Comparison of Activity of 10 Antibiotics Against Clinical Strains of Helicobacter pylori by Three Different Techniques

The authors determined the susceptibility of 55 single clinical strains of Helicobacter pylori isolated in the Montreal area to 10 antibiotics by three different methods - an agar dilution technique considered to be the gold standard, a disk diffusion method and the E-test. Testing was performed on...

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Main Authors: K Weiss, M Laverdiere, C Restieri
Format: Article
Language:English
Published: Wiley 1998-01-01
Series:Canadian Journal of Gastroenterology
Online Access:http://dx.doi.org/10.1155/1998/765795
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author K Weiss
M Laverdiere
C Restieri
author_facet K Weiss
M Laverdiere
C Restieri
author_sort K Weiss
collection DOAJ
description The authors determined the susceptibility of 55 single clinical strains of Helicobacter pylori isolated in the Montreal area to 10 antibiotics by three different methods - an agar dilution technique considered to be the gold standard, a disk diffusion method and the E-test. Testing was performed on Mueller-Hinton agar supplemented with 10% sheep blood; plates were incubated at 37°C for 72 h in a microaerophilic atmosphere. The metronidazole resistance rate is about 11% in the Montreal area. Macrolides are very active against H pylori isolates, with few variations in activity between older and newer molecules. Correlation among different methods was not as good as reported in the literature for metronidazole.
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spelling doaj-art-ceb2cf0cdb0e40eb902a89629086c7b12025-08-20T02:19:30ZengWileyCanadian Journal of Gastroenterology0835-79001998-01-0112318118510.1155/1998/765795Comparison of Activity of 10 Antibiotics Against Clinical Strains of Helicobacter pylori by Three Different TechniquesK Weiss0M Laverdiere1C Restieri2Department of Microbiology and Infectious Diseases, Hôpital Maisonneuve-Rosemont, University of Montreal, Montréal, Québec, CanadaDepartment of Microbiology and Infectious Diseases, Hôpital Maisonneuve-Rosemont, University of Montreal, Montréal, Québec, CanadaDepartment of Microbiology and Infectious Diseases, Hôpital Maisonneuve-Rosemont, University of Montreal, Montréal, Québec, CanadaThe authors determined the susceptibility of 55 single clinical strains of Helicobacter pylori isolated in the Montreal area to 10 antibiotics by three different methods - an agar dilution technique considered to be the gold standard, a disk diffusion method and the E-test. Testing was performed on Mueller-Hinton agar supplemented with 10% sheep blood; plates were incubated at 37°C for 72 h in a microaerophilic atmosphere. The metronidazole resistance rate is about 11% in the Montreal area. Macrolides are very active against H pylori isolates, with few variations in activity between older and newer molecules. Correlation among different methods was not as good as reported in the literature for metronidazole.http://dx.doi.org/10.1155/1998/765795
spellingShingle K Weiss
M Laverdiere
C Restieri
Comparison of Activity of 10 Antibiotics Against Clinical Strains of Helicobacter pylori by Three Different Techniques
Canadian Journal of Gastroenterology
title Comparison of Activity of 10 Antibiotics Against Clinical Strains of Helicobacter pylori by Three Different Techniques
title_full Comparison of Activity of 10 Antibiotics Against Clinical Strains of Helicobacter pylori by Three Different Techniques
title_fullStr Comparison of Activity of 10 Antibiotics Against Clinical Strains of Helicobacter pylori by Three Different Techniques
title_full_unstemmed Comparison of Activity of 10 Antibiotics Against Clinical Strains of Helicobacter pylori by Three Different Techniques
title_short Comparison of Activity of 10 Antibiotics Against Clinical Strains of Helicobacter pylori by Three Different Techniques
title_sort comparison of activity of 10 antibiotics against clinical strains of helicobacter pylori by three different techniques
url http://dx.doi.org/10.1155/1998/765795
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AT mlaverdiere comparisonofactivityof10antibioticsagainstclinicalstrainsofhelicobacterpyloribythreedifferenttechniques
AT crestieri comparisonofactivityof10antibioticsagainstclinicalstrainsofhelicobacterpyloribythreedifferenttechniques