Pesticide susceptibility monitoring of fall armyworms (Spodoptera frugiperda (J.E. Smith)): a simple methodology for information-sharing among Southeast Asian countries
Abstract Background The fall armyworm, Spodoptera frugiperda, is a destructive moth pest. It is highly migratory and was first detected in Southeast Asia in 2018, rapidly becoming a major pest of corn production in this region. Monitoring the susceptibility of S. frugiperda populations is important...
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| Format: | Article |
| Language: | English |
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CABI
2023-06-01
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| Series: | CABI Agriculture and Bioscience |
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| Online Access: | https://doi.org/10.1186/s43170-023-00160-8 |
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| author | Supangkana Thirawut Woravit Sutjaritthammajariyangkun Artit Rukkasikorn Pruetthichat Punyawattoe Uraporn Noonart Youichi Kobori |
| author_facet | Supangkana Thirawut Woravit Sutjaritthammajariyangkun Artit Rukkasikorn Pruetthichat Punyawattoe Uraporn Noonart Youichi Kobori |
| author_sort | Supangkana Thirawut |
| collection | DOAJ |
| description | Abstract Background The fall armyworm, Spodoptera frugiperda, is a destructive moth pest. It is highly migratory and was first detected in Southeast Asia in 2018, rapidly becoming a major pest of corn production in this region. Monitoring the susceptibility of S. frugiperda populations is important for efficient insecticide resistance management. Because of the high mobility of this pest, information-sharing of susceptibility levels among neighboring countries is required for insecticide resistance management. To this end, we developed simple standard methods for pesticide susceptibility monitoring of S. frugiperda to contribute to information-sharing among Southeast Asian countries. Methods The developed methods included mass rearing of larvae using an artificial diet and bioassay by diet overlay. The lethal concentrations for 50% and 95% mortality (LC50 and LC95) and resistance coefficient values were calculated. We tested the susceptibilities of samples of S. frugiperda collected from the six major corn planting areas in Thailand to emamectin benzoate, spinetoram, chlorantraniliprole, indoxacarb, chlorfenapyr, and lufenuron using the developed methods. Results The mortality of artificial diet-fed larvae was higher than those fed corn leaves, especially in the early instars. However, more than half of the specimens reared on the artificial diet became pupae. In the case of three of the six pesticides, emamectin benzoate, indoxacarb, and chlorfenapyr, the LC50 values of the samples collected in 2021 and 2022 were significantly higher than those collected in 2019, indicating increasing resistance to those three pesticides. According to the resistance coefficient values, only samples from one area exhibited low resistance to lufenuron. Conclusions We developed a simple standardized methodology for Southeast Asian countries to compare insecticide susceptibility. The calculated LC50 and resistance coefficient values can be used as a baseline for monitoring the development of pesticide resistance in the region. The LC50 values of several pesticides have increased significantly over the years. However, the resistance coefficient values indicated that S. frugiperda developed low resistance to only one pesticide (lufenuron). This study offers helpful information for insecticide selection and improved resistance management of fall armyworms in Thailand. |
| format | Article |
| id | doaj-art-cf1ade63cad9417eb191caf5188bffd9 |
| institution | Kabale University |
| issn | 2662-4044 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | CABI |
| record_format | Article |
| series | CABI Agriculture and Bioscience |
