Dietary quercetin improves growth performance and modulates non-specific immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling pathways in black carp (Mylopharyngodon piceus) fed high-fat diets
This study explored how quercetin affected growth, innate immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling molecules in juvenile black carp fed high-fat diets (HFDs). A total of 540 healthy fish (10.53 ± 0.35 g) were randomly divided into 6 groups with 3 repli...
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425002959 |
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| author | Jianhua Ming Jinglong Chen Fangjie Zheng Ting Wang Youhai Du Jiahui Wang Xianping Shao Xia Yang Chenglong Wu Jinyun Ye |
| author_facet | Jianhua Ming Jinglong Chen Fangjie Zheng Ting Wang Youhai Du Jiahui Wang Xianping Shao Xia Yang Chenglong Wu Jinyun Ye |
| author_sort | Jianhua Ming |
| collection | DOAJ |
| description | This study explored how quercetin affected growth, innate immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling molecules in juvenile black carp fed high-fat diets (HFDs). A total of 540 healthy fish (10.53 ± 0.35 g) were randomly divided into 6 groups with 3 replicates each and fed a normal-fat diet with 5 % fat (NFD), a HFD with 15 % fat, and 4 HFDs supplemented with quercetin at different doses for 8 weeks, respectively. The results revealed that, compared to the NFD group, the HFD group significantly reduced growth and immune parameters (P < 0.05), leading to inflammatory response, oxidative stress, lipid metabolism disorder, and hepatic histopathological symptoms. However, compared to the HFD group, 1) Supplementing 600 mg/kg quercetin in HFD increased WGR, SGR, and SR, while reducing FCR (P < 0.05); 2) HFD supplemented with 600 and 900 mg/kg quercetin improved hepatic histopathological abnormalities, enhanced serum lysozyme, ACP, and ALP activities, and upregulated mRNA expression levels of liver immune genes and anti-inflammatory cytokines, while downregulating pro-inflammatory cytokines mRNA expression (P < 0.05); 3) Adding 600 and 900 mg/kg quercetin to HFDs boosted liver antioxidant enzyme activities and upregulated mRNA expression levels of these antioxidant enzymes and nrf2, while inhibiting keap1a and keap1b mRNA levels (P < 0.05); 4) Supplementing 600 and 900 mg/kg quercetin in HFDs reduced serum lipid content and upregulated mRNA expression levels of AMPK subunits and lipolysis genes, while downregulating mRNA expression levels of lipogenesis genes in the liver of black carp (P < 0.05). Therefore, supplementing quercetin at 600 and 900 mg/kg in HFDs improved growth, enhanced immunity and antioxidant capacity, promoted lipid metabolism, alleviated inflammatory response and oxidative stress damage in fish by inhibiting NF-κB or activating Nrf2 and AMPK signaling pathways. Based on binomial regression analysis of WGR, the optimal quercetin supplemental level in high-fat diets for juvenile black carp was 620.50 mg/kg. |
| format | Article |
| id | doaj-art-cef2a0ab0f3446bf98234c3ce5f43fba |
| institution | DOAJ |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-cef2a0ab0f3446bf98234c3ce5f43fba2025-08-20T02:57:08ZengElsevierAquaculture Reports2352-51342025-09-014310290910.1016/j.aqrep.2025.102909Dietary quercetin improves growth performance and modulates non-specific immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling pathways in black carp (Mylopharyngodon piceus) fed high-fat dietsJianhua Ming0Jinglong Chen1Fangjie Zheng2Ting Wang3Youhai Du4Jiahui Wang5Xianping Shao6Xia Yang7Chenglong Wu8Jinyun Ye9Corresponding authors.; Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaZhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaZhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaZhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaZhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaZhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaZhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaZhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaCorresponding authors.; Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaZhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, ChinaThis study explored how quercetin affected growth, innate immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling molecules in juvenile black carp fed high-fat diets (HFDs). A total of 540 healthy fish (10.53 ± 0.35 g) were randomly divided into 6 groups with 3 replicates each and fed a normal-fat diet with 5 % fat (NFD), a HFD with 15 % fat, and 4 HFDs supplemented with quercetin at different doses for 8 weeks, respectively. The results revealed that, compared to the NFD group, the HFD group significantly reduced growth and immune parameters (P < 0.05), leading to inflammatory response, oxidative stress, lipid metabolism disorder, and hepatic histopathological symptoms. However, compared to the HFD group, 1) Supplementing 600 mg/kg quercetin in HFD increased WGR, SGR, and SR, while reducing FCR (P < 0.05); 2) HFD supplemented with 600 and 900 mg/kg quercetin improved hepatic histopathological abnormalities, enhanced serum lysozyme, ACP, and ALP activities, and upregulated mRNA expression levels of liver immune genes and anti-inflammatory cytokines, while downregulating pro-inflammatory cytokines mRNA expression (P < 0.05); 3) Adding 600 and 900 mg/kg quercetin to HFDs boosted liver antioxidant enzyme activities and upregulated mRNA expression levels of these antioxidant enzymes and nrf2, while inhibiting keap1a and keap1b mRNA levels (P < 0.05); 4) Supplementing 600 and 900 mg/kg quercetin in HFDs reduced serum lipid content and upregulated mRNA expression levels of AMPK subunits and lipolysis genes, while downregulating mRNA expression levels of lipogenesis genes in the liver of black carp (P < 0.05). Therefore, supplementing quercetin at 600 and 900 mg/kg in HFDs improved growth, enhanced immunity and antioxidant capacity, promoted lipid metabolism, alleviated inflammatory response and oxidative stress damage in fish by inhibiting NF-κB or activating Nrf2 and AMPK signaling pathways. Based on binomial regression analysis of WGR, the optimal quercetin supplemental level in high-fat diets for juvenile black carp was 620.50 mg/kg.http://www.sciencedirect.com/science/article/pii/S2352513425002959Mylopharyngodon piceusQuercetinNon-specific immunityAntioxidant capacityLipid metabolism |
| spellingShingle | Jianhua Ming Jinglong Chen Fangjie Zheng Ting Wang Youhai Du Jiahui Wang Xianping Shao Xia Yang Chenglong Wu Jinyun Ye Dietary quercetin improves growth performance and modulates non-specific immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling pathways in black carp (Mylopharyngodon piceus) fed high-fat diets Aquaculture Reports Mylopharyngodon piceus Quercetin Non-specific immunity Antioxidant capacity Lipid metabolism |
| title | Dietary quercetin improves growth performance and modulates non-specific immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling pathways in black carp (Mylopharyngodon piceus) fed high-fat diets |
| title_full | Dietary quercetin improves growth performance and modulates non-specific immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling pathways in black carp (Mylopharyngodon piceus) fed high-fat diets |
| title_fullStr | Dietary quercetin improves growth performance and modulates non-specific immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling pathways in black carp (Mylopharyngodon piceus) fed high-fat diets |
| title_full_unstemmed | Dietary quercetin improves growth performance and modulates non-specific immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling pathways in black carp (Mylopharyngodon piceus) fed high-fat diets |
| title_short | Dietary quercetin improves growth performance and modulates non-specific immunity, antioxidant capacity, and lipid metabolism via NF-κB, Nrf2, and AMPK signaling pathways in black carp (Mylopharyngodon piceus) fed high-fat diets |
| title_sort | dietary quercetin improves growth performance and modulates non specific immunity antioxidant capacity and lipid metabolism via nf κb nrf2 and ampk signaling pathways in black carp mylopharyngodon piceus fed high fat diets |
| topic | Mylopharyngodon piceus Quercetin Non-specific immunity Antioxidant capacity Lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425002959 |
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