NK cell-like behavior of Valpha14i NK T cells during MCMV infection.
Immunity to the murine cytomegalovirus (MCMV) is critically dependent on the innate response for initial containment of viral replication, resolution of active infection, and proper induction of the adaptive phase of the anti-viral response. In contrast to NK cells, the Valpha14 invariant natural ki...
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| Language: | English |
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Public Library of Science (PLoS)
2008-07-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1000106&type=printable |
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| author | Johnna D Wesley Marlowe S Tessmer Deanna Chaukos Laurent Brossay |
| author_facet | Johnna D Wesley Marlowe S Tessmer Deanna Chaukos Laurent Brossay |
| author_sort | Johnna D Wesley |
| collection | DOAJ |
| description | Immunity to the murine cytomegalovirus (MCMV) is critically dependent on the innate response for initial containment of viral replication, resolution of active infection, and proper induction of the adaptive phase of the anti-viral response. In contrast to NK cells, the Valpha14 invariant natural killer T cell response to MCMV has not been examined. We found that Valpha14i NK T cells become activated and produce significant levels of IFN-gamma, but do not proliferate or produce IL-4 following MCMV infection. In vivo treatment with an anti-CD1d mAb and adoptive transfer of Valpha14i NK T cells into MCMV-infected CD1d(-/-) mice demonstrate that CD1d is dispensable for Valpha14i NK T cell activation. In contrast, both IFN-alpha/beta and IL-12 are required for optimal activation. Valpha14i NK T cell-derived IFN-gamma is partially dependent on IFN-alpha/beta but highly dependent on IL-12. Valpha14i NK T cells contribute to the immune response to MCMV and amplify NK cell-derived IFN-gamma. Importantly, mortality is increased in CD1d(-/-) mice in response to high dose MCMV infection when compared to heterozygote littermate controls. Collectively, these findings illustrate the plasticity of Valpha14i NK T cells that act as effector T cells during bacterial infection, but have NK cell-like behavior during the innate immune response to MCMV infection. |
| format | Article |
| id | doaj-art-cf0c8f4b9ceb4e539891e22eb9329a32 |
| institution | OA Journals |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2008-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-cf0c8f4b9ceb4e539891e22eb9329a322025-08-20T02:17:29ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742008-07-0147e100010610.1371/journal.ppat.1000106NK cell-like behavior of Valpha14i NK T cells during MCMV infection.Johnna D WesleyMarlowe S TessmerDeanna ChaukosLaurent BrossayImmunity to the murine cytomegalovirus (MCMV) is critically dependent on the innate response for initial containment of viral replication, resolution of active infection, and proper induction of the adaptive phase of the anti-viral response. In contrast to NK cells, the Valpha14 invariant natural killer T cell response to MCMV has not been examined. We found that Valpha14i NK T cells become activated and produce significant levels of IFN-gamma, but do not proliferate or produce IL-4 following MCMV infection. In vivo treatment with an anti-CD1d mAb and adoptive transfer of Valpha14i NK T cells into MCMV-infected CD1d(-/-) mice demonstrate that CD1d is dispensable for Valpha14i NK T cell activation. In contrast, both IFN-alpha/beta and IL-12 are required for optimal activation. Valpha14i NK T cell-derived IFN-gamma is partially dependent on IFN-alpha/beta but highly dependent on IL-12. Valpha14i NK T cells contribute to the immune response to MCMV and amplify NK cell-derived IFN-gamma. Importantly, mortality is increased in CD1d(-/-) mice in response to high dose MCMV infection when compared to heterozygote littermate controls. Collectively, these findings illustrate the plasticity of Valpha14i NK T cells that act as effector T cells during bacterial infection, but have NK cell-like behavior during the innate immune response to MCMV infection.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1000106&type=printable |
| spellingShingle | Johnna D Wesley Marlowe S Tessmer Deanna Chaukos Laurent Brossay NK cell-like behavior of Valpha14i NK T cells during MCMV infection. PLoS Pathogens |
| title | NK cell-like behavior of Valpha14i NK T cells during MCMV infection. |
| title_full | NK cell-like behavior of Valpha14i NK T cells during MCMV infection. |
| title_fullStr | NK cell-like behavior of Valpha14i NK T cells during MCMV infection. |
| title_full_unstemmed | NK cell-like behavior of Valpha14i NK T cells during MCMV infection. |
| title_short | NK cell-like behavior of Valpha14i NK T cells during MCMV infection. |
| title_sort | nk cell like behavior of valpha14i nk t cells during mcmv infection |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1000106&type=printable |
| work_keys_str_mv | AT johnnadwesley nkcelllikebehaviorofvalpha14inktcellsduringmcmvinfection AT marlowestessmer nkcelllikebehaviorofvalpha14inktcellsduringmcmvinfection AT deannachaukos nkcelllikebehaviorofvalpha14inktcellsduringmcmvinfection AT laurentbrossay nkcelllikebehaviorofvalpha14inktcellsduringmcmvinfection |