Construction of a Microfluidic Platform for Quantitative Detection of Multiple Fusarium Toxins Based on Upconversion Encoded Microspheres
To achieve simultaneous and rapid detection of multiple mycotoxins, a microfluidic chip based on upconversion fluorescent-coded microspheres was constructed and integrated with in vitro diagnostic equipment to develop a quantitative detection platform for mycotoxin. The platform employed multi-color...
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The editorial department of Science and Technology of Food Industry
2025-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023030205 |
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| author | Shengjie WANG Yongli YE Jingdong SHAO Xiulan SUN Jiadi SUN |
| author_facet | Shengjie WANG Yongli YE Jingdong SHAO Xiulan SUN Jiadi SUN |
| author_sort | Shengjie WANG |
| collection | DOAJ |
| description | To achieve simultaneous and rapid detection of multiple mycotoxins, a microfluidic chip based on upconversion fluorescent-coded microspheres was constructed and integrated with in vitro diagnostic equipment to develop a quantitative detection platform for mycotoxin. The platform employed multi-color upconversion fluorescent-coded microspheres as detection probes and utilized microfluidic chips as detection carriers. It collected images under a dual-wavelength microscope system of the multi-color upconversion fluorescent-coded microspheres and derived the types and contents of toxins through algorithmic analysis of the obtained optical signals. The application effect of the established platform was verified by using corn flour as the food matrix and analyzing deoxynivalenol (DON), T-2 toxin, and fumonisins B1 (FB1). The average diameter of the prepared three-color upconversion nanoparticles was 28.81 nm, the average diameter after wrapping the inert shell was 41.26 nm, and the average diameter of the upconversion coded microspheres obtained after swelling was 10 μm. The optimized ratios of the three kinds of probe antigen antibody secondary antibodies are as follows: DON: 1:50, 1:100, and 1:50, T-2: 1:200, 1:400, and 1:200, FB1: 1:50, 1:100, and 1:50. The detection limits of DON, T-2, and FB1 were 41.35, 0.5044, and 35.45 ng/mL, respectively, with a standard addition recovery from 90.76% to 114.82%. In conclusion, the established mycotoxin detection platform provides an effective way to avoid mutual interference in multiple detections, and it can rapidly and accurately obtain quantitative analysis results. |
| format | Article |
| id | doaj-art-cf21842962ec4c34873b18b0b9b13489 |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-cf21842962ec4c34873b18b0b9b134892025-08-20T03:46:53ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-06-01461225926610.13386/j.issn1002-0306.20230302052023030205-12Construction of a Microfluidic Platform for Quantitative Detection of Multiple Fusarium Toxins Based on Upconversion Encoded MicrospheresShengjie WANG0Yongli YE1Jingdong SHAO2Xiulan SUN3Jiadi SUN4School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaComprehensive Technology Center of Zhangjiagang Customs, Suzhou 215600, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaTo achieve simultaneous and rapid detection of multiple mycotoxins, a microfluidic chip based on upconversion fluorescent-coded microspheres was constructed and integrated with in vitro diagnostic equipment to develop a quantitative detection platform for mycotoxin. The platform employed multi-color upconversion fluorescent-coded microspheres as detection probes and utilized microfluidic chips as detection carriers. It collected images under a dual-wavelength microscope system of the multi-color upconversion fluorescent-coded microspheres and derived the types and contents of toxins through algorithmic analysis of the obtained optical signals. The application effect of the established platform was verified by using corn flour as the food matrix and analyzing deoxynivalenol (DON), T-2 toxin, and fumonisins B1 (FB1). The average diameter of the prepared three-color upconversion nanoparticles was 28.81 nm, the average diameter after wrapping the inert shell was 41.26 nm, and the average diameter of the upconversion coded microspheres obtained after swelling was 10 μm. The optimized ratios of the three kinds of probe antigen antibody secondary antibodies are as follows: DON: 1:50, 1:100, and 1:50, T-2: 1:200, 1:400, and 1:200, FB1: 1:50, 1:100, and 1:50. The detection limits of DON, T-2, and FB1 were 41.35, 0.5044, and 35.45 ng/mL, respectively, with a standard addition recovery from 90.76% to 114.82%. In conclusion, the established mycotoxin detection platform provides an effective way to avoid mutual interference in multiple detections, and it can rapidly and accurately obtain quantitative analysis results.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023030205microfluidic chipupconversion encoded microspheremulti-channelmycotoxinquantitative detection |
| spellingShingle | Shengjie WANG Yongli YE Jingdong SHAO Xiulan SUN Jiadi SUN Construction of a Microfluidic Platform for Quantitative Detection of Multiple Fusarium Toxins Based on Upconversion Encoded Microspheres Shipin gongye ke-ji microfluidic chip upconversion encoded microsphere multi-channel mycotoxin quantitative detection |
| title | Construction of a Microfluidic Platform for Quantitative Detection of Multiple Fusarium Toxins Based on Upconversion Encoded Microspheres |
| title_full | Construction of a Microfluidic Platform for Quantitative Detection of Multiple Fusarium Toxins Based on Upconversion Encoded Microspheres |
| title_fullStr | Construction of a Microfluidic Platform for Quantitative Detection of Multiple Fusarium Toxins Based on Upconversion Encoded Microspheres |
| title_full_unstemmed | Construction of a Microfluidic Platform for Quantitative Detection of Multiple Fusarium Toxins Based on Upconversion Encoded Microspheres |
| title_short | Construction of a Microfluidic Platform for Quantitative Detection of Multiple Fusarium Toxins Based on Upconversion Encoded Microspheres |
| title_sort | construction of a microfluidic platform for quantitative detection of multiple fusarium toxins based on upconversion encoded microspheres |
| topic | microfluidic chip upconversion encoded microsphere multi-channel mycotoxin quantitative detection |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023030205 |
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