Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead Array
As a noninvasive method, exhaled breath condensate (EBC) has gained importance to improve monitoring of lung diseases and to detect biomarkers. The aim of the study was to investigate, whether erythropoietin (EPO) is detectable in EBC. EBC was collected from 22 consecutive patients as well as from h...
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/18061 |
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| _version_ | 1832567620418666496 |
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| author | Christian Schumann Kathy Triantafilou Stefan Krueger Vinzenz Hombach Martha Triantafilou Gunther Becher Philipp M. Lepper |
| author_facet | Christian Schumann Kathy Triantafilou Stefan Krueger Vinzenz Hombach Martha Triantafilou Gunther Becher Philipp M. Lepper |
| author_sort | Christian Schumann |
| collection | DOAJ |
| description | As a noninvasive method, exhaled breath condensate (EBC) has gained importance to improve monitoring of lung diseases and to detect biomarkers. The aim of the study was to investigate, whether erythropoietin (EPO) is detectable in EBC. EBC was collected from 22 consecutive patients as well as from healthy individuals. Using a multiplex fluorescent bead
immunoassay, we detected EPO in EBC, as well as tumour necrosis factor-α (TNF-α) in 13 out of 22 patients simultaneously (EPO 0.21 ± 0.03 in U/mL and TNF-α 34.6 ± 4.2 in pg/mL, mean ± SEM). No significant differences for EPO levels or correlation between EPO and
TNF-α were found but TNF-α was significantly higher in patients with chronic obstructive pulmonary disease (COPD) than in non-COPD (obstructive sleep apnoea, OSA, and lung healthy patients). This is the first report of detection of EPO in EBC. Due to the small study size more data is needed to clarify the role of EPO in EBC. |
| format | Article |
| id | doaj-art-cea7f33cee0d456cb0ea709bf2afe454 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-cea7f33cee0d456cb0ea709bf2afe4542025-02-03T01:01:06ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/1806118061Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead ArrayChristian Schumann0Kathy Triantafilou1Stefan Krueger2Vinzenz Hombach3Martha Triantafilou4Gunther Becher5Philipp M. Lepper6Department of Internal Medicine II, Section of Pulmonary Medicine, University of Ulm, Robert-Koch-Straβe 8 89081, Ulm, GermanyInfection and Immunity Group, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UKDepartment of Internal Medicine II, Section of Pulmonary Medicine, University of Ulm, Robert-Koch-Straβe 8 89081, Ulm, GermanyDepartment of Internal Medicine II, Section of Pulmonary Medicine, University of Ulm, Robert-Koch-Straβe 8 89081, Ulm, GermanyInfection and Immunity Group, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UKResearch Society of Lung and Chest Diseases Ltd, Berlin 13125, GermanyDepartment of Internal Medicine II, Section of Pulmonary Medicine, University of Ulm, Robert-Koch-Straβe 8 89081, Ulm, GermanyAs a noninvasive method, exhaled breath condensate (EBC) has gained importance to improve monitoring of lung diseases and to detect biomarkers. The aim of the study was to investigate, whether erythropoietin (EPO) is detectable in EBC. EBC was collected from 22 consecutive patients as well as from healthy individuals. Using a multiplex fluorescent bead immunoassay, we detected EPO in EBC, as well as tumour necrosis factor-α (TNF-α) in 13 out of 22 patients simultaneously (EPO 0.21 ± 0.03 in U/mL and TNF-α 34.6 ± 4.2 in pg/mL, mean ± SEM). No significant differences for EPO levels or correlation between EPO and TNF-α were found but TNF-α was significantly higher in patients with chronic obstructive pulmonary disease (COPD) than in non-COPD (obstructive sleep apnoea, OSA, and lung healthy patients). This is the first report of detection of EPO in EBC. Due to the small study size more data is needed to clarify the role of EPO in EBC.http://dx.doi.org/10.1155/MI/2006/18061 |
| spellingShingle | Christian Schumann Kathy Triantafilou Stefan Krueger Vinzenz Hombach Martha Triantafilou Gunther Becher Philipp M. Lepper Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead Array Mediators of Inflammation |
| title | Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead Array |
| title_full | Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead Array |
| title_fullStr | Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead Array |
| title_full_unstemmed | Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead Array |
| title_short | Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead Array |
| title_sort | detection of erythropoietin in exhaled breath condensate of nonhypoxic subjects using a multiplex bead array |
| url | http://dx.doi.org/10.1155/MI/2006/18061 |
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