Molecular detection of Methicillin-Resistant Staphylococcus aureus isolated from Subclinical Mastitis Cows in Basrah Province.
Out of 200 milk samples collected from apparently normal cows in Basrah province from 21 November 2023 to 6 February 2024, 95 (48)% were positive for the CMT test. This study used various techniques to detect the presence of staphylococcus aureus, including conventional microbiological assays (manni...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
College of Veterinary Medicine, University of Basrah, Iraq
2025-03-01
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| Series: | Basrah Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://bjvr.uobasrah.edu.iq/article_186948_63967485f0cde56d0f7a55fb4a4eb10c.pdf |
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| Summary: | Out of 200 milk samples collected from apparently normal cows in Basrah province from 21 November 2023 to 6 February 2024, 95 (48)% were positive for the CMT test. This study used various techniques to detect the presence of staphylococcus aureus, including conventional microbiological assays (mannitol salts agar and CHROMagar™ Staph aureus) and molecular methods (amplifying the nuc gene using polymerase chain reaction ). The percentage of S. aureus isolates was 38 (19 % ).From 38 ( 19 %) S. aureus isolates, 27 (71%) carried the mecA gene, 6 (16%) carried pvl, while none of the isolates carried the mecC gene. |
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| ISSN: | 1813-8497 2410-8456 |