| spelling | doaj-art-cf1ade63cad9417eb191caf5188bffd92025-02-03T06:52:47ZengCABICABI Agriculture and Bioscience2662-40442023-06-01411810.1186/s43170-023-00160-8Pesticide susceptibility monitoring of fall armyworms (Spodoptera frugiperda (J.E. Smith)): a simple methodology for information-sharing among Southeast Asian countriesSupangkana Thirawut0Woravit Sutjaritthammajariyangkun1Artit Rukkasikorn2Pruetthichat Punyawattoe3Uraporn Noonart4Youichi Kobori5Entomology and Zoology Group, Plant Protection Research and Development Office, Department of AgricultureEntomology and Zoology Group, Plant Protection Research and Development Office, Department of AgricultureEntomology and Zoology Group, Plant Protection Research and Development Office, Department of AgricultureEntomology and Zoology Group, Plant Protection Research and Development Office, Department of AgricultureEntomology and Zoology Group, Plant Protection Research and Development Office, Department of AgricultureJapan International Research Center for Agricultural SciencesAbstract Background The fall armyworm, Spodoptera frugiperda, is a destructive moth pest. It is highly migratory and was first detected in Southeast Asia in 2018, rapidly becoming a major pest of corn production in this region. Monitoring the susceptibility of S. frugiperda populations is important for efficient insecticide resistance management. Because of the high mobility of this pest, information-sharing of susceptibility levels among neighboring countries is required for insecticide resistance management. To this end, we developed simple standard methods for pesticide susceptibility monitoring of S. frugiperda to contribute to information-sharing among Southeast Asian countries. Methods The developed methods included mass rearing of larvae using an artificial diet and bioassay by diet overlay. The lethal concentrations for 50% and 95% mortality (LC50 and LC95) and resistance coefficient values were calculated. We tested the susceptibilities of samples of S. frugiperda collected from the six major corn planting areas in Thailand to emamectin benzoate, spinetoram, chlorantraniliprole, indoxacarb, chlorfenapyr, and lufenuron using the developed methods. Results The mortality of artificial diet-fed larvae was higher than those fed corn leaves, especially in the early instars. However, more than half of the specimens reared on the artificial diet became pupae. In the case of three of the six pesticides, emamectin benzoate, indoxacarb, and chlorfenapyr, the LC50 values of the samples collected in 2021 and 2022 were significantly higher than those collected in 2019, indicating increasing resistance to those three pesticides. According to the resistance coefficient values, only samples from one area exhibited low resistance to lufenuron. Conclusions We developed a simple standardized methodology for Southeast Asian countries to compare insecticide susceptibility. The calculated LC50 and resistance coefficient values can be used as a baseline for monitoring the development of pesticide resistance in the region. The LC50 values of several pesticides have increased significantly over the years. However, the resistance coefficient values indicated that S. frugiperda developed low resistance to only one pesticide (lufenuron). This study offers helpful information for insecticide selection and improved resistance management of fall armyworms in Thailand.https://doi.org/10.1186/s43170-023-00160-8CornIntegrated pest managementPesticide resistanceResistance managementThailand |
| spellingShingle | Supangkana Thirawut Woravit Sutjaritthammajariyangkun Artit Rukkasikorn Pruetthichat Punyawattoe Uraporn Noonart Youichi Kobori Pesticide susceptibility monitoring of fall armyworms (Spodoptera frugiperda (J.E. Smith)): a simple methodology for information-sharing among Southeast Asian countries CABI Agriculture and Bioscience Corn Integrated pest management Pesticide resistance Resistance management Thailand |
| title | Pesticide susceptibility monitoring of fall armyworms (Spodoptera frugiperda (J.E. Smith)): a simple methodology for information-sharing among Southeast Asian countries |
| title_full | Pesticide susceptibility monitoring of fall armyworms (Spodoptera frugiperda (J.E. Smith)): a simple methodology for information-sharing among Southeast Asian countries |
| title_fullStr | Pesticide susceptibility monitoring of fall armyworms (Spodoptera frugiperda (J.E. Smith)): a simple methodology for information-sharing among Southeast Asian countries |
| title_full_unstemmed | Pesticide susceptibility monitoring of fall armyworms (Spodoptera frugiperda (J.E. Smith)): a simple methodology for information-sharing among Southeast Asian countries |
| title_short | Pesticide susceptibility monitoring of fall armyworms (Spodoptera frugiperda (J.E. Smith)): a simple methodology for information-sharing among Southeast Asian countries |
| title_sort | pesticide susceptibility monitoring of fall armyworms spodoptera frugiperda j e smith a simple methodology for information sharing among southeast asian countries |
| topic | Corn Integrated pest management Pesticide resistance Resistance management Thailand |
| url | https://doi.org/10.1186/s43170-023-00160-8 |
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