Oxidative stress in neurological feline infectious peritonitis: cerebrospinal fluid 8-hydroxy-2"-deoxyguanosine and superoxide dismutase levels
Oxidative stress plays a key role in the pathogenesis of neurological disorders and viral infections affecting the central nervous system. 8-Hydroxy-2"-deoxyguanosine (8-OHdG) is a marker of oxidative DNA damage, while superoxide dismutase (SOD) reflects antioxidant defense. This study aimed to...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2025-08-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3215 |
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| author | İdil BAŞTAN Devrim İlke İRDEM Tevhide SEL Yeliz KAYA KARTAL Selahattin Halil ERGİN Arda Selin TUNÇ |
| author_facet | İdil BAŞTAN Devrim İlke İRDEM Tevhide SEL Yeliz KAYA KARTAL Selahattin Halil ERGİN Arda Selin TUNÇ |
| author_sort | İdil BAŞTAN |
| collection | DOAJ |
| description | Oxidative stress plays a key role in the pathogenesis of neurological disorders and
viral infections affecting the central nervous system. 8-Hydroxy-2"-deoxyguanosine
(8-OHdG) is a marker of oxidative DNA damage, while superoxide dismutase (SOD)
reflects antioxidant defense. This study aimed to evaluate SOD and 8-OHdG levels
in the cerebrospinal fluid (CSF) of cats with neurological feline infectious peritonitis
(FIP) to assess oxidative stress and antioxidant response. Twelve cats with neurological
FIP and 10 age-matched control cats euthanized for non-neurological conditions were
included. FIP diagnosis was confirmed by detecting feline coronavirus (FCoV) RNA in
the CSF using real-time RT-PCR and by histopathological examination. CSF samples
were analyzed for total protein, glucose, SOD, and 8-OHdG. Cats with FIP showed
significantly higher CSF protein (740±230 mg/dL) than controls (17±7 mg/dL). The
CSF/serum glucose ratio was lower in FIP cats (0.39±0.18) than in controls (0.66±0.06).
8-OHdG levels were elevated in FIP cats (6.88 ng/ml) compared to controls (1.09 ng/
ml; P<0.05). SOD levels were reduced in FIP cats (0.034±0.026 U/mg protein) versus
controls (0.312±0.136 U/mg protein; P<0.001). These findings highlight a pronounced
oxidative stress condition in neurological FIP, characterized by elevated 8-OHdG levels
and reduced SOD concentrations in the CSF. This concurrent pattern may not only serve
as a valuable biomarker of disease activity but also represent a potential therapeutic
target for antioxidant-based strategies in affected cats. |
| format | Article |
| id | doaj-art-cec9272eae9945dfa416c13917e8a448 |
| institution | Kabale University |
| issn | 1309-2251 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-cec9272eae9945dfa416c13917e8a4482025-08-20T04:03:01ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512025-08-0131454755610.9775/kvfd.2025.342513215Oxidative stress in neurological feline infectious peritonitis: cerebrospinal fluid 8-hydroxy-2"-deoxyguanosine and superoxide dismutase levelsİdil BAŞTAN0Devrim İlke İRDEM1Tevhide SEL2Yeliz KAYA KARTAL3Selahattin Halil ERGİN4Arda Selin TUNÇ5Ankara University, Faculty of Veterinary Medicine, Department of Internal Medicine, TR-06070 Ankara - TÜRKİYEAnkara University, Faculty of Veterinary Medicine, Department of Internal Medicine, TR-06070 Ankara - TÜRKİYEAnkara University, Faculty of Veterinary Medicine, Department of Biochemistry, TR-06070 Ankara - TÜRKİYEAnkara University, Faculty of Veterinary Medicine, Department of Biochemistry, TR-06070 Ankara - TÜRKİYEAnkara University, Faculty of Veterinary Medicine, Department of Veterinary Surgery, TR-06070 Ankara - TÜRKİYEAnkara University, Faculty of Veterinary Medicine, Department of Pathology, TR-06070 Ankara - TÜRKİYEOxidative stress plays a key role in the pathogenesis of neurological disorders and viral infections affecting the central nervous system. 8-Hydroxy-2"-deoxyguanosine (8-OHdG) is a marker of oxidative DNA damage, while superoxide dismutase (SOD) reflects antioxidant defense. This study aimed to evaluate SOD and 8-OHdG levels in the cerebrospinal fluid (CSF) of cats with neurological feline infectious peritonitis (FIP) to assess oxidative stress and antioxidant response. Twelve cats with neurological FIP and 10 age-matched control cats euthanized for non-neurological conditions were included. FIP diagnosis was confirmed by detecting feline coronavirus (FCoV) RNA in the CSF using real-time RT-PCR and by histopathological examination. CSF samples were analyzed for total protein, glucose, SOD, and 8-OHdG. Cats with FIP showed significantly higher CSF protein (740±230 mg/dL) than controls (17±7 mg/dL). The CSF/serum glucose ratio was lower in FIP cats (0.39±0.18) than in controls (0.66±0.06). 8-OHdG levels were elevated in FIP cats (6.88 ng/ml) compared to controls (1.09 ng/ ml; P<0.05). SOD levels were reduced in FIP cats (0.034±0.026 U/mg protein) versus controls (0.312±0.136 U/mg protein; P<0.001). These findings highlight a pronounced oxidative stress condition in neurological FIP, characterized by elevated 8-OHdG levels and reduced SOD concentrations in the CSF. This concurrent pattern may not only serve as a valuable biomarker of disease activity but also represent a potential therapeutic target for antioxidant-based strategies in affected cats.https://vetdergikafkas.org/pdf.php?id=32158-ohdgcatcerebrospinal fluidfeline infectious peritonitisoxidative stresssuperoxide dismutase |
| spellingShingle | İdil BAŞTAN Devrim İlke İRDEM Tevhide SEL Yeliz KAYA KARTAL Selahattin Halil ERGİN Arda Selin TUNÇ Oxidative stress in neurological feline infectious peritonitis: cerebrospinal fluid 8-hydroxy-2"-deoxyguanosine and superoxide dismutase levels Kafkas Universitesi Veteriner Fakültesi Dergisi 8-ohdg cat cerebrospinal fluid feline infectious peritonitis oxidative stress superoxide dismutase |
| title | Oxidative stress in neurological feline infectious peritonitis: cerebrospinal fluid 8-hydroxy-2"-deoxyguanosine and superoxide dismutase levels |
| title_full | Oxidative stress in neurological feline infectious peritonitis: cerebrospinal fluid 8-hydroxy-2"-deoxyguanosine and superoxide dismutase levels |
| title_fullStr | Oxidative stress in neurological feline infectious peritonitis: cerebrospinal fluid 8-hydroxy-2"-deoxyguanosine and superoxide dismutase levels |
| title_full_unstemmed | Oxidative stress in neurological feline infectious peritonitis: cerebrospinal fluid 8-hydroxy-2"-deoxyguanosine and superoxide dismutase levels |
| title_short | Oxidative stress in neurological feline infectious peritonitis: cerebrospinal fluid 8-hydroxy-2"-deoxyguanosine and superoxide dismutase levels |
| title_sort | oxidative stress in neurological feline infectious peritonitis cerebrospinal fluid 8 hydroxy 2 deoxyguanosine and superoxide dismutase levels |
| topic | 8-ohdg cat cerebrospinal fluid feline infectious peritonitis oxidative stress superoxide dismutase |
| url | https://vetdergikafkas.org/pdf.php?id=3215 |
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