Mesenchymal stromal cells from people with osteoporosis are fewer, and defective in both osteogenic and adipogenic capacity
Aim: Osteoporosis (OP) is caused by imbalanced bone remodelling homeostasis. It is highly prevalent, especially in post-menopausal women, resulting in high risk of fracture and morbidity. Mesenchymal stromal cells (MSCs) are osteoblast progenitors, and orchestrate the function of surrounding cells i...
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| Format: | Article |
| Language: | English |
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Open Exploration Publishing Inc.
2024-06-01
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| Series: | Exploration of Musculoskeletal Diseases |
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| Online Access: | https://www.explorationpub.com/uploads/Article/A100746/100746.pdf |
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| author | Féaron C. Cassidy Ciara Shortiss Kerry Thompson Ana Soriano Arroquia Colin G. Murphy Stephen R. Kearns William Curtin Katarzyna Goljanek-Whysall Timothy O’Brien Cynthia M. Coleman |
| author_facet | Féaron C. Cassidy Ciara Shortiss Kerry Thompson Ana Soriano Arroquia Colin G. Murphy Stephen R. Kearns William Curtin Katarzyna Goljanek-Whysall Timothy O’Brien Cynthia M. Coleman |
| author_sort | Féaron C. Cassidy |
| collection | DOAJ |
| description | Aim: Osteoporosis (OP) is caused by imbalanced bone remodelling homeostasis. It is highly prevalent, especially in post-menopausal women, resulting in high risk of fracture and morbidity. Mesenchymal stromal cells (MSCs) are osteoblast progenitors, and orchestrate the function of surrounding cells including osteoblasts. Understanding MSC phenotype and function is therefore critical in discerning the aetiology of OP and developing superior therapies. Currently, adequate long-term therapeutic strategies are not available. Methods: Bioinformatic analysis of ribonucleic acid sequencing (RNA-seq) data revealed differential expression of genes primarily related to osteogenic differentiation and proliferation, followed by confirmatory in vitro analysis. Results: This study identified novel and previously proposed targets for therapeutic intervention in OP. Functional assessment demonstrated reduced MSC number and osteogenic capacity associated with OP. Proliferation was not affected but OP was unexpectedly associated with a reduction in MSC adipogenic differentiation capacity, correlating with donor age. Conclusions: These data indicate specific targets for further studies of future treatments for OP, including the assessment of modified MSCs as therapeutics. Advances in this area may contribute to reducing fracture-associated morbidity and mortality, and improving quality of life for the 200 million people living with OP globally. |
| format | Article |
| id | doaj-art-cf008f6ffae74bf68e3eddaaaacf7ead |
| institution | Kabale University |
| issn | 2836-6468 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Open Exploration Publishing Inc. |
| record_format | Article |
| series | Exploration of Musculoskeletal Diseases |
| spelling | doaj-art-cf008f6ffae74bf68e3eddaaaacf7ead2025-08-20T03:58:40ZengOpen Exploration Publishing Inc.Exploration of Musculoskeletal Diseases2836-64682024-06-012316418010.37349/emd.2024.00046Mesenchymal stromal cells from people with osteoporosis are fewer, and defective in both osteogenic and adipogenic capacityFéaron C. Cassidy0https://orcid.org/0000-0003-2577-9519Ciara Shortiss1https://orcid.org/0000-0002-2936-3310Kerry Thompson2https://orcid.org/0000-0003-2721-8977Ana Soriano Arroquia3https://orcid.org/0000-0003-2331-0899Colin G. Murphy4https://orcid.org/0000-0003-4533-8120Stephen R. Kearns5https://orcid.org/0000-0003-2355-739XWilliam Curtin6Katarzyna Goljanek-Whysall7https://orcid.org/0000-0001-8166-8800Timothy O’Brien8https://orcid.org/0000-0001-9028-5481Cynthia M. Coleman9https://orcid.org/0000-0003-0599-3948School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland, D02 YN77 Dublin, IrelandCollege of Medicine, Nursing and Health Science, School of Medicine, Regenerative Medicine Institute, University of Galway, H91 FD82 Galway, IrelandCollege of Medicine, Nursing and Health Science, School of Medicine, Anatomy Imaging and Microscopy, University of Galway, H91 W5P7 Galway, IrelandInstitute of Ageing and