Extraction Variables Optimization on Phenolics Content and Antioxidant Activities of in-vitro Propagated Leaves of Curcuma caesia Roxb. using Response Surface Methodology
Curcuma caesia Roxb. (Zingiberaceae) is one of the endangered species in the genus Curcuma that possesses numerous beneficial bioactive compounds that are responsible for various pharmacological properties including anti-cancer, anti-asthmatic, anti-diabetic, and others. In the past few years, the n...
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North Carolina State University
2025-04-01
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| author | Zainol Haida Sharin Ab Ghani Mansor Hakiman |
| author_facet | Zainol Haida Sharin Ab Ghani Mansor Hakiman |
| author_sort | Zainol Haida |
| collection | DOAJ |
| description | Curcuma caesia Roxb. (Zingiberaceae) is one of the endangered species in the genus Curcuma that possesses numerous beneficial bioactive compounds that are responsible for various pharmacological properties including anti-cancer, anti-asthmatic, anti-diabetic, and others. In the past few years, the number of C. caesia plants was reported to have drastically decreased due to over-harvesting activity. To conserve this species, plant tissue culture method was used as a propagation tool for the mass production of C. caesia plantlets. Hence, this study aimed to optimize the extraction of phenolics content and antioxidant activities using response surface methodology. In this study, central composite design (CCD) was used to investigate the effects of three independent variables, namely solvent-solid ratio (mL/g), methanol concentration (%), and extraction temperature (°C), on phenolics content and antioxidant activities. Based on the results, the optimal extraction condition was achieved using 54.02 mL/g solvent-solid ratio, 70% methanol concentration, and 70 °C of extraction temperature. |
| format | Article |
| id | doaj-art-ceee7aae58d84c7093869d4d3a8041a5 |
| institution | DOAJ |
| issn | 1930-2126 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj-art-ceee7aae58d84c7093869d4d3a8041a52025-08-20T03:11:50ZengNorth Carolina State UniversityBioResources1930-21262025-04-01202459046072913Extraction Variables Optimization on Phenolics Content and Antioxidant Activities of in-vitro Propagated Leaves of Curcuma caesia Roxb. using Response Surface MethodologyZainol Haida0Sharin Ab Ghani1Mansor Hakiman2Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Laboratory of Sustainable Resources Management, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaHigh Voltage Engineering Research Laboratory, Faculty of Electrical Engineering, Universiti Teknikal Malaysia, Melaka, Hang Tuah Jaya, 76100 Durian Tunggal, Melaka, MalaysiaDepartment of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Laboratory of Sustainable Resources Management, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaCurcuma caesia Roxb. (Zingiberaceae) is one of the endangered species in the genus Curcuma that possesses numerous beneficial bioactive compounds that are responsible for various pharmacological properties including anti-cancer, anti-asthmatic, anti-diabetic, and others. In the past few years, the number of C. caesia plants was reported to have drastically decreased due to over-harvesting activity. To conserve this species, plant tissue culture method was used as a propagation tool for the mass production of C. caesia plantlets. Hence, this study aimed to optimize the extraction of phenolics content and antioxidant activities using response surface methodology. In this study, central composite design (CCD) was used to investigate the effects of three independent variables, namely solvent-solid ratio (mL/g), methanol concentration (%), and extraction temperature (°C), on phenolics content and antioxidant activities. Based on the results, the optimal extraction condition was achieved using 54.02 mL/g solvent-solid ratio, 70% methanol concentration, and 70 °C of extraction temperature.https://ojs.bioresources.com/index.php/BRJ/article/view/24608black turmericextraction variablesphenolics contentantioxidant activities |
| spellingShingle | Zainol Haida Sharin Ab Ghani Mansor Hakiman Extraction Variables Optimization on Phenolics Content and Antioxidant Activities of in-vitro Propagated Leaves of Curcuma caesia Roxb. using Response Surface Methodology BioResources black turmeric extraction variables phenolics content antioxidant activities |
| title | Extraction Variables Optimization on Phenolics Content and Antioxidant Activities of in-vitro Propagated Leaves of Curcuma caesia Roxb. using Response Surface Methodology |
| title_full | Extraction Variables Optimization on Phenolics Content and Antioxidant Activities of in-vitro Propagated Leaves of Curcuma caesia Roxb. using Response Surface Methodology |
| title_fullStr | Extraction Variables Optimization on Phenolics Content and Antioxidant Activities of in-vitro Propagated Leaves of Curcuma caesia Roxb. using Response Surface Methodology |
| title_full_unstemmed | Extraction Variables Optimization on Phenolics Content and Antioxidant Activities of in-vitro Propagated Leaves of Curcuma caesia Roxb. using Response Surface Methodology |
| title_short | Extraction Variables Optimization on Phenolics Content and Antioxidant Activities of in-vitro Propagated Leaves of Curcuma caesia Roxb. using Response Surface Methodology |
| title_sort | extraction variables optimization on phenolics content and antioxidant activities of in vitro propagated leaves of curcuma caesia roxb using response surface methodology |
| topic | black turmeric extraction variables phenolics content antioxidant activities |
| url | https://ojs.bioresources.com/index.php/BRJ/article/view/24608 |
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