The Effects of <i>Anthyllis vulneraria</i> Hydroalcoholic Leaf Extract as an Adjuvant in Wound Healing

<i>Anthyllis vulneraria</i> is a traditional medicinal plant with confirmed anti-inflammatory properties, attributed to its high polyphenolic content. This study aimed to evaluate the wound-healing potential of <i>A. vulneraria</i> leaf extract in a rat burn model. Four group...

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Main Authors: Olga-Maria Iova, Gheorghe-Eduard Marin, Ana-Maria Vlase, Marcela Achim, Dana Muntean, Ioan Tomuţă, Remus Moldovan, Nicoleta Decea, Bogdan Alexandru Gheban, Sebastian Romeo Pintilie, Oana-Alina Hoteiuc, Roxana Denisa Capras, Adriana Gabriela Filip
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Applied Sciences
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Online Access:https://www.mdpi.com/2076-3417/15/15/8388
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author Olga-Maria Iova
Gheorghe-Eduard Marin
Ana-Maria Vlase
Marcela Achim
Dana Muntean
Ioan Tomuţă
Remus Moldovan
Nicoleta Decea
Bogdan Alexandru Gheban
Sebastian Romeo Pintilie
Oana-Alina Hoteiuc
Roxana Denisa Capras
Adriana Gabriela Filip
author_facet Olga-Maria Iova
Gheorghe-Eduard Marin
Ana-Maria Vlase
Marcela Achim
Dana Muntean
Ioan Tomuţă
Remus Moldovan
Nicoleta Decea
Bogdan Alexandru Gheban
Sebastian Romeo Pintilie
Oana-Alina Hoteiuc
Roxana Denisa Capras
Adriana Gabriela Filip
author_sort Olga-Maria Iova
collection DOAJ
description <i>Anthyllis vulneraria</i> is a traditional medicinal plant with confirmed anti-inflammatory properties, attributed to its high polyphenolic content. This study aimed to evaluate the wound-healing potential of <i>A. vulneraria</i> leaf extract in a rat burn model. Four groups of eight Wistar rats each received the following daily topical applications for 14 days: vehicle cream (negative control); silver sulfadiazine (positive control); or plant-based creams containing either 1 mg/cm<sup>2</sup> or 2 mg/cm<sup>2</sup> of polyphenols (experimental groups 1 and 2, respectively). On days 7 and 14, four animals per group were euthanized for histological and oxidative stress evaluations. LC-MS/MS analysis of the leaf extract identified hyperoside, ferulic acid, and <i>p</i>-coumaric acid as major constituents. Experimental group 1 showed significantly enhanced wound closure on days 5 and 7, while group 2 exhibited a significant effect on day 5. All oxidative stress markers, except catalase activity, differed significantly among the groups, with the most favorable results observed in group 2. IL-8 levels decreased after the extract treatment, while no significant microscopic changes were observed. These results indicate that <i>A. vulneraria</i> leaf extract may serve as a valuable adjuvant in burn wound healing.
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spelling doaj-art-ced40c64eb564b2398bc60eda08212512025-08-20T03:36:35ZengMDPI AGApplied Sciences2076-34172025-07-011515838810.3390/app15158388The Effects of <i>Anthyllis vulneraria</i> Hydroalcoholic Leaf Extract as an Adjuvant in Wound HealingOlga-Maria Iova0Gheorghe-Eduard Marin1Ana-Maria Vlase2Marcela Achim3Dana Muntean4Ioan Tomuţă5Remus Moldovan6Nicoleta Decea7Bogdan Alexandru Gheban8Sebastian Romeo Pintilie9Oana-Alina Hoteiuc10Roxana Denisa Capras11Adriana Gabriela Filip12Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400349 Cluj-Napoca, RomaniaFaculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400349 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 23 Gheorghe Marinescu Street, 400337 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaPhysiology Department, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400126 Cluj-Napoca, RomaniaPhysiology Department, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400126 Cluj-Napoca, RomaniaDepartment 1, Faculty of Medical Assistance and Health Sciences, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400126 Cluj-Napoca, RomaniaFaculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400349 Cluj-Napoca, RomaniaPhysiology Department, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400126 Cluj-Napoca, RomaniaDepartment of Anatomy and Embriology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Anatomy and Embriology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania<i>Anthyllis vulneraria</i> is a traditional medicinal plant with confirmed anti-inflammatory properties, attributed to its high polyphenolic content. This study aimed to evaluate the wound-healing potential of <i>A. vulneraria</i> leaf extract in a rat burn model. Four groups of eight Wistar rats each received the following daily topical applications for 14 days: vehicle cream (negative control); silver sulfadiazine (positive control); or plant-based creams containing either 1 mg/cm<sup>2</sup> or 2 mg/cm<sup>2</sup> of polyphenols (experimental groups 1 and 2, respectively). On days 7 and 14, four animals per group were euthanized for histological and oxidative stress evaluations. LC-MS/MS analysis of the leaf extract identified hyperoside, ferulic acid, and <i>p</i>-coumaric acid as major constituents. Experimental group 1 showed significantly enhanced wound closure on days 5 and 7, while group 2 exhibited a significant effect on day 5. All oxidative stress markers, except catalase activity, differed significantly among the groups, with the most favorable results observed in group 2. IL-8 levels decreased after the extract treatment, while no significant microscopic changes were observed. These results indicate that <i>A. vulneraria</i> leaf extract may serve as a valuable adjuvant in burn wound healing.https://www.mdpi.com/2076-3417/15/15/8388<i>Anthyllis vulneraria</i>polyphenolsanimal burn modelwound healing
spellingShingle Olga-Maria Iova
Gheorghe-Eduard Marin
Ana-Maria Vlase
Marcela Achim
Dana Muntean
Ioan Tomuţă
Remus Moldovan
Nicoleta Decea
Bogdan Alexandru Gheban
Sebastian Romeo Pintilie
Oana-Alina Hoteiuc
Roxana Denisa Capras
Adriana Gabriela Filip
The Effects of <i>Anthyllis vulneraria</i> Hydroalcoholic Leaf Extract as an Adjuvant in Wound Healing
Applied Sciences
<i>Anthyllis vulneraria</i>
polyphenols
animal burn model
wound healing
title The Effects of <i>Anthyllis vulneraria</i> Hydroalcoholic Leaf Extract as an Adjuvant in Wound Healing
title_full The Effects of <i>Anthyllis vulneraria</i> Hydroalcoholic Leaf Extract as an Adjuvant in Wound Healing
title_fullStr The Effects of <i>Anthyllis vulneraria</i> Hydroalcoholic Leaf Extract as an Adjuvant in Wound Healing
title_full_unstemmed The Effects of <i>Anthyllis vulneraria</i> Hydroalcoholic Leaf Extract as an Adjuvant in Wound Healing
title_short The Effects of <i>Anthyllis vulneraria</i> Hydroalcoholic Leaf Extract as an Adjuvant in Wound Healing
title_sort effects of i anthyllis vulneraria i hydroalcoholic leaf extract as an adjuvant in wound healing
topic <i>Anthyllis vulneraria</i>
polyphenols
animal burn model
wound healing
url https://www.mdpi.com/2076-3417/15/15/8388
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