Tailored Fluorescent Metal–Organic Frameworks Hybrid Membrane Sensor Arrays: Simultaneous and Selective Quantification of Multiple Antibiotics

Abstract Sensor array offers significant potential for rapid, high‐throughput antibiotic detection. However, cross‐reactivity‐based sensor arrays often lack accuracy, despite comprehensive data analysis; while traditional high‐affinity‐based sensors based on antibodies/aptamers frequently suffer fro...

Full description

Saved in:
Bibliographic Details
Main Authors: Tongtong Ma, Qiao Huang, Lei Yuan, Shugang Yan, Yalin Mo, Yibin Ying, Yingchun Fu, Jinming Pan
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Advanced Science
Subjects:
Online Access:https://doi.org/10.1002/advs.202502452
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849720044528861184
author Tongtong Ma
Qiao Huang
Lei Yuan
Shugang Yan
Yalin Mo
Yibin Ying
Yingchun Fu
Jinming Pan
author_facet Tongtong Ma
Qiao Huang
Lei Yuan
Shugang Yan
Yalin Mo
Yibin Ying
Yingchun Fu
Jinming Pan
author_sort Tongtong Ma
collection DOAJ
description Abstract Sensor array offers significant potential for rapid, high‐throughput antibiotic detection. However, cross‐reactivity‐based sensor arrays often lack accuracy, despite comprehensive data analysis; while traditional high‐affinity‐based sensors based on antibodies/aptamers frequently suffer from complicated design and poor robustness. Here, a filterable paper‐based fluorescent metal–organic frameworks (MOFs) sensor array is developed for one‐to‐one recognition and quantification of multiple antibiotics. Three representative MOFs are designed to exceptional affinity and specificity for the target antibiotic. A filtration‐assisted detection enhances sensitivity, achieving parts‐per‐billion (ppb)‐level detection in mixed solutions. The proposed approach integrates recognition and signal generation, streamlined 10‐min process. The robustness of the MOFs also enables direct detection in raw samples containing organic solvents, which is not achievable by conventional methods. Notably, the sensor array can be easily incorporated into a smartphone‐based portable device, coupled with a user‐friendly image analysis applet for one‐step extraction and quantitative detection in chicken samples. Leveraging MOFs’ versatility, this method can be extended to simultaneously detect a broad range of antibiotics, offering the potential for universal, high‐throughput accurate detection of various chemical targets.
format Article
id doaj-art-ce96a8cefaa5469fa42922a16cff9282
institution DOAJ
issn 2198-3844
language English
publishDate 2025-07-01
publisher Wiley
record_format Article
series Advanced Science
spelling doaj-art-ce96a8cefaa5469fa42922a16cff92822025-08-20T03:12:02ZengWileyAdvanced Science2198-38442025-07-011227n/an/a10.1002/advs.202502452Tailored Fluorescent Metal–Organic Frameworks Hybrid Membrane Sensor Arrays: Simultaneous and Selective Quantification of Multiple AntibioticsTongtong Ma0Qiao Huang1Lei Yuan2Shugang Yan3Yalin Mo4Yibin Ying5Yingchun Fu6Jinming Pan7College of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture Zhejiang University Hangzhou 310058 ChinaCollege of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture Zhejiang University Hangzhou 310058 ChinaCollege of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture Zhejiang University Hangzhou 310058 ChinaCollege of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture Zhejiang University Hangzhou 310058 ChinaCollege of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture Zhejiang University Hangzhou 310058 ChinaCollege of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture Zhejiang University Hangzhou 310058 ChinaCollege of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture Zhejiang University Hangzhou 310058 ChinaCollege of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture Zhejiang University Hangzhou 310058 ChinaAbstract Sensor array offers significant potential for rapid, high‐throughput antibiotic