Raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulants

Abstract Colonization of the Moon and other planets is an aspiration of NASA and may yield important benefits for our civilization. The feasibility of such endeavors depends on both innovative engineering concepts and the successful adaptation of life forms that exist on Earth to inhospitable enviro...

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Main Authors: Axell Rodriguez, Borja Barbero Barcenilla, Emily Hall, Ishan Kundel, Alexander Meyers, Sarah Wyatt, Dorothy Shippen, Dmitry Kurouski
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:npj Microgravity
Online Access:https://doi.org/10.1038/s41526-025-00479-8
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author Axell Rodriguez
Borja Barbero Barcenilla
Emily Hall
Ishan Kundel
Alexander Meyers
Sarah Wyatt
Dorothy Shippen
Dmitry Kurouski
author_facet Axell Rodriguez
Borja Barbero Barcenilla
Emily Hall
Ishan Kundel
Alexander Meyers
Sarah Wyatt
Dorothy Shippen
Dmitry Kurouski
author_sort Axell Rodriguez
collection DOAJ
description Abstract Colonization of the Moon and other planets is an aspiration of NASA and may yield important benefits for our civilization. The feasibility of such endeavors depends on both innovative engineering concepts and the successful adaptation of life forms that exist on Earth to inhospitable environments. In this study, we investigate the potential of Raman spectroscopy (RS) in a non-invasive and non-destructive assessment of changes in the biochemistry of plants exposed to zero gravity on the International Space Station and during growth on lunar regolith simulants on Earth. We report that RS can sense changes in plant carotenoids, pectin, cellulose, and phenolics, which in turn, could be used to gauge the degree of plant stress in new environments. Our findings also demonstrate that RS can monitor the efficiency of soil supplements that can be used to mitigate nutrient-free regolith media. We conclude that RS can serve as a highly efficient approach for monitoring plant health in exotic environments.
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spelling doaj-art-cef88c0c75b24d04bdc446ed012b4dfb2025-08-20T02:03:36ZengNature Portfolionpj Microgravity2373-80652025-05-011111810.1038/s41526-025-00479-8Raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulantsAxell Rodriguez0Borja Barbero Barcenilla1Emily Hall2Ishan Kundel3Alexander Meyers4Sarah Wyatt5Dorothy Shippen6Dmitry Kurouski7Department of Biochemistry and Biophysics, Texas A&M UniversityDepartment of Biochemistry and Biophysics, Texas A&M UniversityDepartment of Biochemistry and Biophysics, Texas A&M UniversityDepartment of Biochemistry and Biophysics, Texas A&M UniversityNASA Postdoctoral Program, Oak Ridge Associated Universities, Kennedy Space Center FLMolecular and Cellular Biology Program, Ohio UniversityDepartment of Biochemistry and Biophysics, Texas A&M UniversityDepartment of Biochemistry and Biophysics, Texas A&M UniversityAbstract Colonization of the Moon and other planets is an aspiration of NASA and may yield important benefits for our civilization. The feasibility of such endeavors depends on both innovative engineering concepts and the successful adaptation of life forms that exist on Earth to inhospitable environments. In this study, we investigate the potential of Raman spectroscopy (RS) in a non-invasive and non-destructive assessment of changes in the biochemistry of plants exposed to zero gravity on the International Space Station and during growth on lunar regolith simulants on Earth. We report that RS can sense changes in plant carotenoids, pectin, cellulose, and phenolics, which in turn, could be used to gauge the degree of plant stress in new environments. Our findings also demonstrate that RS can monitor the efficiency of soil supplements that can be used to mitigate nutrient-free regolith media. We conclude that RS can serve as a highly efficient approach for monitoring plant health in exotic environments.https://doi.org/10.1038/s41526-025-00479-8
spellingShingle Axell Rodriguez
Borja Barbero Barcenilla
Emily Hall
Ishan Kundel
Alexander Meyers
Sarah Wyatt
Dorothy Shippen
Dmitry Kurouski
Raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulants
npj Microgravity
title Raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulants
title_full Raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulants
title_fullStr Raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulants
title_full_unstemmed Raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulants
title_short Raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulants
title_sort raman spectroscopy as a tool for assessing plant growth in space and on lunar regolith simulants
url https://doi.org/10.1038/s41526-025-00479-8
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