Mineral supplementation protected intestinal health in Litopenaeus vannamei by alleviating the immune response and oxidative damage caused by high-level of Clostridium autoethanogenum protein
This study investigates the impact of dietary mineral supplementation in Clostridium autoethanogenum protein (CAP)-containing low-fishmeal diets on growth, lipid metabolic profile, and immune and antioxidant capacity in Litopenaeus vannamei. Three diets were formulated in this experiment. The positi...
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003771 |
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| author | Jiahao Song Chaozhong Zheng Qi Hao Jiayi Huang Wencheng Huang Shuyan Chi Beiping Tan Wen Huang Shiwei Xie |
| author_facet | Jiahao Song Chaozhong Zheng Qi Hao Jiayi Huang Wencheng Huang Shuyan Chi Beiping Tan Wen Huang Shiwei Xie |
| author_sort | Jiahao Song |
| collection | DOAJ |
| description | This study investigates the impact of dietary mineral supplementation in Clostridium autoethanogenum protein (CAP)-containing low-fishmeal diets on growth, lipid metabolic profile, and immune and antioxidant capacity in Litopenaeus vannamei. Three diets were formulated in this experiment. The positive control group (PC) was a high fishmeal protein diet with 25 % fish meal, and CAP was used to replace the low fish meal diet (NC) with 50 % fish meal (12.5 % fishmeal) in the PC group. The third group was added minerals (Mg, Fe, Mn and Se) (HM) in the NC diet. Each group contained 160 shrimp, divided into 4 replicates (initial weight: 1.79 ± 0.02 g). The results showed that after fishmeal was replaced by CAP, the growth performance and antioxidant capacity of shrimp decreased. Challenges to lipid metabolism homeostasis were also observed. Transcriptional analysis revealed upregulation of innate immune genes (dorsal, relish, litaf) in the NC dietary group compared to other groups. After mineral supplementation, antioxidant and immune capacity were enhanced. In addition, correlation analysis showed that there was a significant interaction between midgut morphology and immune genes. In conclusion, mineral supplementation can partially alleviate the oxidative stress and immune imbalance caused by the replacement of fish meal with CAP, but has limited effects on the growth of shrimp. |
| format | Article |
| id | doaj-art-ce9d8ae7d62e4719945d918e18267edd |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-ce9d8ae7d62e4719945d918e18267edd2025-08-20T03:40:45ZengElsevierAquaculture Reports2352-51342025-09-014310299110.1016/j.aqrep.2025.102991Mineral supplementation protected intestinal health in Litopenaeus vannamei by alleviating the immune response and oxidative damage caused by high-level of Clostridium autoethanogenum proteinJiahao Song0Chaozhong Zheng1Qi Hao2Jiayi Huang3Wencheng Huang4Shuyan Chi5Beiping Tan6Wen Huang7Shiwei Xie8Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Province Research Center for Accurate Nutrition and High-Efficiency Feeding of Aquatic Animals, Zhanjiang 524088, China; Key Laboratory of Aquatic Feed Science and Technology for Livestock and Poultry in Southern China, under the Ministry of Agriculture, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Province Research Center for Accurate Nutrition and High-Efficiency Feeding of Aquatic Animals, Zhanjiang 524088, China; Key Laboratory of Aquatic Feed Science and Technology for Livestock and Poultry in Southern China, under the Ministry of Agriculture, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Province Research Center for Accurate Nutrition and High-Efficiency Feeding of Aquatic Animals, Zhanjiang 524088, China; Key Laboratory of Aquatic Feed Science and Technology for Livestock and Poultry in Southern China, under the Ministry of Agriculture, Zhanjiang 524088, ChinaCollaborative Innovation Center of Aquatic Sciences, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Corresponding author.Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Province Research Center for Accurate Nutrition and High-Efficiency Feeding of Aquatic Animals, Zhanjiang 524088, China; Key Laboratory of Aquatic Feed Science and Technology for Livestock and Poultry in Southern China, under the Ministry of Agriculture, Zhanjiang 524088, China; Corresponding author at: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.This study investigates the impact of dietary mineral supplementation in Clostridium autoethanogenum protein (CAP)-containing low-fishmeal diets on growth, lipid metabolic profile, and immune and antioxidant capacity in Litopenaeus vannamei. Three diets were formulated in this experiment. The positive control group (PC) was a high fishmeal protein diet with 25 % fish meal, and CAP was used to replace the low fish meal diet (NC) with 50 % fish meal (12.5 % fishmeal) in the PC group. The third group was added minerals (Mg, Fe, Mn and Se) (HM) in the NC diet. Each group contained 160 shrimp, divided into 4 replicates (initial weight: 1.79 ± 0.02 g). The results showed that after fishmeal was replaced by CAP, the growth performance and antioxidant capacity of shrimp decreased. Challenges to lipid metabolism homeostasis were also observed. Transcriptional analysis revealed upregulation of innate immune genes (dorsal, relish, litaf) in the NC dietary group compared to other groups. After mineral supplementation, antioxidant and immune capacity were enhanced. In addition, correlation analysis showed that there was a significant interaction between midgut morphology and immune genes. In conclusion, mineral supplementation can partially alleviate the oxidative stress and immune imbalance caused by the replacement of fish meal with CAP, but has limited effects on the growth of shrimp.http://www.sciencedirect.com/science/article/pii/S2352513425003771FishmealClostridium autoethanogenum proteinMineralIntestinal healthImmunity |
| spellingShingle | Jiahao Song Chaozhong Zheng Qi Hao Jiayi Huang Wencheng Huang Shuyan Chi Beiping Tan Wen Huang Shiwei Xie Mineral supplementation protected intestinal health in Litopenaeus vannamei by alleviating the immune response and oxidative damage caused by high-level of Clostridium autoethanogenum protein Aquaculture Reports Fishmeal Clostridium autoethanogenum protein Mineral Intestinal health Immunity |
| title | Mineral supplementation protected intestinal health in Litopenaeus vannamei by alleviating the immune response and oxidative damage caused by high-level of Clostridium autoethanogenum protein |
| title_full | Mineral supplementation protected intestinal health in Litopenaeus vannamei by alleviating the immune response and oxidative damage caused by high-level of Clostridium autoethanogenum protein |
| title_fullStr | Mineral supplementation protected intestinal health in Litopenaeus vannamei by alleviating the immune response and oxidative damage caused by high-level of Clostridium autoethanogenum protein |
| title_full_unstemmed | Mineral supplementation protected intestinal health in Litopenaeus vannamei by alleviating the immune response and oxidative damage caused by high-level of Clostridium autoethanogenum protein |
| title_short | Mineral supplementation protected intestinal health in Litopenaeus vannamei by alleviating the immune response and oxidative damage caused by high-level of Clostridium autoethanogenum protein |
| title_sort | mineral supplementation protected intestinal health in litopenaeus vannamei by alleviating the immune response and oxidative damage caused by high level of clostridium autoethanogenum protein |
| topic | Fishmeal Clostridium autoethanogenum protein Mineral Intestinal health Immunity |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003771 |
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