detection. However, cross‐reactivity‐based sensor arrays often lack accuracy, despite comprehensive data analysis; while traditional high‐affinity‐based sensors based on antibodies/aptamers frequently suffer from complicated design and poor robustness. Here, a filterable paper‐based fluorescent metal–organic frameworks (MOFs) sensor array is developed for one‐to‐one recognition and quantification of multiple antibiotics. Three representative MOFs are designed to exceptional affinity and specificity for the target antibiotic. A filtration‐assisted detection enhances sensitivity, achieving parts‐per‐billion (ppb)‐level detection in mixed solutions. The proposed approach integrates recognition and signal generation, streamlined 10‐min process. The robustness of the MOFs also enables direct detection in raw samples containing organic solvents, which is not achievable by conventional methods. Notably, the sensor array can be easily incorporated into a smartphone‐based portable device, coupled with a user‐friendly image analysis applet for one‐step extraction and quantitative detection in chicken samples. Leveraging MOFs’ versatility, this method can be extended to simultaneously detect a broad range of antibiotics, offering the potential for universal, high‐throughput accurate detection of various chemical targets.https://doi.org/10.1002/advs.202502452antibioticshybrid membranemetal–organic frameworksensor arraysimultaneous detection
spellingShingle Tongtong Ma
Qiao Huang
Lei Yuan
Shugang Yan
Yalin Mo
Yibin Ying
Yingchun Fu
Jinming Pan
Tailored Fluorescent Metal–Organic Frameworks Hybrid Membrane Sensor Arrays: Simultaneous and Selective Quantification of Multiple Antibiotics
Advanced Science
antibiotics
hybrid membrane
metal–organic framework
sensor array
simultaneous detection
title Tailored Fluorescent Metal–Organic Frameworks Hybrid Membrane Sensor Arrays: Simultaneous and Selective Quantification of Multiple Antibiotics
title_full Tailored Fluorescent Metal–Organic Frameworks Hybrid Membrane Sensor Arrays: Simultaneous and Selective Quantification of Multiple Antibiotics
title_fullStr Tailored Fluorescent Metal–Organic Frameworks Hybrid Membrane Sensor Arrays: Simultaneous and Selective Quantification of Multiple Antibiotics
title_full_unstemmed Tailored Fluorescent Metal–Organic Frameworks Hybrid Membrane Sensor Arrays: Simultaneous and Selective Quantification of Multiple Antibiotics
title_short Tailored Fluorescent Metal–Organic Frameworks Hybrid Membrane Sensor Arrays: Simultaneous and Selective Quantification of Multiple Antibiotics
title_sort tailored fluorescent metal organic frameworks hybrid membrane sensor arrays simultaneous and selective quantification of multiple antibiotics
topic antibiotics
hybrid membrane
metal–organic framework
sensor array
simultaneous detection
url https://doi.org/10.1002/advs.202502452
work_keys_str_mv AT tongtongma tailoredfluorescentmetalorganicframeworkshybridmembranesensorarrayssimultaneousandselectivequantificationofmultipleantibiotics
AT qiaohuang tailoredfluorescentmetalorganicframeworkshybridmembranesensorarrayssimultaneousandselectivequantificationofmultipleantibiotics
AT leiyuan tailoredfluorescentmetalorganicframeworkshybridmembranesensorarrayssimultaneousandselectivequantificationofmultipleantibiotics
AT shugangyan tailoredfluorescentmetalorganicframeworkshybridmembranesensorarrayssimultaneousandselectivequantificationofmultipleantibiotics
AT yalinmo tailoredfluorescentmetalorganicframeworkshybridmembranesensorarrayssimultaneousandselectivequantificationofmultipleantibiotics
AT yibinying tailoredfluorescentmetalorganicframeworkshybridmembranesensorarrayssimultaneousandselectivequantificationofmultipleantibiotics
AT yingchunfu tailoredfluorescentmetalorganicframeworkshybridmembranesensorarrayssimultaneousandselectivequantificationofmultipleantibiotics
AT jinmingpan tailoredfluorescentmetalorganicframeworkshybridmembranesensorarrayssimultaneousandselectivequantificationofmultipleantibiotics