Chronic Disease, University of Liverpool, L69 3BX Liverpool, UK; Institute of Pharmacology and Toxicology, University Hospital, University of Bonn, Bonn, GermanyDepartment of Trauma and Orthopaedics, Galway University Hospitals, H91 YR71 Galway, IrelandDepartment of Trauma and Orthopaedics, Galway University Hospitals, H91 YR71 Galway, IrelandDepartment of Trauma and Orthopaedics, Galway University Hospitals, H91 YR71 Galway, IrelandInstitute of Ageing and Chronic Disease, University of Liverpool, L69 3BX Liverpool, UK; Department of Physiology, School of Medicine, University of Galway, H91 TK33 Galway, IrelandCollege of Medicine, Nursing and Health Science, School of Medicine, Regenerative Medicine Institute, University of Galway, H91 FD82 Galway, Ireland; Saolta University Healthcare Group, Galway University Hospitals, H91 YR71 Galway, Ireland; CÚRAM Centre for Research in Medical Devices, College of Medicine, Nursing and Health Sciences, School of Medicine, University of Galway, H91 FD82 Galway, IrelandCollege of Medicine, Nursing and Health Science, School of Medicine, Regenerative Medicine Institute, University of Galway, H91 FD82 Galway, IrelandAim: Osteoporosis (OP) is caused by imbalanced bone remodelling homeostasis. It is highly prevalent, especially in post-menopausal women, resulting in high risk of fracture and morbidity. Mesenchymal stromal cells (MSCs) are osteoblast progenitors, and orchestrate the function of surrounding cells including osteoblasts. Understanding MSC phenotype and function is therefore critical in discerning the aetiology of OP and developing superior therapies. Currently, adequate long-term therapeutic strategies are not available. Methods: Bioinformatic analysis of ribonucleic acid sequencing (RNA-seq) data revealed differential expression of genes primarily related to osteogenic differentiation and proliferation, followed by confirmatory in vitro analysis. Results: This study identified novel and previously proposed targets for therapeutic intervention in OP. Functional assessment demonstrated reduced MSC number and osteogenic capacity associated with OP. Proliferation was not affected but OP was unexpectedly associated with a reduction in MSC adipogenic differentiation capacity, correlating with donor age. Conclusions: These data indicate specific targets for further studies of future treatments for OP, including the assessment of modified MSCs as therapeutics. Advances in this area may contribute to reducing fracture-associated morbidity and mortality, and improving quality of life for the 200 million people living with OP globally.https://www.explorationpub.com/uploads/Article/A100746/100746.pdfosteoporosismesenchymal stromal cellmesenchymal stem cellbone marrow stromal cellsosteogenesisdifferentiation |
| spellingShingle | Féaron C. Cassidy Ciara Shortiss Kerry Thompson Ana Soriano Arroquia Colin G. Murphy Stephen R. Kearns William Curtin Katarzyna Goljanek-Whysall Timothy O’Brien Cynthia M. Coleman Mesenchymal stromal cells from people with osteoporosis are fewer, and defective in both osteogenic and adipogenic capacity Exploration of Musculoskeletal Diseases osteoporosis mesenchymal stromal cell mesenchymal stem cell bone marrow stromal cells osteogenesis differentiation |
| title | Mesenchymal stromal cells from people with osteoporosis are fewer, and defective in both osteogenic and adipogenic capacity |
| title_full | Mesenchymal stromal cells from people with osteoporosis are fewer, and defective in both osteogenic and adipogenic capacity |
| title_fullStr | Mesenchymal stromal cells from people with osteoporosis are fewer, and defective in both osteogenic and adipogenic capacity |
| title_full_unstemmed | Mesenchymal stromal cells from people with osteoporosis are fewer, and defective in both osteogenic and adipogenic capacity |
| title_short | Mesenchymal stromal cells from people with osteoporosis are fewer, and defective in both osteogenic and adipogenic capacity |
| title_sort | mesenchymal stromal cells from people with osteoporosis are fewer and defective in both osteogenic and adipogenic capacity |
| topic | osteoporosis mesenchymal stromal cell mesenchymal stem cell bone marrow stromal cells osteogenesis differentiation |
| url | https://www.explorationpub.com/uploads/Article/A100746/100746.pdf |
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