Polyinosinic-polycytidylic acid modulates Porphyromonas gingivalis-induced cell apoptosis via the janus kinase/ signal transducer and activator of transcription signaling pathway
Background/Purpose: Porphyromonas gingivalis (P. gingivalis) has been shown to induce apoptosis in endothelial cells and contribute to the progression of atherosclerosis. While Polyinosinic-polycytidylic acid (Poly (I:C)) is known to activate the innate immune response against infections, its potent...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790224003325 |
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| Summary: | Background/Purpose: Porphyromonas gingivalis (P. gingivalis) has been shown to induce apoptosis in endothelial cells and contribute to the progression of atherosclerosis. While Polyinosinic-polycytidylic acid (Poly (I:C)) is known to activate the innate immune response against infections, its potential interference with P. gingivalis-induced atherosclerosis remains unclear. This study aimed to elucidate the role and underlying mechanisms of Poly (I:C) in mediating human umbilical vein endothelial cells (HUVECs) apoptosis induced by P. gingivalis. Materials and methods: A mice model of atherosclerosis and a model of P. gingivalis-induced bacteremia were established to investigate the effects of Poly (I:C) on P. gingivalis-induced apoptosis in the aortic root, as well as the expression levels of apoptosis-related proteins including Caspase 3, Caspase 9, Bax, and Bcl-2. Subsequently, HUVECs were cultured in vitro to compare cell apoptosis and the expression of these apoptosis-related proteins under stimulation with P. gingivalis, both with and without Poly (I:C) treatment; additionally, the activation status of the JAK/STAT signaling pathway was assessed. Results: The administration of Poly (I:C) diminished apoptosis in the aortic root cells of mice, enhanced the expression of the anti-apoptotic protein Bcl-2, and decreased the levels of Bax, Caspase 3 and 9. Furthermore, Poly (I:C) exhibited similar effects on HUVECs cultured in vitro. Additionally, treatment with Poly (I:C) activated the JAK/STAT signaling pathway, while STAT inhibitor was found to attenuate its effects. Conclusion: Poly (I:C) attenuated P. gingivalis-induced cellular apoptosis, with the involvement of the JAK/STAT signaling pathway in this mechanism. |
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| ISSN: | 1991-7902 |