrophages, as well as its ability to induce humoral response on C57BL/6 mice. Additionally, we evaluated the structural stability of PcH across a wide range of temperature and pH values.Results and discussionOur findings demonstrate that PcH is a structurally stable protein that not only triggers a pro-inflammatory effect on THP-1 derived-macrophages by increasing IL1-β and TNF-α levels, but also promotes phenotypic changes associated with the monocyte-to-macrophage differentiation. Moreover, the humoral response induced by PcH in mice was indistinguishable from that of KLH, highlighting the promising immunostimulatory properties of this freshwater snail hemocyanin. |
| format | Article |
| id | doaj-art-cf2194c844594d9396dea48235f8db1d |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-cf2194c844594d9396dea48235f8db1d2025-01-08T06:11:49ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011510.3389/fimmu.2024.14902601490260Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammalsIgnacio Rafael Chiumiento0Ignacio Rafael Chiumiento1María Alejandra Tricerri2María Alejandra Tricerri3María Fernanda Cortéz4Santiago Ituarte5Santiago Ituarte6Julia Tau7Karina Valeria Mariño8Paola Lorena Smaldini9Horacio Heras10Horacio Heras11Marcos Sebastián Dreon12Marcos Sebastián Dreon13Instituto de Investigaciones Bioquímicas de La Plata “Prof. Dr. Rodolfo R. Brenner”, (INIBIOLP), Universidad Nacional de La Plata (UNLP) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata, ArgentinaCátedra de Química Biológica, Facultad de Ciencias Naturales y Museo, UNLP, La Plata, ArgentinaInstituto de Investigaciones Bioquímicas de La Plata “Prof. Dr. Rodolfo R. Brenner”, (INIBIOLP), Universidad Nacional de La Plata (UNLP) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata, ArgentinaCátedra de Bioquímica Clínica I, Facultad de Ciencias Médicas, UNLP, La Plata, ArgentinaInstituto de Investigaciones Bioquímicas de La Plata “Prof. Dr. Rodolfo R. Brenner”, (INIBIOLP), Universidad Nacional de La Plata (UNLP) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata, ArgentinaInstituto de Investigaciones Bioquímicas de La Plata “Prof. Dr. Rodolfo R. Brenner”, (INIBIOLP), Universidad Nacional de La Plata (UNLP) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata, ArgentinaCátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, UNLP, La Plata, ArgentinaInstituto de Investigaciones Bioquímicas de La Plata “Prof. Dr. Rodolfo R. Brenner”, (INIBIOLP), Universidad Nacional de La Plata (UNLP) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata, ArgentinaLaboratorio de Glicómica Funcional y Molecular, Instituto de Biología y Medicina Experimental (IBYME), CONICET, Buenos Aires, ArgentinaInstituto de Estudios Inmunológicos y Fisiopatológicos (IIFP), UNLP - CONICET, La Plata, ArgentinaInstituto de Investigaciones Bioquímicas de La Plata “Prof. Dr. Rodolfo R. Brenner”, (INIBIOLP), Universidad Nacional de La Plata (UNLP) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata, ArgentinaCátedra de Química Biológica, Facultad de Ciencias Naturales y Museo, UNLP, La Plata, ArgentinaInstituto de Investigaciones Bioquímicas de La Plata “Prof. Dr. Rodolfo R. Brenner”, (INIBIOLP), Universidad Nacional de La Plata (UNLP) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata, ArgentinaCátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, UNLP, La Plata, ArgentinaIntroductionGastropod hemocyanins are potent immunostimulants in mammals, a trait associated with their large molecular size and unusual glycosylation patterns. While the hemocyanin from the marine snail keyhole limpet (KLH), has been widely studied and successfully employed as a carrier/adjuvant in several immunological applications, as well as a non-specific immunostimulant for bladder cancer treatment, few other gastropod hemocyanins have been biochemically and immunologically characterized. In this work, we investigated the immunogenic properties of the hemocyanin from Pomacea canaliculata (PcH), an invasive south American freshwater snail. This species, known for its high reproductive rate and easy rearing, represents a promising source of potential biomedical compounds, including hemocyanin.MethodsEmploying flow cytometry, fluorescence microscopy, immunoassays, and quantitative PCR, we analysed the effects of PcH on THP-1 monocytes and their derived macrophages, as well as its ability to induce humoral response on C57BL/6 mice. Additionally, we evaluated the structural stability of PcH across a wide range of temperature and pH values.Results and discussionOur findings demonstrate that PcH is a structurally stable protein that not only triggers a pro-inflammatory effect on THP-1 derived-macrophages by increasing IL1-β and TNF-α levels, but also promotes phenotypic changes associated with the monocyte-to-macrophage differentiation. Moreover, the humoral response induced by PcH in mice was indistinguishable from that of KLH, highlighting the promising immunostimulatory properties of this freshwater snail hemocyanin.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1490260/fullgastropodsmolluscrespiratory pigmentinnate immunitymacrophage |
| spellingShingle | Ignacio Rafael Chiumiento Ignacio Rafael Chiumiento María Alejandra Tricerri María Alejandra Tricerri María Fernanda Cortéz Santiago Ituarte Santiago Ituarte Julia Tau Karina Valeria Mariño Paola Lorena Smaldini Horacio Heras Horacio Heras Marcos Sebastián Dreon Marcos Sebastián Dreon Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals Frontiers in Immunology gastropods mollusc respiratory pigment innate immunity macrophage |
| title | Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals |
| title_full | Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals |
| title_fullStr | Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals |
| title_full_unstemmed | Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals |
| title_short | Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals |
| title_sort | pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals |
| topic | gastropods mollusc respiratory pigment innate immunity macrophage |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1490260/full |
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