Neuroprotective effect of olive leaf extract in the mouse neuroblastoma cell line (NB2a): A good model to evaluate the neuroprotective effects of natural products
Chronic neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases, whose prevalence increases with aging, cause serious health problems with inadequate treatment options. Long-term exposure to low doses of pesticides like chlorpyrifos has been linked to an increased...
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Society of Turaz Bilim
2024-04-01
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| Series: | Medicine Science |
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| Online Access: | https://www.medicinescience.org/?mno=211330 |
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| author | Ercument Olmez Ertan Dariverenli Kamil Vural Tugce Fafal Dilek Gulce Tatli Bijen Kivcak |
| author_facet | Ercument Olmez Ertan Dariverenli Kamil Vural Tugce Fafal Dilek Gulce Tatli Bijen Kivcak |
| author_sort | Ercument Olmez |
| collection | DOAJ |
| description | Chronic neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases, whose prevalence increases with aging, cause serious health problems with inadequate treatment options. Long-term exposure to low doses of pesticides like chlorpyrifos has been linked to an increased risk of developing neurodegenerative diseases. This study assessed the effects of olive leaf extract (OLE) on cell viability and neurite outgrowth inhibition induced by chlorpyrifos (25 µM) using the mouse NB2a neuroblastoma cell line. High concentrations of OLE as 100 to 3000 µg/ml reduced cell viability, likely due to its anticancer properties. On the other hand, lower concentrations of OLE as 3, 10, 30 and 100 µg/ml significantly reversed the inhibition of neurite outgrowth caused by chlorpyrifos exposure, demonstrating a neuroprotective effect. In conclusion, the regular consumption of OLE may effectively slow or prevent the advancement of neurodegenerative diseases linked to long-term exposure to low-dose organophosphate pesticides. Furthermore, the chlorpyrifos-induced moderate neurotoxicity model can be employed as a valuable screening method for evaluating the neuroprotective potential of natural products, including various plant extracts. [Med-Science 2024; 13(4.000): 796-802] |
| format | Article |
| id | doaj-art-cefdd606e71f4e36b08a4b09ef6f266d |
| institution | Kabale University |
| issn | 2147-0634 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Society of Turaz Bilim |
| record_format | Article |
| series | Medicine Science |
| spelling | doaj-art-cefdd606e71f4e36b08a4b09ef6f266d2025-02-07T08:49:23ZengSociety of Turaz BilimMedicine Science2147-06342024-04-0113479680210.5455/medscience.2024.07.082211330Neuroprotective effect of olive leaf extract in the mouse neuroblastoma cell line (NB2a): A good model to evaluate the neuroprotective effects of natural productsErcument Olmez0Ertan Dariverenli1Kamil Vural2Tugce Fafal3Dilek Gulce Tatli4Bijen Kivcak5Manisa Celal Bayar University, Faculty of Medicine, Department of Medical Pharmacology, Manisa, Turkiye Manisa Celal Bayar University, Faculty of Medicine, Department of Medical Pharmacology, Manisa, Turkiye Manisa Celal Bayar University, Faculty of Medicine, Department of Medical Pharmacology, Manisa, Turkiye Ege University, Faculty of Pharmacy, Department of Pharmacognosy, Izmir, Turkiye Manisa Celal Bayar University, Faculty of Medicine, Department of Medical Pharmacology, Manisa, Turkiye Ege University, Faculty of Pharmacy, Department of Pharmacognosy, Izmir, TurkiyeChronic neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases, whose prevalence increases with aging, cause serious health problems with inadequate treatment options. Long-term exposure to low doses of pesticides like chlorpyrifos has been linked to an increased risk of developing neurodegenerative diseases. This study assessed the effects of olive leaf extract (OLE) on cell viability and neurite outgrowth inhibition induced by chlorpyrifos (25 µM) using the mouse NB2a neuroblastoma cell line. High concentrations of OLE as 100 to 3000 µg/ml reduced cell viability, likely due to its anticancer properties. On the other hand, lower concentrations of OLE as 3, 10, 30 and 100 µg/ml significantly reversed the inhibition of neurite outgrowth caused by chlorpyrifos exposure, demonstrating a neuroprotective effect. In conclusion, the regular consumption of OLE may effectively slow or prevent the advancement of neurodegenerative diseases linked to long-term exposure to low-dose organophosphate pesticides. Furthermore, the chlorpyrifos-induced moderate neurotoxicity model can be employed as a valuable screening method for evaluating the neuroprotective potential of natural products, including various plant extracts. [Med-Science 2024; 13(4.000): 796-802]https://www.medicinescience.org/?mno=211330neurodegenerative diseasesolive leaf extractchlorpyrifosneurite outgrowth |
| spellingShingle | Ercument Olmez Ertan Dariverenli Kamil Vural Tugce Fafal Dilek Gulce Tatli Bijen Kivcak Neuroprotective effect of olive leaf extract in the mouse neuroblastoma cell line (NB2a): A good model to evaluate the neuroprotective effects of natural products Medicine Science neurodegenerative diseases olive leaf extract chlorpyrifos neurite outgrowth |
| title | Neuroprotective effect of olive leaf extract in the mouse neuroblastoma cell line (NB2a): A good model to evaluate the neuroprotective effects of natural products |
| title_full | Neuroprotective effect of olive leaf extract in the mouse neuroblastoma cell line (NB2a): A good model to evaluate the neuroprotective effects of natural products |
| title_fullStr | Neuroprotective effect of olive leaf extract in the mouse neuroblastoma cell line (NB2a): A good model to evaluate the neuroprotective effects of natural products |
| title_full_unstemmed | Neuroprotective effect of olive leaf extract in the mouse neuroblastoma cell line (NB2a): A good model to evaluate the neuroprotective effects of natural products |
| title_short | Neuroprotective effect of olive leaf extract in the mouse neuroblastoma cell line (NB2a): A good model to evaluate the neuroprotective effects of natural products |
| title_sort | neuroprotective effect of olive leaf extract in the mouse neuroblastoma cell line nb2a a good model to evaluate the neuroprotective effects of natural products |
| topic | neurodegenerative diseases olive leaf extract chlorpyrifos neurite outgrowth |
| url | https://www.medicinescience.org/?mno=211330 |
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