Inflammatory responses and histopathological changes in a mouse model of Staphylococcus aureus-induced bloodstream infections
Introduction: Staphylococcus aureus-induced bloodstream infections (BSIs) remain a prevalent clinical challenge and the underlying pathogenesis is still poorly understood. The aim of this study was to investigate the inflammatory responses and histopathological changes in BSIs in mice. Methodology:...
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The Journal of Infection in Developing Countries
2017-04-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/7800 |
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| author | Dan Wu Shusheng Zhou Shijing Hu Bao Liu |
| author_facet | Dan Wu Shusheng Zhou Shijing Hu Bao Liu |
| author_sort | Dan Wu |
| collection | DOAJ |
| description | Introduction: Staphylococcus aureus-induced bloodstream infections (BSIs) remain a prevalent clinical challenge and the underlying pathogenesis is still poorly understood. The aim of this study was to investigate the inflammatory responses and histopathological changes in BSIs in mice.
Methodology: Male C57BL/6 mice were inoculated with S. aureus intravenously to induce BSIs. The survival rate, weight loss, and murine sepsis scores (MSS) were monitored in BSI and phosphate-buffered saline (PBS) control mice. Blood samples and tissue homogenates were plated on agar plates to determine the bacterial burden. Inflammatory proteins and cytokines were determined by enzyme-linked immunosorbent assay (ELISA) kits. Histopathologic changes were assessed by pathological inflammation score (PIS) and macroscopic and microscopic examinations.
Results: BSI mice induced by 4.5 × 108 CFU/mL S. aureus showed ~70% survival rate, higher sepsis scores, significantly decreased body weight, elevated levels of white blood cell (WBC) counts, C-reactive protein (CRP), procalcitonin (PCT), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. Prominent correlations were found between elevated CRP and PCT levels as well as among IL-1β, IL-6, and TNF-α. Pathological changes and higher PIS were also observed in BSI mice.
Conclusions: Our results demonstrate that inflammatory proteins (PCT and CRP) and cytokines (IL-6, IL-1β and TNF-α) play an important role in the inflammatory responses and histopathological changes in S. aureus-induced BSIs.
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| format | Article |
| id | doaj-art-cf0605bc70064e2d8fbf83e55ca8001b |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-cf0605bc70064e2d8fbf83e55ca8001b2025-08-20T02:27:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-04-01110410.3855/jidc.7800Inflammatory responses and histopathological changes in a mouse model of Staphylococcus aureus-induced bloodstream infectionsDan Wu0Shusheng Zhou1Shijing Hu2Bao Liu3Nanjing First Hospital, Nanjing Medical University, Nanjing, ChinaAffiliated Provincial Hospital of Anhui Medical University, Hefei, ChinaAffiliated Provincial Hospital of Anhui Medical University, Hefei, ChinaAffiliated Provincial Hospital of Anhui Medical University, Hefei, ChinaIntroduction: Staphylococcus aureus-induced bloodstream infections (BSIs) remain a prevalent clinical challenge and the underlying pathogenesis is still poorly understood. The aim of this study was to investigate the inflammatory responses and histopathological changes in BSIs in mice. Methodology: Male C57BL/6 mice were inoculated with S. aureus intravenously to induce BSIs. The survival rate, weight loss, and murine sepsis scores (MSS) were monitored in BSI and phosphate-buffered saline (PBS) control mice. Blood samples and tissue homogenates were plated on agar plates to determine the bacterial burden. Inflammatory proteins and cytokines were determined by enzyme-linked immunosorbent assay (ELISA) kits. Histopathologic changes were assessed by pathological inflammation score (PIS) and macroscopic and microscopic examinations. Results: BSI mice induced by 4.5 × 108 CFU/mL S. aureus showed ~70% survival rate, higher sepsis scores, significantly decreased body weight, elevated levels of white blood cell (WBC) counts, C-reactive protein (CRP), procalcitonin (PCT), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. Prominent correlations were found between elevated CRP and PCT levels as well as among IL-1β, IL-6, and TNF-α. Pathological changes and higher PIS were also observed in BSI mice. Conclusions: Our results demonstrate that inflammatory proteins (PCT and CRP) and cytokines (IL-6, IL-1β and TNF-α) play an important role in the inflammatory responses and histopathological changes in S. aureus-induced BSIs. https://jidc.org/index.php/journal/article/view/7800Staphylococcus aureusbloodstream infectionsinflammationcytokinesHistopathology |
| spellingShingle | Dan Wu Shusheng Zhou Shijing Hu Bao Liu Inflammatory responses and histopathological changes in a mouse model of Staphylococcus aureus-induced bloodstream infections Journal of Infection in Developing Countries Staphylococcus aureus bloodstream infections inflammation cytokines Histopathology |
| title | Inflammatory responses and histopathological changes in a mouse model of Staphylococcus aureus-induced bloodstream infections |
| title_full | Inflammatory responses and histopathological changes in a mouse model of Staphylococcus aureus-induced bloodstream infections |
| title_fullStr | Inflammatory responses and histopathological changes in a mouse model of Staphylococcus aureus-induced bloodstream infections |
| title_full_unstemmed | Inflammatory responses and histopathological changes in a mouse model of Staphylococcus aureus-induced bloodstream infections |
| title_short | Inflammatory responses and histopathological changes in a mouse model of Staphylococcus aureus-induced bloodstream infections |
| title_sort | inflammatory responses and histopathological changes in a mouse model of staphylococcus aureus induced bloodstream infections |
| topic | Staphylococcus aureus bloodstream infections inflammation cytokines Histopathology |
| url | https://jidc.org/index.php/journal/article/view/7800 |
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