Genetic Diversity Analysis of <i>Capsicum frutescens</i> Based on Simplified Genome Sequencing Technology
<i>Capsicum frutescens</i> (<i>C. frutescens</i>) has rich germplasm resources, but there have been no reports on its genetic diversity analysis alone using simplified genome sequencing technology (GBS). To provide a basis for the breeding of new pepper varieties and the late...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-09-01
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| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/10/9/1004 |
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| Summary: | <i>Capsicum frutescens</i> (<i>C. frutescens</i>) has rich germplasm resources, but there have been no reports on its genetic diversity analysis alone using simplified genome sequencing technology (GBS). To provide a basis for the breeding of new pepper varieties and the later development of <i>C. frutescens</i> SNP molecular markers, this study used GBS technology to sequence 65 collected pepper germplasm resources. A total of 1,399,391 SNP sites were obtained by GBS simplified genome sequencing, and there were 1,465,897 SNP variant sites. Through population genetic structure analysis, the population structure and phylogenetic tree of 65 <i>C. frutescens</i> germplasms were constructed. The GBS method is also suitable for the genetic relationship analysis of <i>C. frutescens</i>, and it also shows that there is an obvious separation of materials from different origins, and there is also a certain degree of genetic exchange. Most of <i>C. frutescens</i> varieties from Fujian Province and its surrounding areas are clustered together; <i>C. frutescens</i> varieties from western China are also clustered together. We selected T62 and T60 with a genetic distance of 0.2796 and a spiciness level of nine as the female and male parent combinations, respectively, and bred a new high-yield <i>C. frutescens</i> combination, ‘Mingjiao 308’. <i>C. frutescens</i> varieties from the same geographical origin are usually clustered together. These germplasms may contain the ancestry of multiple varieties. This result can also provide basic data for the later construction of an SNP fingerprint database. |
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| ISSN: | 2311-7524 |