Polymorphism and phylogenetic analysis of ribosomal protein genes of Candidatus Liberibacter asiaticus
Ribosomal protein genes of Candidatus Liberibacter asiaticus from six different geographical regions were amplified, and the restriction fragment length polymorphism (RFLP) of PCR products were analyzed. Then, the sequences of ribosomal protein genes were aligned with software DNAman and NCBI Blast,...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2011-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.02.002 |
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| Summary: | Ribosomal protein genes of Candidatus Liberibacter asiaticus from six different geographical regions were amplified, and the restriction fragment length polymorphism (RFLP) of PCR products were analyzed. Then, the sequences of ribosomal protein genes were aligned with software DNAman and NCBI Blast, and the phylogenetic tree was constructed. The results showed that the ribosomal protein genes of six isolates had different RFLP fingerprints and showed some polymorphism, and the differences existed in the nucleotide sequences and amino acid sequences of ribosomal protein genes of different isolates, while the homology between each other was very high. Their homology level was from 99.0% to 100% with other Ca. L. asiaticus in GenBank, 85% with Ca. L. africanus, and 77% to 80% with Ca. L. americanus, respectively. Phylogenetic analysis suggested that all Ca. L. asiaticus were clustered into one group, and then clustered in a larger group as Candidatus Liberibacter with Ca. L. africanus and Ca. L. americanus. |
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| ISSN: | 1008-9209 2097-5155 |