Production of multi-subunit proteins in CHO cells by transposase-mediated integration of subunit-splitting vectors

Production of multi-subunit proteins in CHO cells by transposase-mediated integration of subunit-splitting vectors

Abstract The Chinese hamster ovary (CHO) cell line is a widely employed system for producing therapeutic proteins. In our previous study, we established an efficient method for generating stable cell lines by utilizing the Tol2 transposon system, combined with cycloheximide (CHX) resistance as a sel...

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Bibliographic Details
Main Authors:	Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara, Koichi Kawakami
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-05-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-025-03301-3
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