Chromatin Regulation in Development: Current Understanding and Approaches
The regulation of mammalian stem cell fate during differentiation is complex and can be delineated across many levels. At the chromatin level, the replacement of histone variants by chromatin-modifying proteins, enrichment of specific active and repressive histone modifications, long-range gene inte...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/8817581 |
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| _version_ | 1849683684818419712 |
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| author | Zi Hao Zheng Tsz Wing Sam YingYing Zeng Justin Jang Hann Chu Yuin-Han Loh |
| author_facet | Zi Hao Zheng Tsz Wing Sam YingYing Zeng Justin Jang Hann Chu Yuin-Han Loh |
| author_sort | Zi Hao Zheng |
| collection | DOAJ |
| description | The regulation of mammalian stem cell fate during differentiation is complex and can be delineated across many levels. At the chromatin level, the replacement of histone variants by chromatin-modifying proteins, enrichment of specific active and repressive histone modifications, long-range gene interactions, and topological changes all play crucial roles in the determination of cell fate. These processes control regulatory elements of critical transcriptional factors, thereby establishing the networks unique to different cell fates and initiate waves of distinctive transcription events. Due to the technical challenges posed by previous methods, it was difficult to decipher the mechanism of cell fate determination at early embryogenesis through chromatin regulation. Recently, single-cell approaches have revolutionised the field of developmental biology, allowing unprecedented insights into chromatin structure and interactions in early lineage segregation events during differentiation. Here, we review the recent technological advancements and how they have furthered our understanding of chromatin regulation during early differentiation events. |
| format | Article |
| id | doaj-art-cedb79554f0d4fcea39485fe6cfa72ee |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-cedb79554f0d4fcea39485fe6cfa72ee2025-08-20T03:23:43ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/88175818817581Chromatin Regulation in Development: Current Understanding and ApproachesZi Hao Zheng0Tsz Wing Sam1YingYing Zeng2Justin Jang Hann Chu3Yuin-Han Loh4Laboratory for Epigenetics, Stem Cells & Cell Therapy, Programme in Stem Cell, Regenerative Medicine and Aging, ASTAR Institute of Molecular and Cell Biology, 138673, SingaporeLaboratory for Epigenetics, Stem Cells & Cell Therapy, Programme in Stem Cell, Regenerative Medicine and Aging, ASTAR Institute of Molecular and Cell Biology, 138673, SingaporeLaboratory for Epigenetics, Stem Cells & Cell Therapy, Programme in Stem Cell, Regenerative Medicine and Aging, ASTAR Institute of Molecular and Cell Biology, 138673, SingaporeLaboratory of Molecular RNA Virology and Antiviral Strategies, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, MD4 Level 5, 5 Science Drive 2, 117597, SingaporeLaboratory for Epigenetics, Stem Cells & Cell Therapy, Programme in Stem Cell, Regenerative Medicine and Aging, ASTAR Institute of Molecular and Cell Biology, 138673, SingaporeThe regulation of mammalian stem cell fate during differentiation is complex and can be delineated across many levels. At the chromatin level, the replacement of histone variants by chromatin-modifying proteins, enrichment of specific active and repressive histone modifications, long-range gene interactions, and topological changes all play crucial roles in the determination of cell fate. These processes control regulatory elements of critical transcriptional factors, thereby establishing the networks unique to different cell fates and initiate waves of distinctive transcription events. Due to the technical challenges posed by previous methods, it was difficult to decipher the mechanism of cell fate determination at early embryogenesis through chromatin regulation. Recently, single-cell approaches have revolutionised the field of developmental biology, allowing unprecedented insights into chromatin structure and interactions in early lineage segregation events during differentiation. Here, we review the recent technological advancements and how they have furthered our understanding of chromatin regulation during early differentiation events.http://dx.doi.org/10.1155/2021/8817581 |
| spellingShingle | Zi Hao Zheng Tsz Wing Sam YingYing Zeng Justin Jang Hann Chu Yuin-Han Loh Chromatin Regulation in Development: Current Understanding and Approaches Stem Cells International |
| title | Chromatin Regulation in Development: Current Understanding and Approaches |
| title_full | Chromatin Regulation in Development: Current Understanding and Approaches |
| title_fullStr | Chromatin Regulation in Development: Current Understanding and Approaches |
| title_full_unstemmed | Chromatin Regulation in Development: Current Understanding and Approaches |
| title_short | Chromatin Regulation in Development: Current Understanding and Approaches |
| title_sort | chromatin regulation in development current understanding and approaches |
| url | http://dx.doi.org/10.1155/2021/8817581 |
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