Delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model.

<h4>Objective</h4>Remote Ischemic Postconditioning (RIPC) is a promising therapeutic intervention wherein a sub-lethal ischemic insult induced in one organ (limb) improves ischemia in an organ distant to it (brain). The main objective of this study was to investigate the long-term functi...

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Main Authors: Pradilka N Drunalini Perera, Qin Hu, Junjia Tang, Li Li, Margaret Barnhart, Desislava M Doycheva, John H Zhang, Jiping Tang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090258&type=printable
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author Pradilka N Drunalini Perera
Qin Hu
Junjia Tang
Li Li
Margaret Barnhart
Desislava M Doycheva
John H Zhang
Jiping Tang
author_facet Pradilka N Drunalini Perera
Qin Hu
Junjia Tang
Li Li
Margaret Barnhart
Desislava M Doycheva
John H Zhang
Jiping Tang
author_sort Pradilka N Drunalini Perera
collection DOAJ
description <h4>Objective</h4>Remote Ischemic Postconditioning (RIPC) is a promising therapeutic intervention wherein a sub-lethal ischemic insult induced in one organ (limb) improves ischemia in an organ distant to it (brain). The main objective of this study was to investigate the long-term functional effects of delayed RIPC in a neonatal hypoxia-ischemia (HI) rat model.<h4>Method</h4>10 day old rat pups were subjected to delayed RIPC treatment and randomized into four groups: 1) Sham, 2) HI induced, 3) HI +24 hr delayed RIPC, and 4) HI +24 hr delayed RIPC with three consecutive daily treatments. Neurobehavioral tests, brain weights, gross and microscopic brain tissue morphologies, and systemic organ weights were evaluated at five weeks post surgery.<h4>Results</h4>HI induced rats performed significantly worse than sham but both groups of delayed RIPC treatment showed improvement of sensory motor functions. Furthermore, compared to the HI induced group, the delayed RIPC treatment groups showed no further detrimental changes on brain tissue, both grossly and morphologically, and no changes on the systemic organ weights.<h4>Conclusion</h4>Delayed RIPC significantly improves long term sensory motor deficits in a neonatal HI rat model. A 24 hr delayed treatment does not significantly attenuate morphological brain injury but does attenuate sensory motor deficits. Sensory motor deficits improve with both a single treatment and with three consecutive daily treatments, and the consecutive treatments are possibly being more beneficial.
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spelling doaj-art-cf0529b512b442bcba5a8ee844ce19122025-08-20T02:15:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e9025810.1371/journal.pone.0090258Delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model.Pradilka N Drunalini PereraQin HuJunjia TangLi LiMargaret BarnhartDesislava M DoychevaJohn H ZhangJiping Tang<h4>Objective</h4>Remote Ischemic Postconditioning (RIPC) is a promising therapeutic intervention wherein a sub-lethal ischemic insult induced in one organ (limb) improves ischemia in an organ distant to it (brain). The main objective of this study was to investigate the long-term functional effects of delayed RIPC in a neonatal hypoxia-ischemia (HI) rat model.<h4>Method</h4>10 day old rat pups were subjected to delayed RIPC treatment and randomized into four groups: 1) Sham, 2) HI induced, 3) HI +24 hr delayed RIPC, and 4) HI +24 hr delayed RIPC with three consecutive daily treatments. Neurobehavioral tests, brain weights, gross and microscopic brain tissue morphologies, and systemic organ weights were evaluated at five weeks post surgery.<h4>Results</h4>HI induced rats performed significantly worse than sham but both groups of delayed RIPC treatment showed improvement of sensory motor functions. Furthermore, compared to the HI induced group, the delayed RIPC treatment groups showed no further detrimental changes on brain tissue, both grossly and morphologically, and no changes on the systemic organ weights.<h4>Conclusion</h4>Delayed RIPC significantly improves long term sensory motor deficits in a neonatal HI rat model. A 24 hr delayed treatment does not significantly attenuate morphological brain injury but does attenuate sensory motor deficits. Sensory motor deficits improve with both a single treatment and with three consecutive daily treatments, and the consecutive treatments are possibly being more beneficial.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090258&type=printable
spellingShingle Pradilka N Drunalini Perera
Qin Hu
Junjia Tang
Li Li
Margaret Barnhart
Desislava M Doycheva
John H Zhang
Jiping Tang
Delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model.
PLoS ONE
title Delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model.
title_full Delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model.
title_fullStr Delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model.
title_full_unstemmed Delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model.
title_short Delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model.
title_sort delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090258&type=printable
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