Determination of Matrine in Rat Plasma after Oral Administration of Novel Korean Herbal Medicine KIOM-MA128 and Application of PK
KIOM-MA128 is a novel Korean herbal medicine with antiatopic, anti-inflammatory, and antiasthmatic effects. Matrine is thought to be a potential chemical marker of KIOM-MA128, but pharmacokinetic studies on KIOM-MA128 had not been performed. This study describes a simple and rapid method using high-...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/431632 |
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| author | Hyun-moon Back Byungjeong Song Jung-woo Chae Hwi-yeol Yun Jin Yeul Ma Kwang-il Kwon |
| author_facet | Hyun-moon Back Byungjeong Song Jung-woo Chae Hwi-yeol Yun Jin Yeul Ma Kwang-il Kwon |
| author_sort | Hyun-moon Back |
| collection | DOAJ |
| description | KIOM-MA128 is a novel Korean herbal medicine with antiatopic, anti-inflammatory, and antiasthmatic effects. Matrine is thought to be a potential chemical marker of KIOM-MA128, but pharmacokinetic studies on KIOM-MA128 had not been performed. This study describes a simple and rapid method using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to determine the concentration of matrine in rats plasma after administration of KIOM-MA128. The isocratic mobile phase consisted of methanol and distilled water, and the flow rate was 0.15 mL/min. The accuracy and precision of the assay, as well as stability tests, were performed in accordance with FDA regulations for the validation of bioanalytical methods. The half-life and Tmax of matrine after administration of KIOM-MA128 were 4.29 ± 2.20 h and 1.8 ± 1.23 h, respectively. Cmax and AUCinf of matrine after administration of KIOM-MA128 at 4 g/kg and 8 g/kg were 595.10 ± 182.91 ng/mL, 5336.77 ± 1503.84 ng/mL·h and 850.46 ± 120 ng/mL, 9583.10 ± 888.92 ng/mL·h, respectively. The validated method was successfully applied to a pharmacokinetic study in rats after oral administration of KIOM-MA128. |
| format | Article |
| id | doaj-art-cf18ec0ad3a141c79fbd67e2bade9485 |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-cf18ec0ad3a141c79fbd67e2bade94852025-08-20T02:19:30ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732015-01-01201510.1155/2015/431632431632Determination of Matrine in Rat Plasma after Oral Administration of Novel Korean Herbal Medicine KIOM-MA128 and Application of PKHyun-moon Back0Byungjeong Song1Jung-woo Chae2Hwi-yeol Yun3Jin Yeul Ma4Kwang-il Kwon5College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of KoreaCollege of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of KoreaCollege of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of KoreaCollege of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of KoreaHerbal Medicine Improvement Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of KoreaCollege of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of KoreaKIOM-MA128 is a novel Korean herbal medicine with antiatopic, anti-inflammatory, and antiasthmatic effects. Matrine is thought to be a potential chemical marker of KIOM-MA128, but pharmacokinetic studies on KIOM-MA128 had not been performed. This study describes a simple and rapid method using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to determine the concentration of matrine in rats plasma after administration of KIOM-MA128. The isocratic mobile phase consisted of methanol and distilled water, and the flow rate was 0.15 mL/min. The accuracy and precision of the assay, as well as stability tests, were performed in accordance with FDA regulations for the validation of bioanalytical methods. The half-life and Tmax of matrine after administration of KIOM-MA128 were 4.29 ± 2.20 h and 1.8 ± 1.23 h, respectively. Cmax and AUCinf of matrine after administration of KIOM-MA128 at 4 g/kg and 8 g/kg were 595.10 ± 182.91 ng/mL, 5336.77 ± 1503.84 ng/mL·h and 850.46 ± 120 ng/mL, 9583.10 ± 888.92 ng/mL·h, respectively. The validated method was successfully applied to a pharmacokinetic study in rats after oral administration of KIOM-MA128.http://dx.doi.org/10.1155/2015/431632 |
| spellingShingle | Hyun-moon Back Byungjeong Song Jung-woo Chae Hwi-yeol Yun Jin Yeul Ma Kwang-il Kwon Determination of Matrine in Rat Plasma after Oral Administration of Novel Korean Herbal Medicine KIOM-MA128 and Application of PK Journal of Analytical Methods in Chemistry |
| title | Determination of Matrine in Rat Plasma after Oral Administration of Novel Korean Herbal Medicine KIOM-MA128 and Application of PK |
| title_full | Determination of Matrine in Rat Plasma after Oral Administration of Novel Korean Herbal Medicine KIOM-MA128 and Application of PK |
| title_fullStr | Determination of Matrine in Rat Plasma after Oral Administration of Novel Korean Herbal Medicine KIOM-MA128 and Application of PK |
| title_full_unstemmed | Determination of Matrine in Rat Plasma after Oral Administration of Novel Korean Herbal Medicine KIOM-MA128 and Application of PK |
| title_short | Determination of Matrine in Rat Plasma after Oral Administration of Novel Korean Herbal Medicine KIOM-MA128 and Application of PK |
| title_sort | determination of matrine in rat plasma after oral administration of novel korean herbal medicine kiom ma128 and application of pk |
| url | http://dx.doi.org/10.1155/2015/431632 |
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